The 2023 MDPI Annual Report has
been released!
 
17 pages, 13213 KiB  
Article
Combining RNAscope, Immunohistochemistry (IHC) and Digital Image Analysis to Assess Podoplanin (PDPN) Protein and PDPN_mRNA Expression on Formalin-Fixed Paraffin-Embedded Normal Human Placenta Tissues
by Larisa Cristina Tomescu, Andrei Alexandru Cosma, Mihaela Pasca Fenesan, Eugen Melnic, Vergil Petrovici, Simona Sarb, Monica Chis, Ioan Sas, Domenico Ribatti, Anca Maria Cimpean and Florica Ramona Dorobantu
Curr. Issues Mol. Biol. 2024, 46(6), 5161-5177; https://doi.org/10.3390/cimb46060310 (registering DOI) - 24 May 2024
Abstract
The expression and function of podoplanin (PDPN) in the normal human placenta has been debated in placental evaluation. This study emphasizes the importance of a multimodal approach of PDPN expression in normal human placentas. A complete examination is performed using immunohistochemistry, RNAscope and [...] Read more.
The expression and function of podoplanin (PDPN) in the normal human placenta has been debated in placental evaluation. This study emphasizes the importance of a multimodal approach of PDPN expression in normal human placentas. A complete examination is performed using immunohistochemistry, RNAscope and automated Digital Image examination (DIA) interpretation. QuPath DIA-based analysis automatically generated the stromal and histological scores of PDPN expression for immunohistochemistry and RNAscope stains. The umbilical cord’s isolated fibroblasts and luminal structures expressed PDPN protein and PDPN_mRNA. RNAscope detected PDPN_mRNA upregulation in syncytial placental knots trophoblastic cells, but immunohistochemistry did not certify this at the protein level. The study found a significant correlation between the IHC and RNAscope H-Score (p = 0.033) and Allred Score (p = 0.05). A successful multimodal strategy for PDPN assessment in human placentas confirmed PDPN expression heterogeneity in the full-term human normal placenta and umbilical cord at the protein and mRNA level. In placental syncytial knots trophoblastic cells, PDPN showed mRNA overexpression, suggesting a potential role in placenta maturation. Full article
(This article belongs to the Special Issue Molecular Research in Reproductive Biology, 2nd Edition)
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20 pages, 1297 KiB  
Article
A Diachronic Overview of the Prepositional Accusative in Portuguese
by Ana Regina Calindro
Languages 2024, 9(6), 194; https://doi.org/10.3390/languages9060194 (registering DOI) - 24 May 2024
Abstract
One of the several differences between Modern European Portuguese (EP) and Modern Brazilian Portuguese (BP) is the prepositional expression of complements licensed by the preposition a. While in EP the preposition a occurs in several contexts, this element has been substituted by [...] Read more.
One of the several differences between Modern European Portuguese (EP) and Modern Brazilian Portuguese (BP) is the prepositional expression of complements licensed by the preposition a. While in EP the preposition a occurs in several contexts, this element has been substituted by other strategies in BP, as is extensively discussed in the literature. The aim of this paper is to investigate the historical behavior of a-marked prepositional accusatives (PP-ACC) in Portuguese. In order to do so, a search was conducted for PP-ACCs in the Historical Portuguese Corpus Tycho Brahe. The results showed an increase of PP-ACCs in the 17th century, followed by a decrease in the 18th century. Thereafter, unmarked accusatives (NP-ACC) were analyzed in the corpus, which resulted in 7756 sentences, contrasting with 624 PP-ACCs in the same contexts. This result shows that the a-marked accusative is far less common than bare accusatives in Historical Portuguese. Psych verbs, however, behaved differently, showing a constant increase in PP-ACCs. In EP, the preposition a still introduces Experiencer arguments in structures with some psych verbs (O vinho agradou ao João—lit. ‘The wine pleased ‘to’ John’). In BP, the preposition a has disappeared in psych predicates (O vinho agradou Ø o João—‘The wine pleased John’). In both Modern EP and BP, most PP-ACCs have become typical unmarked direct objects. In the context of psych verbs, however, structural accusative assignment has shifted to structural dative Case in Modern EP, so as to ascertain the interpretation of the Experiencer in the internal argument via the preposition a. While in Modern BP, the argument is not overtly marked since it receives inherent accusative case in the derivation. Full article
(This article belongs to the Special Issue Investigating Language Variation and Change in Portuguese)
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17 pages, 11637 KiB  
Article
Evaluation of Algal Control Measures in Eutrophic Reservoirs Based on Aquatic Ecosystem Models
by Zhen Zheng, Tingting Liao, Yafeng Lin, Xueyi Zhu and Haobin Meng
Water 2024, 16(11), 1494; https://doi.org/10.3390/w16111494 (registering DOI) - 24 May 2024
Abstract
The frequency of freshwater cyanobacterial blooms is increasing globally due to climate change and eutrophication, particularly in reservoirs. Reservoir ecosystems exhibit unique characteristics, and there is a complex relationship between factors such as light, temperature, nutrient salts, hydrology, and algal growth. The impact [...] Read more.
