The 2023 MDPI Annual Report has
been released!
 
24 pages, 2346 KiB  
Article
Simulation on the Separation of Breast Cancer Cells within a Dual-Patterned End Microfluidic Device
by Diganta Dutta, Xavier Palmer, Jung Yul Lim and Surabhi Chandra
Fluids 2024, 9(6), 123; https://doi.org/10.3390/fluids9060123 (registering DOI) - 25 May 2024
Abstract
Microfluidic devices have long been useful for both the modeling and diagnostics of numerous diseases. In the past 20 years, they have been increasingly adopted for helping to study those in the family of breast cancer through characterizing breast cancer cells and advancing [...] Read more.
Microfluidic devices have long been useful for both the modeling and diagnostics of numerous diseases. In the past 20 years, they have been increasingly adopted for helping to study those in the family of breast cancer through characterizing breast cancer cells and advancing treatment research in portable and replicable formats. This paper adds to the body of work concerning cancer-focused microfluidics by proposing a simulation of a hypothetical bi-ended three-pronged device with a single channel and 16 electrodes with 8 pairs under different voltage and frequency regimes using COMSOL. Further, a study was conducted to examine the frequencies most effective for ACEO to separate cancer cells and accompanying particles. The study revealed that the frequency of EF has a more significant impact on the separation of particles than the inlet velocity. Inlet velocity variations while holding the frequency of EF constant resulted in a consistent trend showing a direct proportionality between inlet velocity and net velocity. These findings suggest that optimizing the frequency of EF could lead to more effective particle separation and targeted therapeutic interventions for breast cancer. This study hopefully will help to create targeted therapeutic interventions by bridging the disparity between in vitro and in vivo models. Full article
(This article belongs to the Special Issue Physics and Applications of Microfluidics)
14 pages, 635 KiB  
Article
Evaluation of Gamma-Aminobutyric Acid (GABA) as a Functional Feed Ingredient on Growth Performance, Immune Enhancement, and Disease Resistance in Olive Flounder (Paralichthys olivaceus) under High Stocking Density
by Jinho Bae, Mohammad Moniruzzaman, Hyeong-Woo Je, Seunghan Lee, Wonsuk Choi, Taesun Min, Kang-Woong Kim and Sungchul C. Bai
Antioxidants 2024, 13(6), 647; https://doi.org/10.3390/antiox13060647 (registering DOI) - 25 May 2024
Abstract
Gamma-aminobutyric acid (GABA) is a non-protein amino acid that is found in the brain and central nervous system of animals as an inhibitory neurotransmitter. It has been shown to have a variety of physiological functions, including stress reduction and immune enhancement. This study [...] Read more.
Gamma-aminobutyric acid (GABA) is a non-protein amino acid that is found in the brain and central nervous system of animals as an inhibitory neurotransmitter. It has been shown to have a variety of physiological functions, including stress reduction and immune enhancement. This study investigated the effects of dietary supplementation with GABA on growth, serum biochemistry, innate immunity, and disease resistance in juvenile olive flounders (Paralichthys olivaceus) challenged with Edwardsiella tarda under high-stocking density. A control diet and three experimental diets were prepared, with 150 mg/kg (GABA150), 200 mg/kg (GABA200), and 250 mg/kg (GABA250) of GABA added to each diet, respectively. Each experimental diet was fed to olive flounders in triplicate with an initial weight of 12.75 g ± 0.3 g in 40 L tanks at two stocking densities: normal density (20 fish/tank) and high density (40 fish/tank). After 8 weeks of the feeding trial, growth, feed utilization, whole-body proximate compositions, blood analyses, and non-specific immune responses were measured, and challenge tests were performed. There were no significant differences in the weight gain (WG) and specific growth rate (SGR) among fish fed the GABA-supplemented diets at the two stocking densities. However, the normal-density groups showed significantly higher WG and SGR than the high-density groups (p < 0.05). There was no significant difference in feed efficiency and protein efficiency ratio among all groups. Moreover, there was no significant difference in the whole-body proximate composition analysis (p > 0.05). There were no significant differences in cortisol levels in fish fed the GABA at both densities, but the high-density group showed a significantly higher cortisol than the low-density group. Blood GABA significantly increased in a dose-dependent manner regardless of the density groups (p < 0.05). Superoxide dismutase activity showed significantly higher levels than the control group, but there was no significant effect of the stocking densities in fish fed the GABA diets (p < 0.05). Myeloperoxidase activities in fish fed the GABA200 and GABA250 diets showed significantly higher levels at both of the stocking densities (p < 0.05). Lysozyme activity was significantly higher in the GABA150 group than in the CON, GABA200, and GABA250 groups (p < 0.05). After 15 days of challenge tests with Edwardsiella tarda, the cumulative survival rates of the GABA150, GABA200, and GABA250 groups were significantly higher than that of the CON group (p < 0.05). The results suggested that the optimal dietary GABA level for juvenile olive flounder culture is 150 mg/kg, regardless of rearing density, to enhance growth, immunity, and disease resistance. Full article
(This article belongs to the Special Issue Antioxidants Benefits in Aquaculture 2.0)
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18 pages, 4612 KiB  
Article
Mercury and Selenium Accumulation in the Tissues of Stranded Bottlenose Dolphins (Tursiops truncatus) in Northeast Florida, 2013–2021
by Gretchen K. Bielmyer-Fraser, Julia M. Courville, Ashlen Ward and Mckenna M. Hardie
Animals 2024, 14(11), 1571; https://doi.org/10.3390/ani14111571 (registering DOI) - 25 May 2024
Abstract
Bottlenose dolphins (Tursiops truncatus) are long-lived marine mammals, upper-level predators, and they inhabit near-shore environments, which increases their exposure to pollution. Mercury is a ubiquitous and persistent metal pollutant that can bioaccumulate and biomagnify up the food chain. Dolphins are known [...] Read more.
