Modular supramolecular complexes, where different proteins are assembled to gather targeting capability and photofunctional properties within the same structures, are of special interest for bacterial photodynamic inactivation, given their inherent biocompatibility and flexibility. We have recently proposed one such structure, exploiting the tetrameric bacterial protein streptavidin as the main building block, to target S. aureus protein A. To expand the palette of targets, we have linked biotinylated Concanavalin A, a sugar-binding protein, to a methylene blue-labelled streptavidin. By applying a combination of spectroscopy and microscopy, we demonstrate the binding of Concanavalin A to the walls of Gram-positive S. aureus and Gram-negative E. coli. Photoinactivation is observed for both bacterial strains in the low micromolar range, although the moderate affinity for the molecular targets and the low singlet oxygen yields limit the overall efficiency. Finally, we apply a maximum entropy method to the analysis of autocorrelation traces, which proves particularly useful when interpreting signals measured for diffusing systems heterogeneous in size, such as fluorescent species bound to bacteria. Full article

The β-cell workload increases in the setting of insulin resistance and reduced β-cell mass, which occurs in type 2 and type 1 diabetes, respectively. The prolonged elevation of insulin production and secretion during the pathogenesis of diabetes results in β-cell ER stress. The depletion of β-cell Ca²⁺_ER during ER stress activates the unfolded protein response, leading to β-cell dysfunction. Ca²⁺_ER is involved in many pathways that are critical to β-cell function, such as protein processing, tuning organelle and cytosolic Ca²⁺ handling, and modulating lipid homeostasis. Mutations that promote β-cell ER stress and deplete Ca²⁺_ER stores are associated with or cause diabetes (e.g., mutations in ryanodine receptors and insulin). Thus, improving β-cell Ca²⁺_ER handling and reducing ER stress under diabetogenic conditions could preserve β-cell function and delay or prevent the onset of diabetes. This review focuses on how mechanisms that control β-cell Ca²⁺_ER are perturbed during the pathogenesis of diabetes and contribute to β-cell failure. Full article

Green innovation has become a key strategy for reducing emissions. However, existing research mainly examines this phenomenon through the resource and institutional perspectives, often ignoring the changes in corporate green innovation behavior under industry peer pressure. Therefore, this study draws on the policy framework of China’s carbon trading pilot and uses a multi-period difference-in-difference (DID) fixed effects model to explore how carbon trading shapes enterprises’ green innovation strategies. The survey used data from pilot enterprises from 2008 to 2019 and found that carbon trading policies are conducive to green innovation, and both exploratory green innovation and exploitative green innovation have been reflected. It is worth noting that under the influence of peer pressure, this positive effect is more prominent in exploratory green innovation. Furthermore, it was found that firms facing carbon pressure can skillfully find an equilibrium between exploratory green innovation and exploitative green innovation. The research results demonstrate the green innovation strategies and trade-offs of Chinese enterprises facing the impact of carbon trading policies, with the hope that the research conclusions will have certain theoretical reference significance for future corporate green transformation and increased investment in green innovation. Full article

Digital elevation models (DEMs) are widely used in digital terrain analysis, global change research, digital Earth applications, and studies concerning natural disasters. In this investigation, a thorough examination and comparison of five open-source DEMs (ALOS PALSAR, SRTM1 DEM, SRTM3 DEM, NASADEM, and ASTER GDEM V3) was carried out, with a focus on the Chongqing region as a specific case study. By utilizing ICESat-2 ATL08 data for validation and employing a random forest model to refine terrain variables such as slope, aspect, land cover, and landform type, a study was undertaken to assess the precision of DEM data. Research indicates that spatial resolution significantly impacts the accuracy of DEMs. ALOS PALSAR demonstrated satisfactory performance, reducing the corrected root mean square error (RMSE) from 13.29 m to 9.15 m. The implementation of the random forest model resulted in a significant improvement in the accuracy of the 30 m resolution NASADEM product. This improvement was supported by a decrease in the RMSE from 38.24 m to 9.77 m, demonstrating a significant 74.45% enhancement in accuracy. Consequently, the ALOS PALSAR and NASADEM datasets are considered the preferred data sources for mountainous urban areas. Furthermore, the study established a clear relationship between the precision of DEMs and slope, demonstrating a consistent decline in precision as slope steepness increases. The influence of aspect on accuracy was considered to be relatively minor, while vegetated areas and medium-to-high-relief mountainous terrains were identified as the main challenges in attaining accuracy in the DEMs. This study offers valuable insights into selecting DEM datasets for complex terrains in mountainous urban areas, highlighting the critical importance of choosing the appropriate DEM data for scientific research. Full article