The frequency of freshwater cyanobacterial blooms is increasing globally due to climate change and eutrophication, particularly in reservoirs. Reservoir ecosystems exhibit unique characteristics, and there is a complex relationship between factors such as light, temperature, nutrient salts, hydrology, and algal growth. The impact of the other factors on algal growth varies significantly among different reservoirs. Thus, it is crucial to assess the effectiveness of various algal control measures implemented in different reservoirs. This study conducted a comprehensive assessment by establishing a eutrophication model for the Shanzi Reservoir in Fuzhou City. The model incorporated meteorology, hydrology, carbon dynamics, nutrient cycling, and biological communities. The effectiveness of diverse management measures was systematically evaluated. The findings demonstrate that increasing the water level, reducing nutrient salts in sediments, and implementing ecological fish stocking effectively suppressed algal growth to varying degrees and improved nitrogen and phosphorus levels. Lower water levels and ecological fish stocking had a significant impact on algal reproduction, while sediment reduction had a minimal effect. Conversely, lower water levels and ecological fish stocking did not significantly improve nitrogen and phosphorus concentrations in the reservoir, whereas sediment reduction had a noticeable effect. Consequently, the management strategies for the Shanzi Reservoir should prioritize external control measures and the implementation of ecological fish stocking. Full article
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16 pages, 1986 KiB  
Article
Reducing Driving Risk Factors in Adolescents with Attention Deficit Hyperactivity Disorder (ADHD): Insights from EEG and Eye-Tracking Analysis
by Anat Keren, Orit Fisher, Anwar Hamde, Shlomit Tsafrir and Navah Z. Ratzon
Sensors 2024, 24(11), 3319; https://doi.org/10.3390/s24113319 (registering DOI) - 23 May 2024
Abstract
Adolescents with attention deficit hyperactivity disorder (ADHD) face significant driving challenges due to deficits in attention and executive functioning, elevating their road risks. Previous interventions targeting driving safety among this cohort have typically addressed isolated aspects (e.g., cognitive or behavioral factors) or relied [...] Read more.
Adolescents with attention deficit hyperactivity disorder (ADHD) face significant driving challenges due to deficits in attention and executive functioning, elevating their road risks. Previous interventions targeting driving safety among this cohort have typically addressed isolated aspects (e.g., cognitive or behavioral factors) or relied on uniform solutions. However, these approaches often overlook this population’s diverse needs. This study introduces the “Drive-Fun” innovative intervention (DFI), aimed at enhancing driving skills among this vulnerable population. The intervention was tested in a pilot study including 30 adolescents aged 15–18, comparing three groups: DFI, an educational intervention, and a control group with no treatment. Assessments included a driving simulator, EEG, and Tobii Pro Glasses 2. Evaluation was conducted pre- and post-intervention and at a 3-month follow-up. Results indicated that the DFI group significantly improved in the simulated driving performance, attentional effort, and focused gaze time. The findings underscore that holistic strategies with personalized, comprehensive approaches for adolescents with ADHD are particularly effective in improving driving performance. These outcomes not only affirm the feasibility of the DFI but also highlight the critical role of sensor technologies in accurately measuring and enhancing simulator driving performance in adolescents with ADHD. Outcomes suggest a promising direction for future research and application. Full article
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15 pages, 3020 KiB  
Article
The Possible Role of Mycotoxins in the Pathogenesis of Endometrial Cancer
by Márkó Unicsovics, Zsófia Molnár, Miklós Mézes, Katalin Posta, György Nagyéri, Szabolcs Várbíró, Nándor Ács, Levente Sára and Zsuzsanna Szőke
Toxins 2024, 16(6), 236; https://doi.org/10.3390/toxins16060236 - 23 May 2024
Abstract
Endometrial cancer is one of the most common cancer types among women. Many factors can contribute to the development of this disease, including environmental factors and, thus, eating habits. Our study aims to determine the levels of various mycotoxins and their metabolites in [...] Read more.
Endometrial cancer is one of the most common cancer types among women. Many factors can contribute to the development of this disease, including environmental factors and, thus, eating habits. Our study aims to determine the levels of various mycotoxins and their metabolites in the blood serum and endometrial tissue samples of participants with previously proven endometrial cancer and to find possible contributions to cancer development. In the cohort clinical trial, 52 participants aged between 44 and 86 were studied. The participants were divided into two groups: patients or matched controls. All patients had previously histologically diagnosed endometrial cancer. The cancer patients were divided into low-grade endometrioid and low- plus high-grade endometrioid groups. Controls had no history of endometrial malignancy or premalignancy. Blood serum and endometrial tissue samples were obtained from all study patients. We compared the concentrations of total Aflatoxins (Afs), Deoxynivalenol (DON), Ochratoxin-A (OTA), T2-toxin and HT2 toxin (T2/HT2 toxin), Zearalenone (ZEN), alpha-Zearalenol (α-ZOL), and Fumonisin B1 (FB1) in the serum and endometrium between the different study groups. As a result, we can see a significant correlation between the higher levels of Afs and zearalenone and the presence of endometrial cancer. In the case of Afs, DON, OTA, T2/HT2 toxins, ZEN, and alpha-ZOL, we measured higher endometrial concentrations than in serum. Considering the effect of mycotoxins and eating habits on cancer development, our results might lead to further research exploring the relationship between certain mycotoxins and endometrium cancer. Full article
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11 pages, 243 KiB  
Article
Collective and Substantial: Human Dignity beyond Individualism
by Justin Conway
Religions 2024, 15(6), 639; https://doi.org/10.3390/rel15060639 - 23 May 2024
Abstract
This paper uses Pope Francis’s Fratelli Tutti to develop traditional Catholic depictions of human dignity in both theory and practice. Black sociopolitical thought and social movement organizing are brought into conversation with theologies and philosophies that reflect on what gives life integrity. I [...] Read more.