Bottlenose dolphins (Tursiops truncatus) are long-lived marine mammals, upper-level predators, and they inhabit near-shore environments, which increases their exposure to pollution. Mercury is a ubiquitous and persistent metal pollutant that can bioaccumulate and biomagnify up the food chain. Dolphins are known to accumulate mercury, and limited research has shown that mercury exposure can weaken the immune system of dolphins. The objectives of this study were to assess the mercury concentrations in the tissues (muscle, small intestine, liver) of stranded bottlenose dolphins and to compare the tissue mercury levels in dolphins that were stranded during the 2013–2015 morbillivirus Unusual Mortality Event (UME; immunosuppressed individuals) with the levels of those that were stranded at a normal rate (2016–2021). Selenium has been shown to reduce mercury toxicity in many animals; therefore, tissue selenium concentration and the molar ratio of selenium to mercury were also assessed. The tissue mercury (muscle, liver) and selenium (liver) concentrations increased with the age of the dolphins, with the liver accumulating the highest concentrations. No sex differences were observed in the mercury and selenium concentrations. While differences in tissue mercury concentrations were not observed due to the UME, the selenium accumulation profiles were significantly different between the two time periods. These results suggest that selenium may not have been as protective against mercury toxicity in the bottlenose dolphins that were stranded during the UME, possibly due to infection with morbillivirus. Full article
(This article belongs to the Special Issue Threats to Cetacean Health)
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21 pages, 1990 KiB  
Article
BIM Use to Support the Technical Tasks in Construction Projects: A Case Study
by Wael Abdelhameed and Esam M. H. Ismaeil
Buildings 2024, 14(6), 1534; https://doi.org/10.3390/buildings14061534 (registering DOI) - 25 May 2024
Abstract
BIM use has proved to be inevitable in construction project phases, starting from the project initiation phase and ending with the project closeout phase. Through the use of 4D/5D/6D models in processes of the construction project such as scheduling, bills of quantities (BoQs), [...] Read more.
BIM use has proved to be inevitable in construction project phases, starting from the project initiation phase and ending with the project closeout phase. Through the use of 4D/5D/6D models in processes of the construction project such as scheduling, bills of quantities (BoQs), and environmental analysis, BIM provides massive assistance to all project participants. In addition, BIM has been utilized in facility management through the use of 7D models. These well-established benefits positively impact construction project variables: cost, time, and quality. Despite these invaluable contributions in construction projects, BIM is not extensively applied in the Gulf Cooperation Council (GCC) of Arab countries, especially in small/medium-scale construction projects. BIM use at these project scales is mainly exclusive to the design phases. The present use of BIM in GCC construction projects leads to the missing of numerous opportunities, which negatively affects project delivery methods, project timelines, and, consequently, project budgets. To shed more light on this subject, this research paper attempts to answer emerging questions, such as the following: How do BIM and other software programs support the technical team of construction projects? Do these software programs have a direct impact on construction project management, in terms of project timelines and work changes? Does BIM add unnecessary cost to the project budget? This research paper proposes four technical task groups used in construction project phases to evaluate the use of BIM and the associated software programs, namely technical work, Time and Accuracy, budget, and BoQ. These four task groups, moreover, have been ratified and are the subject of consensus by project managers and senior engineers in GCC construction projects, who provided the research primary data through interviews and field surveys. Qualitative and quantitative analyses were conducted to investigate and analyze the present status of BIM use in construction projects, and subsequently, to establish a basis to propose improvements to support the technical team of construction projects. Two approaches are proposed to overcome the obstacles that hinder BIM adoption in GCC countries. The first one is a top-down approach, in which BIM use and BIM documents are mandatory in construction projects. The second approach is a bottom-up approach, where having more BIM professionals in the GCC market will facilitate the transformation to BIM. Full article
(This article belongs to the Section Construction Management, and Computers & Digitization)
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13 pages, 2245 KiB  
Article
The Structure of Digestive Tract Coordinating Digestion and Respiration in an Air-Breathing Weatherloach, Misgurnus anguillicaudatus
by Zixin Qi, Hongbo Ma, Li Ma and Xuefen Yang
Biology 2024, 13(6), 381; https://doi.org/10.3390/biology13060381 (registering DOI) - 25 May 2024
Abstract
To clarify how the digestive tract of the weatherloach, Misgurnus anguillicaudatus, serves a dual function of digestion and respiration simultaneously, the histological structures of its digestive tract, the passage of digesta and air passing through its intestine and the rate of intestinal [...] Read more.
To clarify how the digestive tract of the weatherloach, Misgurnus anguillicaudatus, serves a dual function of digestion and respiration simultaneously, the histological structures of its digestive tract, the passage of digesta and air passing through its intestine and the rate of intestinal evacuation have been studied. The results indicate that the digestive tract is divided into five functional regions, i.e., esophagus, anterior intestine, middle intestine, posterior intestine and rectum. The diverse intestinal structures have the specialized function of coordinating digestion and respiration. An X-ray barium meal examination showed in the normal breathing state, the contents of the intestine are diffusely semifluid, and air is distributed as bubbles in the dorsal intestine 2 h after feeding. After 5 h, the contents accumulated in the mid and posterior intestine, and gas flowed above the contents as bundles. After 8 h, the intestinal food was basically evacuated. In the intestinal air-breathing restricted group, the contents of the intestine remained diffuse, and a large number of digesta entered and remained in the rectum after 5 h. After the inhibition was relieved, the contents of the rectum were rapidly discharged. Measurement of the intestinal evacuation rate in the intestine showed that the evacuation of the intestinal contents lagged behind that of the normal group in the air-breathing restricted group. Compared to the normal state and inhibited GAB (gastrointestinal air breathing), we could deduce that GAB could promote the movement of the intestine. Full article
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10 pages, 1365 KiB  
Communication
ACOM/TEM and EBSD Microstructure Characterization of ECAE-Processed Zirconium
by Mychelle Vianna Pereira Companhoni, Além-mar Bernardes Gonçalves, Maurizio Ferrante, Vitor Sordi and André Luiz Pinto
Metals 2024, 14(6), 624; https://doi.org/10.3390/met14060624 (registering DOI) - 25 May 2024
Abstract
Materials produced through equal channel angular extrusion (ECAE) may offer enhanced mechanical properties over classic thermomechanical processing like extrusion or rolling. Conventional techniques such as electron backscatter diffraction (EBSD) may be insufficient to properly characterize the microstructure of these materials. Darkfield (DF) transmission [...] Read more.