Encapsulins are self–assembling nano–compartments that naturally occur in bacteria and archaea. These nano–compartments encapsulate cargo proteins that bind to the shell’s interior through specific recognition sequences and perform various metabolic processes. Encapsulation enables organisms to perform chemical reactions without exposing the rest of the cell to potentially harmful substances while shielding cargo molecules from degradation and other adverse effects of the surrounding environment. One particular type of cargo protein, the ferritin–like protein (FLP), is the focus of this review. Encapsulated FLPs are members of the ferritin–like protein superfamily, and they play a crucial role in converting ferrous iron (Fe⁺²) to ferric iron (Fe⁺³), which is then stored inside the encapsulin in mineralized form. As such, FLPs regulate iron homeostasis and protect organisms against oxidative stress. Recent studies have demonstrated that FLPs have tremendous potential as biosensors and bioreactors because of their ability to catalyze the oxidation of ferrous iron with high specificity and efficiency. Moreover, they have been investigated as potential targets for therapeutic intervention in cancer drug development and bacterial pathogenesis. Further research will likely lead to new insights and applications for these remarkable proteins in biomedicine and biotechnology. Full article

Effectively enhancing oil recovery can be achieved by reducing the viscosity of crude oil. Therefore, this paper investigated the viscosity reduction behavior of carbon nanotube viscosity reducers with different molecular structures at the oil–water interface, aiming to guide the synthesis of efficient viscosity reducers based on molecular structure. This study selected carbon nanotubes with different functional groups (NH₂-CNT, OH-CNT, and COOH-CNT) for research, and carbon nanotubes with varying carbon chain lengths were synthesized. These were then combined with Tween 80 to form a nanofluid. Scanning electron microscopy analysis revealed an increased dispersibility of carbon nanotubes after introducing carbon chains. Contact angle experiments demonstrated that -COOH exhibited the best hydrophilic effect. The experiments of zeta potential, conductivity, viscosity reduction, and interfacial tension showed that, under the same carbon chain length, the conductivity and viscosity reduction rate sequence for different functional groups was -NH₂ < -OH < -COOH. The dispersing and stabilizing ability and interfacial tension reduction sequence for different functional groups was -COOH < -OH < -NH₂. With increasing carbon chain length, conductivity and interfacial tension decreased, and the viscosity reduction rate and the dispersing and stabilizing ability increased. Molecular dynamics simulations revealed that, under the same carbon chain length, the diffusion coefficient sequence for different functional groups was -NH₂ < -OH < -COOH. The diffusion coefficient gradually decreased as the carbon chain length increased, resulting in better adsorption at the oil–water interface. This study holds significant importance in guiding viscosity reduction in heavy oil to enhance oil recovery. Full article

The study presents a novel meshed horizontal–vertical (H–V) geogrid, offering promising advancements in geotechnical structure performance. The study pioneers a modeling approach for H–V geogrid foundation bearing capacity with discrete element method, expanding understanding and optimizing design strategy. By analyzing the granular displacement, contact force distribution, and vertical stress distribution within the foundation system, the study examines the impact of burial depth, vertical element height, and the number of vertical elements on H–V reinforced foundations. The findings suggest that employing a burial depth equivalent to the width of the footing enhances bearing capacity compared to conventional geogrid applications, with depths set at 0.4 times the width of the footing. This enhancement is attributed to forming a deeper slip surface in H–V systems. Moreover, raising vertical elements to 0.6 times the width of the footing enhances bearing capacity with minimal increase in geogrid usage, indicating a strategic approach to reinforcement. Increasing the number of vertical elements, particularly with three pairs, significantly enhances bearing capacity by reinforcing lateral restraint on the soil and promoting stress homogenization, thereby augmenting the “deep-footing” effect. The technical analysis underscores the efficacy of H–V geogrids in bolstering the bearing capacity of reinforced foundations, which is attributed to the robust grip and interlocking mechanism facilitated by these geogrids’ vertical ribs and mesh structure, which augment lateral confinement and diminish horizontal soil displacement. Full article