This paper uses Pope Francis’s Fratelli Tutti to develop traditional Catholic depictions of human dignity in both theory and practice. Black sociopolitical thought and social movement organizing are brought into conversation with theologies and philosophies that reflect on what gives life integrity. I posit that the substantialist (ontological) interpretation of human dignity logically extends from individuals to collectives. Further, I suggest religious leaders in the United States should emphasize this collective form of substantialism alongside the relational (Trinitarian) and functional (creative) interpretations of human dignity, thus identifying collectives as thematizing divine likeness and separating the notion of human dignity from individualism. Full article
(This article belongs to the Special Issue Reimagining Catholic Ethics Today)
20 pages, 3139 KiB  
Article
From Stupa to Pagoda: Re-Examining the Sinification and Transformation of Buddhist Monuments from Indian Origins
by Young-Jae Kim
Religions 2024, 15(6), 640; https://doi.org/10.3390/rel15060640 (registering DOI) - 23 May 2024
Abstract
This study conducts a comparative analysis of Buddhist sacred structures throughout Asia, focusing on the historical development, regional disparities, and the cultural sinification process of stūpas, caityas, and pagodas. Specifically, it delves into the origins, definitions, and terminologies of early Buddhist monuments, such [...] Read more.
This study conducts a comparative analysis of Buddhist sacred structures throughout Asia, focusing on the historical development, regional disparities, and the cultural sinification process of stūpas, caityas, and pagodas. Specifically, it delves into the origins, definitions, and terminologies of early Buddhist monuments, such as stūpas/mahācetiyas and caityas/cetiyas, emphasizing their Indian origins. The research further explores the adaptation and reinterpretation of these original Indian concepts as they spread to East Asia, morphing into new forms, such as pagodas and Buddha halls. It examines the subtle shifts in terminology and the altered meanings and functions of these monuments, from their Indian origins to their sinified representations in East Asia. The transformation of Indian Buddhist monuments through local culture and technology into East Asian architectural forms is investigated, offering a detailed perspective on the dynamic transformation of sacred spaces in Buddhism. This illustrates the religion’s adaptability and integration with the local cultures of ancient East Asia. By analyzing the terminologies and symbolic meanings associated with the architectural transition from stūpa to pagoda, the study argues that sinicized ritual spaces in East Asia have adopted architectural types from pre-Buddhist traditions to represent Indian spaces, thereby highlighting the nuanced changes and the continuous adaptation of sacred Buddhist architecture. Full article
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13 pages, 408 KiB  
Article
Painlevé Analysis of the Traveling Wave Reduction of the Third-Order Derivative Nonlinear Schrödinger Equation
by Nikolay A. Kudryashov and Sofia F. Lavrova
Mathematics 2024, 12(11), 1632; https://doi.org/10.3390/math12111632 - 23 May 2024
Abstract
The second partial differential equation from the Kaup–Newell hierarchy is considered. This equation can be employed to model pulse propagation in optical fiber, wave propagation in plasma, or high waves in the deep ocean. The integrability of the explored equation in traveling wave [...] Read more.
The second partial differential equation from the Kaup–Newell hierarchy is considered. This equation can be employed to model pulse propagation in optical fiber, wave propagation in plasma, or high waves in the deep ocean. The integrability of the explored equation in traveling wave variables is investigated using the Painlevé test. Periodic and solitary wave solutions of the studied equation are presented. The investigated equation belongs to the class of generalized nonlinear Schrödinger equations and may be used for the description of optical solitons in a nonlinear medium. Full article
(This article belongs to the Special Issue Exact Solutions and Numerical Solutions of Differential Equations)
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21 pages, 9145 KiB  
Article
Thermal Comfort of Nelore Cattle (Bos indicus) Managed in Silvopastoral and Traditional Systems Associated with Rumination in a Humid Tropical Environment in the Eastern Amazon, Brazil
by Welligton Conceição da Silva, Jamile Andréa Rodrigues da Silva, Lucieta Guerreiro Martorano, Éder Bruno Rebelo da Silva, Katarina Cardoso de Carvalho, Carlos Eduardo Lima Sousa, Kedson Alessandri Lobo Neves, Raimundo Nonato Colares Camargo Júnior, Tatiane Silva Belo, Ana Gizela Souza de Santos, Cláudio Vieira de Araújo, Leonel António Joaquim, Thomaz Cyro Guimarães de Carvalho Rodrigues and José de Brito Lourenço-Júnior
Vet. Sci. 2024, 11(6), 236; https://doi.org/10.3390/vetsci11060236 (registering DOI) - 23 May 2024
Abstract
The objective of this study was to evaluate the thermal comfort of Nelore cattle (Bos indicus) managed in silvopastoral and traditional systems associated with rumination behavior in a humid tropical environment in the Eastern Amazon, Brazil. The study was carried out [...] Read more.
The objective of this study was to evaluate the thermal comfort of Nelore cattle (Bos indicus) managed in silvopastoral and traditional systems associated with rumination behavior in a humid tropical environment in the Eastern Amazon, Brazil. The study was carried out on a rural property in Mojuí dos Campos, Pará, Brazil, during the transition period of the year, from June to July 2023. Over these two months, six consecutive data collection days were held. We selected 20 clinically healthy non-castrated male Nelore cattle, aged between 18 and 20 months, with an average weight of 250 kg and body condition score of 3.5 (1–5). These animals were randomly divided into two groups: traditional system (TS) and silvopastoral system (SS). The physiological variables evaluated included RR, RT and BST. The variables were analyzed using the linear mixed model. For agrometeorological variables, higher values were observed between 10:00 a.m. (33 °C) and 6:00 p.m. (30 °C), with the highest temperature observed at 4:00 p.m. (40 °C). The RR showed interactions (p = 0.0214) between systems and times; in general, higher RR were obtained in the Traditional. The animals’ RT showed no significant difference (p < 0.05) between the production systems, but there was a statistically significant difference in relation to the time of collection (p < 0.0001). In the BGHI, it was possible to observe that there was mild stress in the period from 22:00 at night to 6:00 in the morning and moderate stress in the period of greatest increase in temperature, from 10:00 in the morning to 18:00 at night. BST showed no statistical difference between the regions studied or between the SP (35.6 °C) and TS (36.25 °C) systems. RT in the TS showed a positive correlation with AT (r = 0.31507; p = 0.0477). RT in the SP showed a positive correlation with THI (r = 35583; p = 0.0242). On the other hand, RT in the SP (r = 0.42873; p = 0.0058) and ST (r = 0.51015; p = 0.0008) showed a positive correlation with BGHI. RR in the TS showed a positive correlation with BGHI (r = 0.44908; p = 0.0037). The greatest amounts of rumination were carried out by animals in the SP system, generally ruminating lying down (p < 0.05). With regard to rumination behavior in the morning and afternoon, there were higher numbers of WS and LD in the TS (p > 0.05). Most of the time, the cattle were LD during the morning and afternoon shifts, and at night and dawn they were WS in the TS. Therefore, the SP offers more thermal comfort advantages compared to the TS system. Full article
25 pages, 2588 KiB  
Review
Veterinary Drug Residues in the Food Chain as an Emerging Public Health Threat: Sources, Analytical Methods, Health Impacts, and Preventive Measures
by Hazim O. Khalifa, Lamek Shikoray, Mohamed-Yousif Ibrahim Mohamed, Ihab Habib and Tetsuya Matsumoto
Foods 2024, 13(11), 1629; https://doi.org/10.3390/foods13111629 - 23 May 2024
Abstract
Veterinary medications are necessary for both contemporary animal husbandry and food production, but their residues can linger in foods obtained from animals and pose a dangerous human risk. In this review, we aim to highlight the sources, occurrence, human exposure pathways, and human [...] Read more.