Materials produced through equal channel angular extrusion (ECAE) may offer enhanced mechanical properties over classic thermomechanical processing like extrusion or rolling. Conventional techniques such as electron backscatter diffraction (EBSD) may be insufficient to properly characterize the microstructure of these materials. Darkfield (DF) transmission electron microscopy (TEM), on the other hand, may lead to erroneous conclusions with respect to grain size. In this work, zirconium was submitted to ECAE deformation through four passes in the Bc route at 350 °C. The microstructure was evaluated through FEG-SEM/EBSD (field emission gun–scanning electron microscopy), DF TEM, and ACOM/TEM (automated crystal orientation mapping in TEM). EBSD revealed that the microstructure was heterogeneous with a few large grains surrounded by a fine submicrometric structure, which was only partially resolved through this technique. The fine structure was, however, described through ACOM/TEM. DF TEM was revealed to be somewhat unreliable when the same region was evaluated through ACOM/TEM. Therefore, a combination of techniques seems to be required for proper characterization. Full article
14 pages, 2301 KiB  
Systematic Review
Right-Sided Aortic Arch: A Computed Tomography Angiography Investigation, A Systematic Review with Meta-Analysis
by George Triantafyllou, Savvas Melissanidis, Marianna Vlychou, George Tsakotos, Nikos Pantazis, Katerina Vassiou, Christos Tsiouris and Maria Piagkou
J. Clin. Med. 2024, 13(11), 3105; https://doi.org/10.3390/jcm13113105 (registering DOI) - 25 May 2024
Abstract
Background/Objectives: The right-sided aortic arch (RAA) is an uncommon variation of the aortic arch (AA), characterized by the aorta crossing over the right main bronchus. In the RAA, the descending aorta can be found on either the right or left side of [...] Read more.
Background/Objectives: The right-sided aortic arch (RAA) is an uncommon variation of the aortic arch (AA), characterized by the aorta crossing over the right main bronchus. In the RAA, the descending aorta can be found on either the right or left side of the spine. The current study comprises a comprehensive retrospective computed tomography angiography (CTA) investigation into the prevalence of the RAA within the Greek population. Additionally, we will conduct a systematic review and meta-analysis to elucidate both common and rare morphological variants of the RAA. This research is significant as it sheds light on the prevalence and characteristics of the RAA in a specific population, providing valuable insights for clinical practice. Methods: Two hundred CTAs were meticulously investigated for the presence of a RAA. In addition, the PubMed, Google Scholar, and Scopus online databases were thoroughly searched for studies referring to the AA morphology. The R programming language and RStudio were used for the pooled prevalence meta-analysis, while several subgroup analyses were conducted. Results: Original study: A unique case of 200 CTAs (0.5%) was identified with an uncommon morphology. The following branches emanated from the RAA under the sequence: the right subclavian artery (RSA), the right common carotid artery (RCCA), the left common carotid artery (LCCA), and the left vertebral artery (LVA) in common origin with the aberrant left subclavian artery (ALSA). The ALSA originated from a diverticulum (of Kommerell) and followed a retroesophageal course. Systematic Review and Meta-Analysis: Sixty-two studies (72,187 total cases) met the inclusion criteria. The pooled prevalence of the RAA with a mirror-image morphology was estimated at 0.07%, and the RAA with an ALSA was estimated at <0.01%. Conclusions: AA anomalies, specifically the RAA, raise clinical interest due to their coexistence with developmental heart anomalies and possible interventional complications. Congenital heart anomalies, such as the Tetralogy of Fallot and patent foramen ovale, coexisted with RAA mirror-image morphology. Full article
24 pages, 684 KiB  
Article
Integrating Modular Design Concepts for Enhanced Efficiency in Digital and Sustainable Manufacturing: A Literature Review
by Marc-Antoine Roy and Georges Abdul-Nour
Appl. Sci. 2024, 14(11), 4539; https://doi.org/10.3390/app14114539 (registering DOI) - 25 May 2024
Abstract
Small- and medium-sized manufacturing enterprises (SMMEs) face intense competitiveness, necessitating ever greater productivity. Enterprises struggle to meet the demand for customized products while maintaining their productivity. The transition from mass customization (MC) to mass personalization (MPe) leads to a further increase in product [...] Read more.
Small- and medium-sized manufacturing enterprises (SMMEs) face intense competitiveness, necessitating ever greater productivity. Enterprises struggle to meet the demand for customized products while maintaining their productivity. The transition from mass customization (MC) to mass personalization (MPe) leads to a further increase in product variety and, thus, complexity. Digital transformation alone is not sufficient to achieve MPe and traditional adoption of modularity no longer ensures enterprise competitiveness in this context of increased variety. The synergy between modularity concepts could enhance the efficiency of this design strategy. This study is part of a research plan to develop an effective modularity implementation strategy addressing MPe. The aim of this article is to identify the main concepts and tools to be considered in an implementation strategy. Concepts and tools are grouped into four combinations according to the level of product variety in different production strategies. This preliminary work serves as the foundational research for a larger research plan aimed at adapting and validating a modular product development strategy that incorporates these modularity concepts. Full article
(This article belongs to the Special Issue Digital and Sustainable Manufacturing in Industry 4.0)
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21 pages, 18819 KiB  
Review
Congenital Lung Malformations: A Pictorial Review of Imaging Findings and a Practical Guide for Diagnosis
by Giovanna Cancemi, Giulio Distefano, Gioele Vitaliti, Dario Milazzo, Giuseppe Terzo, Giuseppe Belfiore, Vincenzo Di Benedetto, Maria Grazia Scuderi, Maria Coronella, Andrea Giovanni Musumeci, Daniele Grippaldi, Letizia Antonella Mauro, Pietro Valerio Foti, Antonio Basile and Stefano Palmucci
Children 2024, 11(6), 638; https://doi.org/10.3390/children11060638 (registering DOI) - 25 May 2024
Abstract
The term congenital lung malformation (CLM) is used to describe a wide range of pathological conditions with different imaging and clinical manifestations. These anomalies stem from abnormal embryological lung development, potentially occurring across various stages of prenatal life. Their natural history can be [...] Read more.