The diel vertical migration of mesopelagic fishes in the southern (S-May station and S-Nov station) and central (C-Jun station and C-Dec station) South China Sea was investigated through a series of continuous field surveys conducted in May, June, November, and December 2017. These surveys employed a combination of mid-water trawl and acoustics techniques. The diel migration process, vertical distribution, acoustic migration proportion, and migration pattern of mesopelagic fishes were analyzed. The results revealed that mesopelagic fishes initiated an upward migration before sunset, with the process concluding within 30–120 min after dark. Subsequently, they commenced a downward migration before dawn, which terminated within 10–50 min after sunrise. The mesopelagic deep-sea layers of mesopelagic fishes at the S-May, S-Nov, C-Jun, and C-Dec stations ranged from 360 to 700 m, 350 to 680 m, 350 to 520 m, and 300 to 700 m, respectively. The acoustic migration proportions of mesopelagic fishes at the corresponding stations were found to be approximately 44.5%, 25.7%, 29.8%, and 58.0%, respectively. There were seasonal and regional differences in the vertical migration and distribution patterns of mesopelagic fishes in the South China Sea. A total of 228 species were identified, including 203 fish species, 23 cephalopod species, and 2 shark species. Among these, a subset of 43 fish and cephalopod species exhibited extensive diel vertical migrating behavior. Specifically, this subset comprised 23 lanternfish species, 8 cephalopod species, and 12 other fish species. Lanternfishes were the predominant diel vertical migratory species, while cephalopods also played a significant role in diel vertical migration. The diel migration behavior was found to be slight for Diaphus lucidus, Melamphaes microps, Argyropelecus affinis, and six other fish species. Non-migratory behavior was observed in Sternoptyx obscura, Argyropelecus sladeni, Sternoptyx diaphana, and 13 other fish species. The diel migration habits of 178 additional species of fish, cephalopods, and sharks could not be definitively determined. Full article

The characteristics of autonomous underwater vehicles include nonlinearity, strong coupling, multiple inputs and multiple outputs, uncertainty, strong disturbance, underdrive, and multiple constraints. Autonomous underwater vehicle cluster systems are associated with large-scale complex dynamic systems through local perception or network communication, which have the structural characteristics of “complex dynamic + association topology + interaction rules”. To solve the problem of formation trajectory tracking of underactuated autonomous underwater vehicles, a controller was designed on the basis of an improved nonlinear backstepping algorithm, cascade system theory, and the Lyapunov direct method. In this design, the formation is determined from the actual trajectory of the leader autonomous underwater vehicle. The formation control rate is determined using the backstepping method and Lyapunov theory. Nonlinear disturbance observers were added to ensure that the trajectory error of the formation control could be quickly reduced in a real case with interference. The stability and effectiveness of this method were verified through simulation experiments. The robustness of the control algorithm was verified using two simulation cases, and the simulation results show that the proposed control method can maintain the expected formation. Full article

The primary aim of this literature review is to delineate the key inflammatory cytokines involved in the pathophysiology of pulp inflammation. By elucidating the roles of these cytokines, a deeper comprehension of the distinct stages of inflamed pulp can be attained, thereby facilitating more accurate diagnostic strategies in endodontics. The PRISMA statement and Cochrane handbook were used for the search strategy. The keywords were created based on the review question using the PICO framework. The relevant studies were meticulously assessed according to predefined inclusion and exclusion criteria for this systematic review. A rigorous quality checklist was implemented to evaluate each included study, ensuring scrutiny for both quality and risk-of-bias assessments. The initial pilot search conducted on PubMed, Scopus, Cochrane, and WoS databases yielded 10 pertinent articles. Within these articles, multiple cytokines were identified and discussed as potential candidates for use in endodontic diagnosis, notably including IL-8, IL-6, TNF-α, and IL-2. These cytokines have been highlighted due to their significant roles in the inflammatory processes associated with pulp pathology. The identification of specific inflammatory cytokines holds promise for enhancing endodontic diagnostic procedures and exploring diverse treatment modalities. However, the current body of research in this area remains limited. Further comprehensive studies are warranted to fully elucidate the potential of cytokines in refining diagnostic techniques in endodontics. Full article