Veterinary medications are necessary for both contemporary animal husbandry and food production, but their residues can linger in foods obtained from animals and pose a dangerous human risk. In this review, we aim to highlight the sources, occurrence, human exposure pathways, and human health effects of drug residues in food-animal products. Following the usage of veterinary medications, pharmacologically active compounds known as drug residues can be found in food, the environment, or animals. They can cause major health concerns to people, including antibiotic resistance development, the development of cancer, teratogenic effects, hypersensitivity, and disruption of normal intestinal flora. Drug residues in animal products can originate from variety of sources, including water or food contamination, extra-label drug use, and ignoring drug withdrawal periods. This review also examines how humans can be exposed to drug residues through drinking water, food, air, and dust, and discusses various analytical techniques for identifying these residues in food. Furthermore, we suggest some potential solutions to prevent or reduce drug residues in animal products and human exposure pathways, such as implementing withdrawal periods, monitoring programs, education campaigns, and new technologies that are crucial for safeguarding public health. This review underscores the urgency of addressing veterinary drug residues as a significant and emerging public health threat, calling for collaborative efforts from researchers, policymakers, and industry stakeholders to develop sustainable solutions that ensure the safety of the global food supply chain. Full article
(This article belongs to the Special Issue Toxic Residues and Risk Assessment in Food Safety)
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21 pages, 1270 KiB  
Article
The Schwarzian Approach in Sturm–Liouville Problems
by Nektarios Vlahakis
Symmetry 2024, 16(6), 648; https://doi.org/10.3390/sym16060648 - 23 May 2024
Abstract
A novel method for finding the eigenvalues of a Sturm–Liouville problem is developed. Following the minimalist approach, the problem is transformed to a single first-order differential equation with appropriate boundary conditions. Although the resulting equation is nonlinear, its form allows us to find [...] Read more.
A novel method for finding the eigenvalues of a Sturm–Liouville problem is developed. Following the minimalist approach, the problem is transformed to a single first-order differential equation with appropriate boundary conditions. Although the resulting equation is nonlinear, its form allows us to find the general solution by adding a second part to a particular solution. This splitting of the general solution into two parts involves the Schwarzian derivative: hence, the name of the approach. The eigenvalues that correspond to acceptable solutions can be found by requiring the second part to correct the asymptotically diverging behavior of the particular solution. The method can be applied to many different areas of physics, such as the Schrödinger equation in quantum mechanics and stability problems in fluid dynamics. Examples are presented. Full article
(This article belongs to the Special Issue Feature Papers in 'Physics' Section 2024)
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18 pages, 1663 KiB  
Article
The Development of a Cold-Chain-Packaging Risk Management Model Based on Fuzzy Bayesian Network
by Tianran Ren, Jun Ren and Dante Ben Matellini
Appl. Sci. 2024, 14(11), 4446; https://doi.org/10.3390/app14114446 - 23 May 2024
Abstract
Cold chain packaging faces high levels of uncertainty due to its complex nature and dynamic environments during transportation, and the importance of safety and risk management. This study aims to propose a risk assessment model for cold chain packaging based on a fuzzy [...] Read more.
Cold chain packaging faces high levels of uncertainty due to its complex nature and dynamic environments during transportation, and the importance of safety and risk management. This study aims to propose a risk assessment model for cold chain packaging based on a fuzzy Bayesian Network. A case study on vaccine cold chain shipping containers is conducted for the illustration of the risk management model, and risk factors like container risk, packaging risk, monitoring risk and handling risk, occupational and product safety risk have been identified and assessed. This paper will add new knowledge to the field of cold chain packaging, which still lacks research attention, and will be useful for industry managers to gain a better understand of the dynamics and risks of cold chain packaging. Full article
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22 pages, 2382 KiB  
Article
Unraveling the Potential of Attentive Bi-LSTM for Accurate Obesity Prognosis: Advancing Public Health towards Sustainable Cities
by Hina Ayub, Murad-Ali Khan, Syed Shehryar Ali Naqvi, Muhammad Faseeh, Jungsuk Kim, Asif Mehmood and Young-Jin Kim
Bioengineering 2024, 11(6), 533; https://doi.org/10.3390/bioengineering11060533 - 23 May 2024
Abstract
The global prevalence of obesity presents a pressing challenge to public health and healthcare systems, necessitating accurate prediction and understanding for effective prevention and management strategies. This article addresses the need for improved obesity prediction models by conducting a comprehensive analysis of existing [...] Read more.