The term congenital lung malformation (CLM) is used to describe a wide range of pathological conditions with different imaging and clinical manifestations. These anomalies stem from abnormal embryological lung development, potentially occurring across various stages of prenatal life. Their natural history can be variable, presenting in a wide range of severity levels and encompassing asymptomatic individuals who remain so until adulthood, as well as those who experience respiratory distress in the neonatal period. Through the PubMed database, we performed an extensive review of the literature in the fields of congenital lung abnormalities, including their diagnostic approach and findings. From our RIS-PACS database, we have selected cases with a final diagnosis of congenital lung malformation. Different diagnostic approaches have been selected, including clinical cases studied using plain radiograph, CT scan, prenatal ultrasound, and MR images. The most encountered anomalies can be classified into three categories: bronchopulmonary anomalies (congenital pulmonary airway malformations (CPAMs), congenital lobar hyperinflation, bronchial atresia, and bronchogenic cysts), vascular anomalies (arteriovenous malformation), and combined lung and vascular anomalies (scimitar syndrome and bronchopulmonary sequestration). CLM causes significant morbidity and mortality; therefore, the recognition of these abnormalities is necessary for optimal prenatal counseling and early peri- and postnatal management. This pictorial review aims to report relevant imaging findings in order to offer some clues for differential diagnosis both for radiologists and pediatric consultants. Full article
(This article belongs to the Special Issue Research Progress of Lung and Thoracic Abnormalities in Children)
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10 pages, 2231 KiB  
Article
Chest Dynamic MRI as Early Biomarker of Respiratory Impairment in Amyotrophic Lateral Sclerosis Patients: A Pilot Study
by Francesco Barbato, Alessandro Bombaci, Giovanni Colacicco, Giorgia Bruno, Domenico Ippolito, Vincenzo Pota, Salvatore Dongiovanni, Giacomo Sica, Giorgio Bocchini, Tullio Valente, Mariano Scaglione, Pier Paolo Mainenti and Salvatore Guarino
J. Clin. Med. 2024, 13(11), 3103; https://doi.org/10.3390/jcm13113103 (registering DOI) - 25 May 2024
Abstract
Background: Amyotrophic lateral sclerosis (ALS) is a neuromuscular progressive disorder characterized by limb and bulbar muscle wasting and weakness. A total of 30% of patients present a bulbar onset, while 70% have a spinal outbreak. Respiratory involvement represents one of the worst prognostic [...] Read more.
Background: Amyotrophic lateral sclerosis (ALS) is a neuromuscular progressive disorder characterized by limb and bulbar muscle wasting and weakness. A total of 30% of patients present a bulbar onset, while 70% have a spinal outbreak. Respiratory involvement represents one of the worst prognostic factors, and its early identification is fundamental for the early starting of non-invasive ventilation and for the stratification of patients. Due to the lack of biomarkers of early respiratory impairment, we aimed to evaluate the role of chest dynamic MRI in ALS patients. Methods: We enrolled 15 ALS patients and 11 healthy controls. We assessed the revised ALS functional rating scale, spirometry, and chest dynamic MRI. Data were analyzed by using the Mann–Whitney U test and Cox regression analysis. Results: We observed a statistically significant difference in both respiratory parameters and pulmonary measurements at MRI between ALS patients and healthy controls. Moreover, we found a close relationship between pulmonary measurements at MRI and respiratory parameters, which was statistically significant after multivariate analysis. A sub-group analysis including ALS patients without respiratory symptoms and with normal spirometry values revealed the superiority of chest dynamic MRI measurements in detecting signs of early respiratory impairment. Conclusions: Our data suggest the usefulness of chest dynamic MRI, a fast and economically affordable examination, in the evaluation of early respiratory impairment in ALS patients. Full article
(This article belongs to the Section Nuclear Medicine & Radiology)
14 pages, 251 KiB  
Article
Mapping Developmental Assets in Kosovo’s Youth: Insights into the Internal Cohesion Psychotherapeutic Process for Young People Experiencing Anxiety and Depression: A Qualitative Study
by Fatime Rrahmani, Veronë Perçuku, Viola Greiçevci, Arta Rexhepi and Fitim Uka
Youth 2024, 4(2), 745-758; https://doi.org/10.3390/youth4020050 (registering DOI) - 25 May 2024
Abstract
This study explores anxiety and depression among young individuals in Kosovo, considering socio-political and economic influences. Introducing Positive Youth Development (PYD) as a framework, the study explores its integration with Internal Cohesion Psychotherapy (ICP) to address youth mental health challenges. Empirical evidence underscores [...] Read more.