Tomato production in the Canary Islands has significantly decreased in recent years due to the presence of parasites and pathogens, poor-quality irrigation water, lack of infrastructure modernization, and increased competition. To address this issue, local varieties with better agro-climatic adaptation and organoleptic characteristics have been cultivated. These varieties show their maximum potential under an agro-ecological cultivation system, where the beneficial micro-organisms of the rhizosphere (in general) and mycorrhizal fungi (in particular) have a positive influence on their development, especially when the plants are subjected to biotic or abiotic stresses. Irrigation water in Canary Islands tomato cultivation comes from groundwater sources with moderate levels of sodium and chlorides or sodium and bicarbonates. This study evaluated the response of mycorrizal plants of the local tomato variety “Manzana Negra” under abiotic stress conditions due to the presence of chlorides and bicarbonates. Two tests were carried out with mycorrhizal and non-mycorrhizal plants. In the first one, 0, 75, and 150 mM NaCl solutions were applied. In the second, the nutrient solution was enriched with sodium bicarbonate at doses of 0, 2.5, 5, 7.5, 10, and 12.5 mM. Presence of native mycorrhizae improved the growth and nutrition of plants affected by irrigation with saline and alkaline water containing chloride and sodium carbonate. Symbiosis produced statistically significant increases in all plant-development-related variables (stem length and diameter; fresh and dry weight) in all bicarbonate concentrations. However, the results with the application of sodium chloride do not seem to indicate a positive interaction in most of the analytical parameters at 150 mM NaCl concentration. The mycorrhizal inoculation with local fungi can be interesting in the production of seedlings of this tomato variety in situations of moderate salinity, especially under bicarbonate stress conditions. Full article

The incidence of ulcerative colitis (UC) has increased globally. As a complex disease, the genetic predisposition for UC could be estimated by the polygenic risk score (PRS), which aggregates the effects of a large number of genetic variants in a single quantity and shows promise in identifying individuals at higher lifetime risk of UC. Here, based on a cohort of 2869 UC cases and 2900 controls with genotype array datasets, we used PRSice-2 to calculate PRS, and systematically analyzed factors that could affect the power of PRS, including GWAS summary statistics, population stratification, and impact of variants. After leveraging a stepwise condition analysis, we eventually established the best PRS model, achieving an AUC of 0.713. Meanwhile, samples in the top 20% of the PRS distribution had a risk of UC more than ten times higher than samples in the lowest 20% (OR = 10.435, 95% CI 8.571–12.703). Our analyses demonstrated that including population-enriched, more disease-associated SNPs and using GWAS summary statistics from similar ethnic background can improve the power of PRS. Strictly following the principle of focusing on one population in all aspects of generating PRS can be a cost-effective way to apply genotype-array-derived PRS to practical risk estimation. Full article

Oxidative stress, an imbalance between reactive oxygen species (ROS) and endogenous antioxidants, plays an important role in the development of neurodegenerative diseases, including Parkinson’s. The human brain is vulnerable to oxidative stress because of the high rate of oxygen that it needs and the high levels of polyunsaturated fatty acids, which are substrates of lipid peroxidation. Natural antioxidants inhibit oxidation and reduce oxidative stress, preventing cancer, inflammation, and neurodegenerative disorders. Furthermore, in the literature, it is reported that antioxidants, due to their possible neuroprotective activity, may offer an interesting option for better symptom management, even Parkinson’s disease (PD). Natural antioxidants are usually found in several foods, such as fruits, vegetables, meat, fish, and oil, and in food wastes, such as seeds, peels, leaves, and skin. They can help the system of endogenous antioxidants, protect or repair cellular components from oxidative stress, and even halt lipid, protein, and DNA damage to neurons. This review will examine the extent of knowledge from the last ten years, about the neuroprotective potential effect of natural antioxidants present in food and food by-products, in in vivo and in vitro PD models. Additionally, this study will demonstrate that the pool of dietary antioxidants may be an important tool in the prevention of PD and an opportunity for cost savings in the public health area. Full article