The global prevalence of obesity presents a pressing challenge to public health and healthcare systems, necessitating accurate prediction and understanding for effective prevention and management strategies. This article addresses the need for improved obesity prediction models by conducting a comprehensive analysis of existing machine learning (ML) and deep learning (DL) approaches. This study introduces a novel hybrid model, Attention-based Bi-LSTM (ABi-LSTM), which integrates attention mechanisms with bidirectional Long Short-Term Memory (Bi-LSTM) networks to enhance interpretability and performance in obesity prediction. Our study fills a crucial gap by bridging healthcare and urban planning domains, offering insights into data-driven approaches to promote healthier living within urban environments. The proposed ABi-LSTM model demonstrates exceptional performance, achieving a remarkable accuracy of 96.5% in predicting obesity levels. Comparative analysis showcases its superiority over conventional approaches, with superior precision, recall, and overall classification balance. This study highlights significant advancements in predictive accuracy and positions the ABi-LSTM model as a pioneering solution for accurate obesity prognosis. The implications extend beyond healthcare, offering a precise tool to address the global obesity epidemic and foster sustainable development in smart cities. Full article
(This article belongs to the Special Issue Intelligent IoMT Systems for Brain–Computer Interface)
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12 pages, 1131 KiB  
Article
The Impact of Bonding Agents and Bone Defects on the Fracture Resistance of Reattached Vertically Root-Fractured Teeth
by Satheesh B. Haralur, Nasser Mohammed Alshahrani, Saeed Hadi Alafra, Muath Ali Hakami, Omar Abdulaziz AbuMesmar, Mohammed A. Al-Qarni, Saeed M. AlQahtani and Nasser M. Alqahtani
Coatings 2024, 14(6), 661; https://doi.org/10.3390/coatings14060661 (registering DOI) - 23 May 2024
Abstract
Many patients experience vertical root fractures, and clinicians often consider conservative treatment options like reattaching the fractured root segments. The study investigated the impact of different bonding agents on the fracture resistance of rebonded vertically fractured teeth with various alveolar bone defects. Human [...] Read more.
Many patients experience vertical root fractures, and clinicians often consider conservative treatment options like reattaching the fractured root segments. The study investigated the impact of different bonding agents on the fracture resistance of rebonded vertically fractured teeth with various alveolar bone defects. Human premolar teeth with a single root were sectioned and reattached using dual-cure resin cement (DCRC), resin-modified glass ionomer (RMGI), and cyanoacrylate. The reattached teeth were then restored with a resin fiber post, composite resin core, and full veneer metal copings. These teeth were embedded in acrylic blocks with angular, V-shaped, and step-shaped bone defects to simulate various alveolar bone conditions. After subjecting the samples to thermal cycling, the fracture resistance was evaluated using a universal testing machine. Teeth samples reattached with RMGI exhibited a higher average fracture resistance. The study also found that DCRC proved to be an effective bonding agent for VRF teeth. However, cyanoacrylate-rebonded teeth exhibited the lowest fracture resistance. The V-shaped defects had a significant impact on the fracture resistance of reattached VRF teeth, with largely unfavorable fractures observed in these cases. Predominantly favorable fractures were observed in the teeth treated with RMGI. The fracture loads in both RMGI and DCRC groups exceeded the expected masticatory load. Full article
(This article belongs to the Special Issue Surface Properties of Dental Materials and Instruments, 2nd Edition)
19 pages, 3934 KiB  
Article
The ArgR-Regulated ADI Pathway Facilitates the Survival of Vibrio fluvialis under Acidic Conditions
by Qian Cheng, Yu Han, Yue Xiao, Zhe Li, Aiping Qin, Saisen Ji, Biao Kan and Weili Liang
Int. J. Mol. Sci. 2024, 25(11), 5679; https://doi.org/10.3390/ijms25115679 - 23 May 2024
Abstract
Vibrio fluvialis is an emerging foodborne pathogenic bacterium that can cause severe cholera-like diarrhea and various extraintestinal infections, posing challenges to public health and food safety worldwide. The arginine deiminase (ADI) pathway plays an important role in bacterial environmental adaptation and pathogenicity. However, [...] Read more.
Vibrio fluvialis is an emerging foodborne pathogenic bacterium that can cause severe cholera-like diarrhea and various extraintestinal infections, posing challenges to public health and food safety worldwide. The arginine deiminase (ADI) pathway plays an important role in bacterial environmental adaptation and pathogenicity. However, the biological functions and regulatory mechanisms of the pathway in V. fluvialis remain unclear. In this study, we demonstrate that L-arginine upregulates the expression of the ADI gene cluster and promotes the growth of V. fluvialis. The ADI gene cluster, which we proved to be comprised of two operons, arcD and arcACB, significantly enhances the survival of V. fluvialis in acidic environments both in vitro (in culture medium and in macrophage) and in vivo (in mice). The mRNA level and reporter gene fusion analyses revealed that ArgR, a transcriptional factor, is necessary for the activation of both arcD and arcACB transcriptions. Bioinformatic analysis predicted the existence of multiple potential ArgR binding sites at the arcD and arcACB promoter regions that were further confirmed by electrophoretic mobility shift assay, DNase I footprinting, or point mutation analyses. Together, our study provides insights into the important role of the ArgR-ADI pathway in the survival of V. fluvialis under acidic conditions and the detailed molecular mechanism. These findings will deepen our understanding of how environmental changes and gene expression interact to facilitate bacterial adaptations and virulence. Full article
(This article belongs to the Special Issue Current Insights into Nucleic Acids)
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15 pages, 1776 KiB  
Article
Can Hediste diversicolor Speed Up the Breakdown of Cigarette Butts in Marine Sediments?
by Mercedes Conradi, J. Emilio Sánchez-Moyano, Francisco J. Rodríguez-Martín and Javier Bayo
Appl. Sci. 2024, 14(11), 4409; https://doi.org/10.3390/app14114409 (registering DOI) - 23 May 2024
Abstract
Cigarette butts (CBs) are non-biodegradable harmful residues of synthetic origin and are widespread in marine environments around the world. Although environmental factors are often primarily responsible for the fragmentation of microplastics in the marine environment, biotic factors have recently been shown to be [...] Read more.