This study explores anxiety and depression among young individuals in Kosovo, considering socio-political and economic influences. Introducing Positive Youth Development (PYD) as a framework, the study explores its integration with Internal Cohesion Psychotherapy (ICP) to address youth mental health challenges. Empirical evidence underscores ICP’s positive impact on mental health outcomes, emphasizing developmental assets. Thematic analysis of participant narratives reveals six key themes, showcasing ICP’s transformative nature, the critical role of social support networks, values and beliefs, reflections on personal growth, and therapy’s extended impact on relationships. Participants’ stories highlight ICP’s profound influence on personal growth, social relationships, and mental health. The study examines internal and external assets, emphasizing social networks, values, beliefs, and cultural competence. Insights into ICP’s intrapersonal and professional systems reveal developmental assets fostering growth, motivation, and engagement. External assets like empowerment, safety, and service to others, alongside internal assets like commitment to learning, positive values, integrity, and responsibility, contribute to an understanding of factors influencing Kosovo’s young population. In conclusion, the integration of PYD and ICP offers a powerful synergy to address mental health challenges, providing valuable insights into specific developmental assets crucial for optimizing interventions and promoting mental health among young Kosovars. Full article
17 pages, 3238 KiB  
Article
Application of Procyanidins from Aronia melanocarpa (Michx.) Elliott in Fresh-Cut Apple Preservation
by Shangjian Li, Jiajia Chen, Sarengaowa, Chen Chen and Wenzhong Hu
Horticulturae 2024, 10(6), 556; https://doi.org/10.3390/horticulturae10060556 (registering DOI) - 25 May 2024
Abstract
The aim of this study was to find a purely natural and effective preservative that could be used in fresh-cut apples extracting proanthocyanidin (PC) components from black chokeberry (Aronia melanocarpa (Michx.) Elliott) using DESs (deep eutectic solvents). DM130 microporous resins were used [...] Read more.
The aim of this study was to find a purely natural and effective preservative that could be used in fresh-cut apples extracting proanthocyanidin (PC) components from black chokeberry (Aronia melanocarpa (Michx.) Elliott) using DESs (deep eutectic solvents). DM130 microporous resins were used in purification experiments to obtain proanthocyanidin purifiers with a higher content. To investigate the free radical scavenging ability (DPPH, ·OH, O2, ABTS) of the resulting proanthocyanidin purifiers, experiments were conducted due to their potent antioxidant properties. The results show that the polyphenol extract from black chokeberry demonstrated an excellent antioxidant capacity, with more than 95% scavenging of DPPH, ABTS and superoxide anion at concentrations above 0.1 mg/mL. Furthermore, the antibacterial efficacy of the product was also evaluated. The results of this study on the inhibitory ability of bacteria demonstrated that PC exhibited superior inhibitory efficacy against E. coli and S. aureus, with inhibitory circles of 16 ± 0.11 mm and 18 ± 0.09 mm, respectively. Conversely, PC demonstrated no inhibitory efficacy against A. niger and Penicillium sp. Finally, the effect of PC in preserving fresh-cut apples was tested in terms of weight loss, hardness, appearance and total number of microorganisms. The results demonstrated a 26.44% reduction in weight loss and a 13.5% increase in hardness, as well as a 98% reduction in total bacterial counts in apple pieces treated with 5 mg/mL of PC in comparison to those left untreated. Full article
(This article belongs to the Special Issue Postharvest Application of Edible Coatings on Fruits and Vegetables)
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16 pages, 559 KiB  
Review
Challenges of Revisional Metabolic and Bariatric Surgery: A Comprehensive Guide to Unraveling the Complexities and Solutions of Revisional Bariatric Procedures
by Lorna A. Evans, Rocio Castillo-Larios, Jorge Cornejo and Enrique F. Elli
J. Clin. Med. 2024, 13(11), 3104; https://doi.org/10.3390/jcm13113104 (registering DOI) - 25 May 2024
Abstract
Revisional metabolic and bariatric surgery (RMBS) presents unique challenges in addressing weight loss failure or complications arising from initial bariatric procedures. This review aims to explore the complexities and solutions associated with revisional bariatric procedures comprehensively, offering insights into the evolving terrain of [...] Read more.
Revisional metabolic and bariatric surgery (RMBS) presents unique challenges in addressing weight loss failure or complications arising from initial bariatric procedures. This review aims to explore the complexities and solutions associated with revisional bariatric procedures comprehensively, offering insights into the evolving terrain of metabolic and bariatric surgery. A literature review is conducted to identify pertinent studies and expert opinions regarding RMBS. Methodological approaches, patient selection criteria, surgical techniques, preoperative assessments, and postoperative management strategies are synthesized to provide a comprehensive overview of current practices and advancements in the field, including institutional protocols. This review synthesizes key findings regarding the challenges encountered in RMBS, including the underlying causes of primary procedure failure, anatomical complexities, technical considerations, and assessments of surgical outcomes. Additionally, patient outcomes, complication rates, and long-term success are presented, along with institutional approaches to patient assessment and procedure selection. This review provides valuable insights for clinicians grappling with the complexities of RMBS. A comprehensive understanding of patient selection, surgical techniques, preoperative management, and postoperative care is crucial for enhancing outcomes and ensuring patient satisfaction in the field of metabolic bariatric surgery. Full article
(This article belongs to the Special Issue Robotic Bariatric Surgery)
19 pages, 3276 KiB  
Article
Machine Learning Model in Obesity to Predict Weight Loss One Year after Bariatric Surgery: A Pilot Study
by Enrique Nadal, Esther Benito, Ana María Ródenas-Navarro, Ana Palanca, Sergio Martinez-Hervas, Miguel Civera, Joaquín Ortega, Blanca Alabadi, Laura Piqueras, Juan José Ródenas and José T. Real
Biomedicines 2024, 12(6), 1175; https://doi.org/10.3390/biomedicines12061175 (registering DOI) - 25 May 2024
Abstract
Roux-en-Y gastric bypass (RYGB) is a treatment for severe obesity. However, many patients have insufficient total weight loss (TWL) after RYGB. Although multiple factors have been involved, their influence is incompletely known. The aim of this exploratory study was to evaluate the feasibility [...] Read more.