The agro-pastoral transitional zone in northern China is an important agricultural and pastoral production base. This study focuses on the eastern section of the agro-pastoral transitional zone in northern China. Combined with spatio-temporal analysis, stepwise regression, and gray relation analysis, we analyzed the average annual growth rate, spatio-temporal changes, and influencing factors of agricultural and pastoral production in the study area during 2000–2020. The results show that, in the past 20 years, the agricultural and pastoral production of the 50 municipal districts and counties has significantly changed, among which the agricultural production of 38 municipal districts and counties has shown an extremely significant increase. Generally, the growth rate of agricultural production is higher than that of pastoral production. Agricultural and pastoral production in the study area is influenced by socio-economic and land use/cover factors, with an average correlation degree of 0.79 and 0.88, respectively. Climate change affects agricultural production in agricultural counties, with a correlation degree of 0.85. The results of this research provide valuable insights into understanding the long-term temporal and spatial changes in agricultural and pastoral production and help to develop sound agriculture and pastoral management practices in the eastern section of the agro-pastoral transitional zone in Northern China. Full article

The human microbiome, a complex ecosystem of bacteria, viruses, and protozoans living in symbiosis with the host, plays a crucial role in human health, influencing everything from metabolism to immune function. Dysbiosis, or an imbalance in this ecosystem, has been linked to various health issues, including diabetes and gestational diabetes (GD). In diabetes, dysbiosis affects the function of adipose tissue, leading to the release of adipokines and cytokines, which increase inflammation and insulin resistance. During pregnancy, changes to the microbiome can exacerbate glucose intolerance, a common feature of GD. Over the past years, burgeoning insights into the gut microbiota have unveiled its pivotal role in human health. This article comprehensively reviews literature from the last seven years, highlighting the association between gut microbiota dysbiosis and GD, as well as the metabolism of antidiabetic drugs and the potential influences of diet and probiotics. The underlying pathophysiological mechanisms discussed include the impact of dysbiosis on systemic inflammation and the interplay with genetic and environmental factors. By focusing on recent studies, the importance of considering microbial health in the prevention and treatment of GD is emphasized, providing insights into future research directions and clinical applications to improve maternal–infant health outcomes. Full article

Spatialization and analysis of the gross domestic product of second and tertiary industries (GDP₂₃) can effectively depict the socioeconomic status of regional development. However, existing studies mainly conduct GDP spatialization using nighttime light data; few studies specifically concentrated on the spatialization and analysis of GDP₂₃ in a built-up area by combining multi-source remote sensing images. In this study, the NPP-VIIRS-like dataset and Sentinel-2 multi-spectral remote sensing images in six years were combined to precisely spatialize and analyze the variation patterns of the GDP₂₃ in the built-up area of Zibo city, China. Sentinel-2 images and the random forest (RF) classification method based on PIE-Engine cloud platform were employed to extract built-up areas, in which the NPP-VIIRS-like dataset and comprehensive nighttime light index were used to indicate the nighttime light magnitudes to construct models to spatialize GDP₂₃ and analyze their change patterns during the study period. The results found that (1) the RF classification method can accurately extract the built-up area with an overall accuracy higher than 0.90; the change patterns of built-up areas varied among districts and counties, with Yiyuan county being the only administrative region with an annual expansion rate of more than 1%. (2) The comprehensive nighttime light index is a viable indicator of GDP₂₃ in the built-up area; the fitted model exhibited an R² value of 0.82, and the overall relative errors of simulated GDP₂₃ and statistical GDP₂₃ were below 1%. (3) The year 2018 marked a significant turning point in the trajectory of GDP₂₃ development in the study area; in 2018, Zhoucun district had the largest decrease in GDP₂₃ at −52.36%. (4) GDP₂₃ gradation results found that Zhangdian district exhibited the highest proportion of high GDP₂₃ (>9%), while the proportions of low GDP₂₃ regions in the remaining seven districts and counties all exceeded 60%. The innovation of this study is that the GDP₂₃ in built-up areas were first precisely spatialized and analyzed using the NPP-VIIRS-like dataset and Sentinel-2 images. The findings of this study can serve as references for formulating improved city planning strategies and sustainable development policies. Full article