Cigarette butts (CBs) are non-biodegradable harmful residues of synthetic origin and are widespread in marine environments around the world. Although environmental factors are often primarily responsible for the fragmentation of microplastics in the marine environment, biotic factors have recently been shown to be equally important in plastic debris. This study evaluates the role of the Hediste diversicolor polychaete in the fragmentation of CBs in the marine environment. Polychaetes were exposed to three concentrations of CB (0 (as the control), 0.25, and 1 butt L−1) at two different temperatures (15 °C and 23 °C) for 28 days. At each temperature, aquaria without polychaetes were used to study the effect of the burrowing activity of the polychaete on CB fragmentation. Toxicants analysed from exposed sediments increased their concentration in a dose-dependent manner to the CB concentration at a temperature of 15 °C but not at 23 °C. CBs did not directly decrease Hediste survival, but prolonged elevated temperatures increased the polychaetes’ susceptibility. The negative effects of CBs on burial success and burrowing behaviour could not be offset by the reduced start time caused by elevated temperatures. Regardless of temperature, both the weight loss and physical fragmentation of CBs buried in polychaete-contaminated sediments were significantly higher than those without Hediste, with no differences between the two concentrations tested. FTIR-ATR analysis used to evaluate CB degradation in relation to cellulose acetate decomposition showed a greater degradation of this compound in treatments with Hediste than in those without polychaetes (~2.75 times), but these differences were not significant. This study is a promising initial step for future research, as any factor that facilitates the fragmentation of this prevalent and hazardous waste must be carefully studied to extract the maximum benefit to help to reduce CBs in the marine environment. Full article
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11 pages, 962 KiB  
Review
Exploration of Gene Therapy for Alport Syndrome
by Yafei Zhao, Qimin Zheng and Jingyuan Xie
Biomedicines 2024, 12(6), 1159; https://doi.org/10.3390/biomedicines12061159 - 23 May 2024
Abstract
Alport syndrome is a hereditary disease caused by mutations in the genes encoding the alpha 3, alpha 4, and alpha 5 chains of type IV collagen. It is characterized by hematuria, proteinuria, progressive renal dysfunction, hearing loss, and ocular abnormalities. The main network [...] Read more.
Alport syndrome is a hereditary disease caused by mutations in the genes encoding the alpha 3, alpha 4, and alpha 5 chains of type IV collagen. It is characterized by hematuria, proteinuria, progressive renal dysfunction, hearing loss, and ocular abnormalities. The main network of type IV collagen in the glomerular basement membrane is composed of α3α4α5 heterotrimer. Mutations in these genes can lead to the replacement of this network by an immature network composed of the α1α1α2 heterotrimer. Unfortunately, this immature network is unable to provide normal physical support, resulting in hematuria, proteinuria, and progressive renal dysfunction. Current treatment options for Alport syndrome include angiotensin-converting enzyme inhibitors and angiotensin receptor blockers, which aim to alleviate glomerular filtration pressure, reduce renal injury, and delay the progression of renal dysfunction. However, the effectiveness of these treatments is limited, highlighting the need for novel therapeutic strategies and medications to improve patient outcomes. Gene therapy, which involves the use of genetic material to prevent or treat diseases, holds promise for the treatment of Alport syndrome. This approach may involve the insertion or deletion of whole genes or gene fragments to restore or disrupt gene function or the editing of endogenous genes to correct genetic mutations and restore functional protein synthesis. Recombinant adeno-associated virus (rAAV) vectors have shown significant progress in kidney gene therapy, with several gene therapy drugs based on these vectors reaching clinical application. Despite the challenges posed by the structural characteristics of the kidney, the development of kidney gene therapy using rAAV vectors is making continuous progress. This article provides a review of the current achievements in gene therapy for Alport syndrome and discusses future research directions in this field. Full article
(This article belongs to the Special Issue Genetics and Epigenetics of Kidney Diseases)
13 pages, 4284 KiB  
Article
Promoting Thermal Conductivity of Alumina-Based Composite Materials by Systematically Incorporating Modified Graphene Oxide
by Nawon Lee, Jinsol Park, Nayeon Jang, Sehui Lee, Dayeon Kim, Sanggin Yun, Tae Woo Park, Jun-Hyun Kim and Hyun-Ho Park
Crystals 2024, 14(6), 490; https://doi.org/10.3390/cryst14060490 - 23 May 2024
Abstract
Small amounts of thermally conductive graphene oxide (GO) and modified GO are systematically introduced as a second filler to thermal interface materials (TIMs) consisting of alumina (Al2O3) particles and polydimethylsiloxane (PDMS). The surface of GO is covalently linked with [...] Read more.