Roux-en-Y gastric bypass (RYGB) is a treatment for severe obesity. However, many patients have insufficient total weight loss (TWL) after RYGB. Although multiple factors have been involved, their influence is incompletely known. The aim of this exploratory study was to evaluate the feasibility and reliability of the use of machine learning (ML) techniques to estimate the success in weight loss after RYGP, based on clinical, anthropometric and biochemical data, in order to identify morbidly obese patients with poor weight responses. We retrospectively analyzed 118 patients, who underwent RYGB at the Hospital Clínico Universitario of Valencia (Spain) between 2013 and 2017. We applied a ML approach using local linear embedding (LLE) as a tool for the evaluation and classification of the main parameters in conjunction with evolutionary algorithms for the optimization and adjustment of the parameter model. The variables associated with one-year postoperative %TWL were obstructive sleep apnea, osteoarthritis, insulin treatment, preoperative weight, insulin resistance index, apolipoprotein A, uric acid, complement component 3, and vitamin B12. The model correctly classified 71.4% of subjects with TWL < 30% although 36.4% with TWL ≥ 30% were incorrectly classified as “unsuccessful procedures”. The ML-model processed moderate discriminatory precision in the validation set. Thus, in severe obesity, ML-models can be useful to assist in the selection of patients before bariatric surgery. Full article
(This article belongs to the Special Issue Artificial Intelligence Applications in Cancer and Other Diseases)
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17 pages, 5500 KiB  
Article
Behavioral Effects of the Mixture and the Single Compounds Carbendazim, Fipronil, and Sulfentrazone on Zebrafish (Danio rerio) Larvae
by Samara da Silva Gomes, Jadson Freitas da Silva, Renata Meireles Oliveira Padilha, João Victor Alves de Vasconcelos, Luís Gomes de Negreiros Neto, James A. Marrs and Pabyton Gonçalves Cadena
Biomedicines 2024, 12(6), 1176; https://doi.org/10.3390/biomedicines12061176 (registering DOI) - 25 May 2024
Abstract
Pesticides are often detected in freshwater, but their impact on the aquatic environment is commonly studied based on single compounds, underestimating the potential additive effects of these mixtures. Even at low concentrations, pesticides can negatively affect organisms, altering important behaviors that can have [...] Read more.
Pesticides are often detected in freshwater, but their impact on the aquatic environment is commonly studied based on single compounds, underestimating the potential additive effects of these mixtures. Even at low concentrations, pesticides can negatively affect organisms, altering important behaviors that can have repercussions at the population level. This study used a multi-behavioral approach to evaluate the effects of zebrafish larvae exposure to carbendazim (C), fipronil (F), and sulfentrazone (S), individually and mixed. Five behavioral tests, thigmotaxis, touch sensitivity, optomotor response, bouncing ball test, and larval exploratory behavior, were performed to assess potential effects on anxiety, fear, and spatial and social interaction. Significant changes were observed in the performance of larvae exposed to all compounds and their mixtures. Among the single pesticides, exposure to S produced the most behavioral alterations, followed by F and C, respectively. A synergistic effect between the compounds was observed in the C + F group, which showed more behavioral effects than the groups exposed to pesticides individually. The use of behavioral tests to evaluate pesticide mixtures is important to standardize methods and associate behavioral changes with ecologically relevant events, thus creating a more realistic scenario for investigating the potential environmental impacts of these compounds. Full article
(This article belongs to the Special Issue Zebrafish Models for Development and Disease 4.0)
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20 pages, 5557 KiB  
Article
Strategy for Ensuring the Metrological Traceability of Nanoparticle Size Measurements by SEM
by Nicolas Feltin, Alexandra Delvallée and Loïc Crouzier
Nanomaterials 2024, 14(11), 931; https://doi.org/10.3390/nano14110931 (registering DOI) - 25 May 2024
Abstract
The concept of measurement traceability is crucial for ensuring the data reliability and the comparability of measurement results provided by different instruments and operators. In the field of nanoparticle metrology, determining the size of nanoparticles using electron microscopy-based techniques remains a real challenge. [...] Read more.
The concept of measurement traceability is crucial for ensuring the data reliability and the comparability of measurement results provided by different instruments and operators. In the field of nanoparticle metrology, determining the size of nanoparticles using electron microscopy-based techniques remains a real challenge. In laboratory settings, the establishment of traceability regarding the instrument calibration procedures, the assessment of uncertainties associated with instruments/operators/samples/environments, as well as the complexities related to electron–sample interactions, are often neglected. In this article, we describe the calibration procedure set up at the LNE (Laboratoire National de métrologie et d’Essais) and propose an evaluation method for determining the uncertainties in measuring nanoparticle size by SEM (Scanning Electron Microscopy). This study investigates the impact of the energy of the primary electrons (PEs) generated by the electron beam and accelerating voltage on the reliability of size measurements. The convolution between the signals coming from a nanoparticle and the substrate on which the particle is deposited induces edge effects that can have a negative impact on the measurement results. Finally, a diagram describing the various stages involved in establishing traceability for SEM measurements of nanoparticle size is proposed to facilitate the work of future operators. Full article
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26 pages, 1881 KiB  
Review
A Review of Forest Ecosystem Services and Their Spatial Value Characteristics
by Carl Nolander and Robert Lundmark
Forests 2024, 15(6), 919; https://doi.org/10.3390/f15060919 (registering DOI) - 25 May 2024
Abstract
Forests provide a variety of resources and benefits, but only a few, such as timber, are traded on the markets. Ecosystem service valuation is a method for quantifying the non-market benefits of forests to understand the full costs of forest management. This review [...] Read more.
Forests provide a variety of resources and benefits, but only a few, such as timber, are traded on the markets. Ecosystem service valuation is a method for quantifying the non-market benefits of forests to understand the full costs of forest management. This review examines the forest ecosystem service valuations over the past 20 years, with a particular focus on their spatial modeling. The literature review method is designed to provide a systematic, explicit, and reproducible outcome concerning the valuations of forest ecosystem services and the contextual setting of these valuations. The findings suggest that there is a huge variation in the values reported for similar ecosystem services but that carbon sequestration, recreation in forests, and hydrological services, such as watershed protection and flood prevention, are the ecosystem services that are consistently valued highly in the reviewed studies. In the last ten years, studies have more frequently modeled ecosystem services in spatial terms. Full article
(This article belongs to the Special Issue Economic Valuation of Forest Resources)
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17 pages, 3448 KiB  
Article
The microRNA Let-7f Induces Senescence and Exacerbates Oxidative Stress in Retinal Pigment Epithelial Cells
by Christina Ortiz, Houda Tahiri, Chun Yang, Claudia Gilbert, Carl Fortin and Pierre Hardy
Antioxidants 2024, 13(6), 646; https://doi.org/10.3390/antiox13060646 (registering DOI) - 25 May 2024
Abstract
This study aims to investigate the role of microRNA let-7f in the dysfunction and degeneration of retinal pigment epithelium (RPE) cells through the induction of senescence and oxidative stress. Furthermore, we explore whether let-7f inhibition can protect these cells against sodium iodate (SI)-induced [...] Read more.