Studies have suggested that alkalinized foods may reduce the effects of the acidogenic Western diet in promoting obesity, metabolic syndrome, type 2 diabetes, cancer, and coronary heart disease. Indeed, a recent study in mice fed a high-fat diet containing dietary beef supplemented with ammonium hydroxide showed improvement in a suite of metabolic outcomes. However, the effects of dietary protein ammonium supplementation on the microbiome remain unknown. In this study, the effects of ammonium supplementation on beef protein towards microbiome taxa and function in a high-fat diet were analyzed. Fecal microbiomes were characterized using a shotgun metagenomic approach for 16-month-old male and female mice after long-term diet treatments. The results for ammoniated diets showed that several bacteria known to be associated with health benefits increased significantly, including Romboutsia, Oscillospiraceae, and Lactococcus cremoris. The beneficial mucin-degrader Akkermansia was especially abundant, with a high prevalence (~86%) in females. Concurrently, the phyla Actinomycetota (Actinobacteria) and Bacteroidota (Bacteroidetes) were significantly reduced. While sex was a confounding factor affecting microbiome responses to ammonium supplementation in dietary protein, it is worth noting that several putatively beneficial microbiome functions increased with ammonium supplementation, such as glycine betaine transport, xenobiotic detoxification, enhanced defense, and others. Conversely, many disease-associated microbiome functions reduced. Importantly, modifying protein pH alone via ammonium supplementation induced beneficial microbiota changes. Taken together, these results suggest that ammonium-supplemented proteins may mediate some negative microbiome-associated effects of high-fat/Western diets. Full article

Listeria (L.) monocytogenes is an opportunistic foodborne pathogen that causes listeriosis in humans and animals, reaching up to 30% case mortality. There are only a few reports in Mexico about the L. monocytogenes strains found in various foods. The aim of this study was to determine the prevalence of L. monocytogenes, serogroups, virulence genes, and antimicrobial resistance in different foods from Reynosa, Tamaulipas, Mexico. L. monocytogenes strains were characterized by microbiological and molecular methods. Susceptibility to 12 antibiotics was determined according to CLSI and EUCAST. A total of 300 samples of seafood, pasteurized and raw milk, cheese, beef, and chicken were collected from supermarkets and retail markets. The presence of L. monocytogenes was detected in 5.6% of the samples. Most strains belonged to serogroups 4b, 4d, and 4e (68.4%). All strains presented a minimum of four virulence genes; the most common were actA, hly, and plcB (92.1%). A high percentage of antimicrobial susceptibility was observed, with resistance only to STX-TMP (78.9%), STR (26.3%), MEM (21.0%), and E (2.6%). These results show that the foods in Reynosa, Tamaulipas, are a reservoir of L. monocytogenes and represent a potential health risk. Full article

Breast cancer is the most common malignancy in the female sex; although recent therapies have significantly changed the natural history of this cancer, it remains a significant challenge. In the past decade, evidence has been put forward that some oncogenic viruses may play a role in the development of sporadic breast cancer; however, data are scattered and mostly reported as sparse case series or small case–control studies. In this review, we organize and report current evidence regarding the role of high-risk human papillomavirus, mouse mammary tumor virus, Epstein–Barr virus, cytomegalovirus, bovine leukemia virus, human polyomavirus 2, and Merkel cell polyomavirus in the pathogenesis of breast cancer. Full article