Small amounts of thermally conductive graphene oxide (GO) and modified GO are systematically introduced as a second filler to thermal interface materials (TIMs) consisting of alumina (Al2O3) particles and polydimethylsiloxane (PDMS). The surface of GO is covalently linked with an organic moiety, octadecylamine (ODA), to significantly improve the miscibility and dispersity of GO across the TIM matrix. Subsequently, two series of PDMS-Al2O3 composite TIMs are manufactured as a function of GO and ODA-GO content (0.25 wt%–2.5 wt%) to understand the effect of these second additives. The incorporation of GO into the Al2O3-PDMS composite materials generally increases the thermal conductivity (TC), ranging from 18% to 29%. Conversely, the use of ODA-GO further enhances the overall performance of TIMs (22–54%) by facilitating the dispersion degree of GO across the composite matrix. The great improvement in TC is presumably related to the formation of conductive pathways by uniformly integrating 2D-type GO flakes across spherical Al2O3 particle networks. The ability to simply regulate the polarity of the thermally conductive second filler can provide an idea for designing cost-effective and practical TIM-2-type pads that can be commercially applicable in between an integrated heat spreader and a heat sink. Full article
(This article belongs to the Section Hybrid and Composite Crystalline Materials)
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17 pages, 487 KiB  
Systematic Review
Effectiveness of Exercise Programs for Alleviation of Upper Body Pain in Patients with Spinal Cord Injury: A Systematic Review
by Jiyoung Park, Jihyun Kim, Seon-Deok Eun and Dongheon Kang
J. Clin. Med. 2024, 13(11), 3066; https://doi.org/10.3390/jcm13113066 - 23 May 2024
Abstract
(1) Background: Upper body pain, particularly in the limbs and shoulders, is a common symptom among patients with spinal cord injury (SCI) and wheelchair users. Despite the focus on resistance muscle training as a suitable intervention for SCI individuals, findings across different [...] Read more.
(1) Background: Upper body pain, particularly in the limbs and shoulders, is a common symptom among patients with spinal cord injury (SCI) and wheelchair users. Despite the focus on resistance muscle training as a suitable intervention for SCI individuals, findings across different populations and conditions have been inconsistent. (2) Methods: We conducted a systematic review to elucidate the correlations among exercise interventions, muscle strength enhancement, and pain reduction. A comprehensive literature search was performed using the keywords “spinal cord injury,” “pain,” “exercise,” “disability,” “paraplegia,” and “tetraplegia” across the DBpia, EMBASE, PubMed, and Science Direct databases. (3) Results: From 191 identified articles, 13 studies (1 from Korea and 12 from other countries) were selected for analysis. The results indicate that exercise interventions are effective in reducing pain in patients with SCI, with a particular emphasis on alleviating shoulder pain. (4) Conclusion: Exercise is essential for pain reduction in patients with SCI, especially those experiencing shoulder pain. However, there is a notable lack of experimental research focusing primarily on pain. The development of appropriate measurement instruments is crucial for the prevention and relief of pain in this patient population. Full article
(This article belongs to the Special Issue Recent Progress in Rehabilitation Medicine—2nd Edition)
14 pages, 1892 KiB  
Article
Associations between PM2.5 Components and Mortality of Ischemic Stroke, Chronic Obstructive Pulmonary Disease and Diabetes in Beijing, China
by Hao Feng, Yisen Yang, Hong Ye, Jing Xu, Meiduo Zhao, Ye Jin and Shuyang Zhang
Toxics 2024, 12(6), 381; https://doi.org/10.3390/toxics12060381 - 23 May 2024
Abstract
Ischemic stroke (IS), chronic obstructive pulmonary disease (COPD) and diabetes mellitus (DM) account for a large burden of premature deaths. However, few studies have investigated the associations between fine particular matter (PM2.5) components and mortality of IS, COPD and DM. We [...] Read more.
Ischemic stroke (IS), chronic obstructive pulmonary disease (COPD) and diabetes mellitus (DM) account for a large burden of premature deaths. However, few studies have investigated the associations between fine particular matter (PM2.5) components and mortality of IS, COPD and DM. We aimed to examine these associations in Beijing, China. Data on daily mortality, air pollutants and meteorological factors from 2008 to 2011 in Beijing were collected. Daily concentrations of five PM2.5 components, namely, sulfate ion (SO42−), ammonium ion (NH4+), nitrate ion (NO3), organic matter (OM) and black carbon (BC), were obtained from the Tracking Air Pollution (TAP) database in China. The association between PM2.5 components and daily deaths was explored using a quasi-Poisson regression with the distributed lag nonlinear model (DLNM). The average daily concentrations of SO42−, NH4+, NO3, OM and BC were 11.24, 8.37, 12.00, 17.34 and 3.32 μg/m3, respectively. After adjusting for temperature, relative humidity, pressure, particulate matter less than 10 μm in aerodynamic diameter (PM10), nitrogen dioxide (NO2) and sulfur dioxide (SO2), an IQR increase in OM at lag day 2 and lag day 6 was associated with an increased DM mortality risk (RR 1.038; 95% CI: 1.005–1.071) and COPD mortality risk (RR 1.013; 95% CI: 1.001–1.026). An IQR increase in BC at lag day 0 and lag day 6 was associated with increased COPD mortality risk (RR 1.228; 95% CI: 1.017–1.48, RR 1.059; 95% CI: 1.001–1.121). Cumulative exposure to SO42− and NH4+ was associated with an increased mortality risk for IS, with the highest effect found for lag of 0–7 days (RR 1.085; 95% CI: 1.010–1.167, RR 1.083; 95% CI: 1.003–1.169). These effects varied by sex and age group. This study demonstrated associations of short-term exposure to PM2.5 components with increased risk of IS, COPD and DM mortality in the general population. Our study also highlighted susceptible subgroups. Full article
(This article belongs to the Section Air Pollution and Health)
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15 pages, 6038 KiB  
Article
Oyster Peptides Ameliorate Dextran Sulfate Sodium-Induced Ulcerative Colitis via Modulating the Gut Microbiota and Inhibiting the TLR4/NF-κB Pathway
by Haixiang Guo, Wenyin Xie, Zhonghao Ji, Bingbing Wang, Wenzhi Ren, Wei Gao and Bao Yuan
Nutrients 2024, 16(11), 1591; https://doi.org/10.3390/nu16111591 - 23 May 2024
Abstract
Ulcerative colitis (UC) is an inflammatory bowel disease with an increasing prevalence year over year, and the medications used to treat patients with UC clinically have severe side effects. Oyster peptides (OPs) have anti-inflammatory and antioxidant properties as functional foods that can alleviate [...] Read more.