This study aims to investigate the role of microRNA let-7f in the dysfunction and degeneration of retinal pigment epithelium (RPE) cells through the induction of senescence and oxidative stress. Furthermore, we explore whether let-7f inhibition can protect these cells against sodium iodate (SI)-induced oxidative stress. Oxidative stress and let-7f expression are reciprocally regulated in retinal pigment epithelial cells. Overexpression of let-7f in ARPE-19 cells induced oxidative stress as demonstrated by increased reactive oxygen species (ROS) production as well as senescence. Inhibition of let-7f successfully protected RPE cells from the detrimental effects induced by SI. In addition, let-7f overexpression induced RPE cellular dysfunction by diminishing their migratory capabilities and reducing the phagocytosis of porcine photoreceptor outer segments (POS). Results were further confirmed in vivo by intravitreal injections of SI and let-7f antagomir in C57BL/6 mice. Our results provide strong evidence that let-7f is implicated in the dysfunction of RPE cells through the induction of senescence and oxidative injury. These findings may help to uncover novel and relevant processes in the pathogenesis of dry AMD. Full article
14 pages, 250 KiB  
Article
Characterisation of Pasteurella multocida Strains from Different Lesions in Rabbits
by Francesco D’Amico, Davide Messina, Gaia Casalino, Michele Schiavitto, Antonella Bove, Diana Romito, Francesco Paolo D’Onghia, Antonio Camarda and Elena Circella
Animals 2024, 14(11), 1569; https://doi.org/10.3390/ani14111569 (registering DOI) - 25 May 2024
Abstract
Pasteurellosis, a disease caused by Pasteurella multocida, is responsible for economic losses in rabbit industrial farms due to rhinitis, conjunctivitis, pneumonia, metritis, mastitis, orchitis, subcutaneous abscesses, otitis, encephalitis, and septicaemic forms. Although the occurrence of the disease is conditioned by predisposing factors [...] Read more.
Pasteurellosis, a disease caused by Pasteurella multocida, is responsible for economic losses in rabbit industrial farms due to rhinitis, conjunctivitis, pneumonia, metritis, mastitis, orchitis, subcutaneous abscesses, otitis, encephalitis, and septicaemic forms. Although the occurrence of the disease is conditioned by predisposing factors that affect the rabbit immune response, the strains of P. multocida involved in the infection may have a different pathogenic ability. Therefore, typing of strains spread among the rabbits is important to assess their pathogenic potential. The aim of this study is to investigate the P. multocida strains responsible for disease in rabbit industrial farms. A total of 114 strains identified from different lesions were serotyped. Additionally, the presence of virulence-associated genes was investigated using three PCR (polymerase chain reaction) protocols. Capsular type A was prevalently found in strains from respiratory lesions while types D and F in those from metritis, mastitis, and other lesions. Different associations between some virulence-associated genes and both capsular type and lesions found in rabbits were detected. The presence of 8 virulence-associated genes seems to increase the occurrence of metritis. In addition, strains belonging to capsular type A and responsible for respiratory disorders especially, were found equipped with 10 and 11 virulence-associated genes. Nevertheless, the presence of strains responsible only for rhinitis was also detected among the latter, suggesting that the pathogenic ability of the bacteria depends on the expression rather than the presence of a gene. Full article
(This article belongs to the Special Issue Infectious Diseases, Pathology and Immunology in Rabbits)
12 pages, 1606 KiB  
Article
Comparison of Sub-Ppm Instrument Response Suggests Higher Detection Limits Could Be Used to Quantify Methane Emissions from Oil and Gas Infrastructure
by Stuart N. Riddick, Mercy Mbua, Ryan Brouwer, Ethan W. Emerson, Abhinav Anand, Elijah Kiplimo, Seunfunmi Ojomu, Jui-Hsiang Lo and Daniel J. Zimmerle
Sensors 2024, 24(11), 3407; https://doi.org/10.3390/s24113407 (registering DOI) - 25 May 2024
Abstract
Quantifying and controlling fugitive methane emissions from oil and gas facilities remains essential for addressing climate goals, but the costs associated with monitoring millions of production sites remain prohibitively expensive. Current thinking, supported by measurement and simple dispersion modelling, assumes single-digit parts-per-million instrumentation [...] Read more.