Abstract: Slimy sculpin (Uranidea cognata) inhabit coldwater streams in southeastern Minnesota, USA, many of which were subjected to probable 2000-year flood events in August 2007. Floods scoured streambeds, created new stream channels, and greatly reduced benthic invertebrate communities that serve as the primary food resource for sculpin. Diets and Fulton condition of sculpin in Gilmore Creek (with moderate flooding) and Garvin Brook (with very severe flooding) had been examined just prior to flooding and were re-examined 2 weeks after flooding to assess possible diet and condition changes. Diets, body condition, and reproductive fitness of sculpin were examined 7 months post-flood in these same two streams, plus nearby Trout Run (which also experienced very severe flooding). Sculpin condition declined slightly post-flood in Garvin Brook but improved in Gilmore Creek. Prior to spring spawning, the condition of Garvin Brook sculpin had improved, but Gilmore Creek fish condition had worsened. Sculpin diets were more diverse before and after flooding in Gilmore Creek than in Garvin Brook, although the diets of fish from both streams were dominated (>55%) by midge (Diptera: Chironomidae) larvae. Diets remained largely unchanged before versus after flooding in the more severely flooded Garvin Brook, but they changed in Gilmore Creek, becoming more midge-dominated. Prey number per sculpin stomach declined post-flood in Gilmore Creek but not in Garvin Brook, although the dry mass of prey/fish wet mass declined post-flood in both streams. Pre-spawn sculpin displayed no patterns in reproductive fitness (gonadosomatic index, hepatosomatic index, oocyte number) among the three streams that may have been related to flooding severity the previous summer. Sculpin diets and condition were not altered as expected by flooding, and food resource recovery apparently was rapid enough to prevent longer-term impacts on sculpin condition and reproductive fitness in the streams examined. Full article

Suicide is a major public health priority, and its molecular mechanisms appear to be related to glial abnormalities and specific transcriptional changes. This study aimed to identify and synthesize evidence of the relationship between glial dysfunction and suicidal behavior to understand the neurobiology of suicide. As of 26 January 2024, 46 articles that met the inclusion criteria were identified by searching PubMed and ISI Web of Science. Most postmortem studies, including 30 brain regions, have determined no density or number of total Nissl-glial cell changes in suicidal patients with major psychiatric disorders. There were 17 astrocytic, 14 microglial, and 9 oligodendroglial studies using specific markers of each glial cell and further on their specific gene expression. Those studies suggest that astrocytic and oligodendroglial cells lost but activated microglia in suicides with affective disorder, bipolar disorders, major depression disorders, or schizophrenia in comparison with non-suicided patients and non-psychiatric controls. Although the data from previous studies remain complex and cannot fully explain the effects of glial cell dysfunction related to suicidal behaviors, they provide risk directions potentially leading to suicide prevention. Full article

Background: Eyelash extensions (EEs) are among the most popular cosmetic procedures today. There is no prospective study demonstrating how this procedure affects the ocular surface and eye dryness in particular. The goal of this study is to evaluate the effect of EEs removal on dry eye symptoms and signs. Materials and Methods: The subjects were prospectively recruited from routine clinical examinations for dry eye complaints. Only subjects with an OSDI score above 31 were included in the study. The subjects also planned to have the EEs removed and agreed to abstain from makeup use and new cosmetic procedures for 4 weeks. The presence of dry eye was evaluated by an OSDI questionnaire, and objectively by tear breakup time (TBUT), staining (Oxford scale) and blinking intervals. All tests for dry eye were performed at baseline and 4 weeks after EEs removal. Results: The mean age of our patients, all female, was 28 years. The size and type of EEs was diverse. The decision process was mainly based on appearance and models. None of the subjects had any health conditions. The mean result from the score from the OSDI questionnaire at the baseline was 33.4 and improved to 26.7 points 4 weeks after EEs removal. Objectively, the mean TBUT increased from 11.25 to 13.96 s. For the same period, the blinks increased by two per minute, and the staining was reduced by 1.0 grade. Conclusions: Removal of EEs improves the symptoms and the objective signs of dry eye. The most popular beauty procedure regarding eyelashes might not be innocuous to eye health. Full article