Ulcerative colitis (UC) is an inflammatory bowel disease with an increasing prevalence year over year, and the medications used to treat patients with UC clinically have severe side effects. Oyster peptides (OPs) have anti-inflammatory and antioxidant properties as functional foods that can alleviate a wide range of inflammatory conditions. However, the application of oyster peptides in ulcerative colitis is not well studied. In this work, an animal model of acute colitis was established using 3% dextran sulfate sodium (DSS), and the impact of OP therapy on colitis in mice was examined. Supplementing with OPs prevented DSS-induced colitis from worsening, reduced the expression of oxidative stress and inflammatory markers, and restored the intestinal barrier damage caused by DSS-induced colitis in mice. The 16S rDNA results showed that the OP treatment improved the gut microbiota structure of the UC mice, including increasing microbial diversity, increasing beneficial bacteria, and decreasing harmful bacteria. In the UC mice, the OP therapy decreased the relative abundance of Family_XIII_AD3011_group and Prevotella_9 and increased the relative abundance of Alistipes. In conclusion, OP treatment can inhibit the TLR4/NF-κB pathway and improve the intestinal microbiota in UC mice, which in turn alleviates DSS-induced colitis, providing a reference for the treatment of clinical UC patients. Full article
18 pages, 9164 KiB  
Article
Enhancing Photovoltaic Performance with BaTiO3/MWCNTs Composite Photoelectrodes in Dye-Sensitized Solar Cells
by Carlos Armando Polo Bravo, Brayan Yeraldyn Caceres Osnayo, Jesús Alfredo Chacaltana García, Jesús Plácido Medina Salas, Francisco Gamarra Gómez, Hugo Alfredo Torres Muro, Alberto Bacilio Quispe Cohaila, Ramalinga Viswanathan Mangalaraja and Elisban Juani Sacari Sacari
Crystals 2024, 14(6), 489; https://doi.org/10.3390/cryst14060489 - 23 May 2024
Abstract
Dye-sensitized solar cells (DSSCs) have attracted renewed research interest as a potential low-cost substitute for conventional silicon photovoltaics. This work aims to improve the photovoltaic performance of the DSSCs by incorporating multi-walled carbon nanotubes (MWCNTs) into the BaTiO3 photoelectrode. The pure BaTiO [...] Read more.
Dye-sensitized solar cells (DSSCs) have attracted renewed research interest as a potential low-cost substitute for conventional silicon photovoltaics. This work aims to improve the photovoltaic performance of the DSSCs by incorporating multi-walled carbon nanotubes (MWCNTs) into the BaTiO3 photoelectrode. The pure BaTiO3 and BaTiO3/MWCNT nanocomposites were sensitized with N719 dye and fabricated into solar cell devices for testing. The structural characterization confirmed the successful formation of the nanocomposite with an optimal dispersion at 6% of MWCNT incorporation, beyond which agglomeration effects manifested. The optical analysis verified the modulation of defect states and bandgap engineering induced by the MWCNT network. The morphological studies revealed irregular nanoparticle clusters with embedded nanotubes. Solar cell testing under AM1.5G-simulated sunlight demonstrated a peak power conversion efficiency of 4.044% for 6% of MWCNT doping, constituting a 6-fold increment versus pure BaTiO3 (0.693%). It originated from the simultaneous enhancements in the open-circuit voltage and short-circuit current enabled by the favorable band structure alterations and percolation-assisted charge transport. However, further increasing MWCNT content deteriorated the device metrics, owing to emerging limitations like trapping. The rational integration of multi-walled carbon nanotubes with lead-free ferroelectric metal oxides can contribute to the development of emerging organic-inorganic hybrid solar platforms. Full article
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21 pages, 2728 KiB  
Article
Wearable-Based Integrated System for In-Home Monitoring and Analysis of Nocturnal Enuresis
by Sangyeop Lee, Junhyung Moon, Yong Seung Lee, Seung-chul Shin and Kyoungwoo Lee
Sensors 2024, 24(11), 3330; https://doi.org/10.3390/s24113330 - 23 May 2024
Abstract
Nocturnal enuresis (NE) is involuntary bedwetting during sleep, typically appearing in young children. Despite the potential benefits of the long-term home monitoring of NE patients for research and treatment enhancement, this area remains underexplored. To address this, we propose NEcare, an in-home monitoring [...] Read more.
Nocturnal enuresis (NE) is involuntary bedwetting during sleep, typically appearing in young children. Despite the potential benefits of the long-term home monitoring of NE patients for research and treatment enhancement, this area remains underexplored. To address this, we propose NEcare, an in-home monitoring system that utilizes wearable devices and machine learning techniques. NEcare collects sensor data from an electrocardiogram, body impedance (BI), a three-axis accelerometer, and a three-axis gyroscope to examine bladder volume (BV), heart rate (HR), and periodic limb movements in sleep (PLMS). Additionally, it analyzes the collected NE patient data and supports NE moment estimation using heuristic rules and deep learning techniques. To demonstrate the feasibility of in-home monitoring for NE patients using our wearable system, we used our datasets from 30 in-hospital patients and 4 in-home patients. The results show that NEcare captures expected trends associated with NE occurrences, including BV increase, HR increase, and PLMS appearance. In addition, we studied the machine learning-based NE moment estimation, which could help relieve the burdens of NE patients and their families. Finally, we address the limitations and outline future research directions for the development of wearable systems for NE patients Full article
(This article belongs to the Section Wearables)
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