Quantifying and controlling fugitive methane emissions from oil and gas facilities remains essential for addressing climate goals, but the costs associated with monitoring millions of production sites remain prohibitively expensive. Current thinking, supported by measurement and simple dispersion modelling, assumes single-digit parts-per-million instrumentation is required. To investigate instrument response, the inlets of three trace-methane (sub-ppm) analyzers were collocated on a facility designed to release gas of known composition at known flow rates between 0.4 and 5.2 kg CH4 h−1 from simulated oil and gas infrastructure. Methane mixing ratios were measured by each instrument at 1 Hertz resolution over nine hours. While mixing ratios reported by a cavity ring-down spectrometer (CRDS)-based instrument were on average 10.0 ppm (range 1.8 to 83 ppm), a mid-infrared laser absorption spectroscopy (MIRA)-based instrument reported short-lived mixing ratios far larger than expected (range 1.8 to 779 ppm) with a similar nine-hour average to the CRDS (10.1 ppm). We suggest the peaks detected by the MIRA are likely caused by a micrometeorological phenomenon, where vortex shedding has resulted in heterogeneous methane plumes which only the MIRA can observe. Further analysis suggests an instrument like the MIRA (an optical-cavity-based instrument with cavity size ≤10 cm3 measuring at ≥2 Hz with air flow rates in the order of ≤0.3 slpm at distances of ≤20 m from the source) but with a higher detection limit (25 ppm) could detect enough of the high-concentration events to generate representative 20 min-average methane mixing ratios. Even though development of a lower-cost, high-precision, high-accuracy instrument with a 25 ppm detection threshold remains a significant problem, this has implications for the use of instrumentation with higher detection thresholds, resulting in the reduction in cost to measure methane emissions and providing a mechanism for the widespread deployment of effective leak detection and repair programs for all oil and gas infrastructure. Full article
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12 pages, 1347 KiB  
Article
Strength and Contaminant Toxicity Leaching Characteristics of MgO-Solidified Silt
by Shi Shu, Xiaohuan Zhou, Yujie Gong, Haohui Wang, Yan Tang and Junhao Chen
Processes 2024, 12(6), 1086; https://doi.org/10.3390/pr12061086 (registering DOI) - 25 May 2024
Abstract
Abstract: In this study, MgO as an environmentally friendly silt-solidifying material was first mixed with silt and then carbonized by injection with CO2. The strength and contaminant leaching characteristics of the MgO-solidified silt were studied using unconfined compressive [...] Read more.
Abstract: In this study, MgO as an environmentally friendly silt-solidifying material was first mixed with silt and then carbonized by injection with CO2. The strength and contaminant leaching characteristics of the MgO-solidified silt were studied using unconfined compressive strength and toxicity leaching tests, and the results were compared with those of cement-solidified silt. The unconfined compressive strength of the silt reached 111 kPa with 9% MgO content and a 14 d curing time. The CO2 injection further increased the unconfined compressive strength of the MgO-solidified silt by approximately 25%: the values for MgO-solidified silts without and with a CO2 injection were approximately 60% and 80%, respectively, of those of the cement-solidified silts with the same additive additions. The leaching concentrations of nutrient salts and heavy metal pollutants in the silt decreased with increased MgO content. Compared with the dredged silt, MgO solidification with carbonization reduced the leaching of total nitrogen and total phosphorus by more than 10% and 50%, respectively: these values were approximately 5% points higher than those of cement-solidified silt. Of the heavy metals, the leaching concentration of Ni was reduced the most. This study provides a theoretical basis and technical support for low-carbon treatment and green resource utilization of dredging silt. Full article
(This article belongs to the Section Environmental and Green Processes)
22 pages, 11470 KiB  
Article
The Impact of Different Ventilation Conditions on Electric Bus Fires
by Haowei Yao, Mengyang Xing, Huaitao Song, Yang Zhang, Sheng Luo and Zhenpeng Bai
Fire 2024, 7(6), 182; https://doi.org/10.3390/fire7060182 (registering DOI) - 25 May 2024
Abstract
Once a fire breaks out in an electric bus, it can easily lead to mass casualties and severe injuries, resulting in significant property damage and social impact. The high-temperature smoke and toxic gases in an electric bus fire are key factors that cause [...] Read more.
Once a fire breaks out in an electric bus, it can easily lead to mass casualties and severe injuries, resulting in significant property damage and social impact. The high-temperature smoke and toxic gases in an electric bus fire are key factors that cause a large number of casualties, both of which are closely related to ventilation conditions. In view of this, this study utilized the Fire Dynamics Simulator (FDS 6) software to establish a three-dimensional experimental model of an electric bus. Numerical simulations of the fire combustion process in the electric bus under different ventilation conditions were conducted. Multiple fire scenes were established based on varying ventilation areas, different wind speeds, and diverse window opening positions. This study specifically analyzed the temperature and CO concentration variations under different fire scenes. By comparing the simulation results under different ventilation conditions, it can be concluded that when an electric bus catches fire, opening 100% of the windows, the wind speed is 8 m/s, and opening the rear window of the electric bus first can minimize the fire risk. Through the numerical simulation of electric bus fires under various conditions, this study analyzed the impact of different ventilation conditions on electric bus fires, providing a theoretical basis for firefighting and rescue efforts as well as personnel evacuation in electric bus fire incidents, with the ultimate goal of maximizing public safety. Full article
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13 pages, 5584 KiB  
Article
Description of Limb Anomalies Resulting from Molt Irregularities in Ammothea hilgendorfi (Pycnogonida: Ammotheidae)
by Antoine Flandroit, Louis Simon and Guillaume Caulier
Arthropoda 2024, 2(2), 156-168; https://doi.org/10.3390/arthropoda2020012 (registering DOI) - 25 May 2024
Abstract
Limb anomalies are widespread and diversified in arthropods. From trilobites to insects, they range from the loss to the addition or fusion of legs and may appear congenitally or be induced experimentally (e.g., amputation or injury). Basal chelicerates pycnogonids, or sea spiders, also [...] Read more.
Limb anomalies are widespread and diversified in arthropods. From trilobites to insects, they range from the loss to the addition or fusion of legs and may appear congenitally or be induced experimentally (e.g., amputation or injury). Basal chelicerates pycnogonids, or sea spiders, also show deformities. Despite being understudied compared to other arthropods, quite a high diversity of limb malformations has been reported in the literature. The present study reports the leg anomalies of two adult females Ammothea hilgendorfi (Böhm, 1879) observed with duplicated leg podomeres. Both individuals were described ethologically and morphologically. Although the current knowledge on pycnogonids is limited, the anomaly is likely due to a problem in the molting process; the specimens were unable to totally remove their old exuviae, which then stacked after the proximal leg segments. The second specimen also showed other leg deformities, hinting at a problem during the molting process itself. The discussion emphasizes that understanding how pycnogonids normally molt would not only help our understanding of how the abnormal patterns appeared but also put pycnogonids into perspective with other arthropods, a phylum in which they have a key taxonomic position. Full article
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