Skeletal muscle is composed of bundles of muscle fibers with distinctive characteristics. Oxidative muscle fiber types contain higher mitochondrial content, relying primarily on oxidative phosphorylation for ATP generation. Notably, as a result of obesity, or following prolonged exposure to a high-fat diet, skeletal muscle undergoes a shift in fiber type toward a glycolytic type. Mitochondria are highly dynamic organelles, constantly undergoing mitochondrial biogenesis and dynamic processes. Our study aims to explore the impact of obesity on skeletal muscle mitochondrial biogenesis and dynamics and also ascertain whether the skeletal muscle fiber type shift occurs from the aberrant mitochondrial machinery. Furthermore, we investigated the impact of exercise in preserving the oxidative muscle fiber types despite obesity. Mice were subjected to a normal standard chow and water or high-fat diet with sugar water (HFS) with or without exercise training. After 12 weeks of treatment, the HFS diet resulted in a noteworthy reduction in the markers of mitochondrial content, which was recovered by exercise training. Furthermore, higher mitochondrial biogenesis markers were observed in the exercised group with a subsequent increase in the mitochondrial fission marker. In conclusion, these findings imply a beneficial impact of moderate-intensity exercise on the preservation of oxidative capacity in the muscle of obese mouse models. Full article

Introduction: An estimated 5800 to 46,500 lives are lost due to homelessness each year. Experiencing homelessness and poor health are cyclically related, with one reinforcing the other. Mobile programs, which include vehicles that travel to deliver care, and street medicine, the act of bringing care to spaces where PEH live, may play a role in alleviating this burden by providing trusted, affordable, and accessible care to this community. Methods: We conducted a scoping review of peer-reviewed literature on the role of mobile clinics and street medicine in providing care for PEH by searching PubMed, Embase, and Web of Science on 10 August 2023. Articles from 2013 to 2023 specific to programs in the United States were included. The protocol was developed following the PRISMA-ScR guidelines. The primary outcome was the role of mobile programs for persons experiencing homelessness. Results: A total of 15 articles were included in this review. The descriptive findings emphasized that street medicine and mobile clinics provide primary care, behavioral health, and social services. The utilization findings indicate that street medicine programs positively impact the health system through their ability to defer emergency department and hospital visits, providing financial benefits. The comparative findings between mobile programs and office-based programs indicate current successes and areas for improvement. Discussion: Mobile clinics and street medicine programs that serve PEH provide a wide range of services. While more significant structural change is needed to address healthcare costs and housing policies in the United States, mobile clinics and street medicine teams can improve healthcare access and the healthcare system. Full article

The Zhaotong area in Yunnan Province stands out as one of the most susceptible areas to landslide disasters. The landslide susceptibility of the Zhaotong area can be attributed to its steep terrain, fractured rock formations and strong rainfall, compounded by its frequent seismic activity. This study utilized landslide data provided by the Zhaotong City Natural Resources and Planning Bureau and visually interpreted from high-resolution satellite images of Google Earth to establish the landslide database of the Zhaotong area, including 161 landslides and 3646 potential geological disasters. The distribution characteristics and possible influencing factors of landslides within the Zhaotong area were analyzed using the aforementioned data. The results show that the spatial distribution of landslides and potential geological disasters is roughly consistent; the most concentrated landslides occurred at the junction of Yiliang County, Zhaotong City, and Daguan County, indicating the necessity to enhance surveillance of these landslide-prone areas. The relationship of landslide locations and different influencing factors suggests that elevation, slope angle, and distance to rivers are closely related to landslide occurrence. Landslides are more likely to occur in areas with lower elevations with slope angles ranging from 10° to 40° and near river channels. Full article

This systematic review of 54 cross-disciplinary peer-reviewed causal empirical studies helps public health officials, researchers, and healthcare professionals better comprehend the effects of fear appeals in vaccine promotional campaigns on message processing, persuasion, vaccination attitudes, and vaccination intentions. This review documents inconsistent findings across studies, which it attempts to clarify by considering differences in research designs, sample populations, and outcomes measured. In general, we find that fear appeals increase risk perceptions, message involvement, and vaccination attitudes. However, fear appeals have less influence on vaccination intentions, especially among female and general adult populations or populations from the U.S. and other Western cultures. On the other hand, the effect of fear appeals on vaccination intentions is stronger among student populations and those from China (People’s Republic of China and Hong Kong) and other non-Western cultures. Also, fear appeals are less persuasive when promoting COVID-19 vaccines and boosters than they are for other vaccines (e.g., HPV, influenza, MMR). Future research should compare fear appeal effectiveness in messages across vaccines or when combined with other executional elements, such as the endorser or type of evidence provided. Finally, future studies should explore other methodological approaches and measure underexplored message outcomes, such as vaccine uptake behavior, in more naturalistic settings. Full article

The Tibetan Plateau (TP) serves as a crucial ecological barrier in Asia, with vegetation playing a pivotal role in the terrestrial ecosystem by facilitating energy exchange between the land and atmosphere, regulating climate, and participating in the carbon cycle. In this study, we analyze the characteristics of surface vegetation on the TP in the growing season during 1982–2018 using satellite remote sensing data obtained from the National Oceanic and Atmospheric Administration (NOAA) and China Meteorological Forcing Dataset (CMFD). We investigate how these characteristics respond to climate change under different warming and humidification conditions across the TP. The main conclusions are as follows. (1) The normalized difference vegetation index (NDVI) values on the TP exhibit a gradual decrease from southeast to northwest during the growing season. There is a significant overall increasing trend at a climate tendency rate of 0.01·decade⁻¹ (p < 0.01) from 1982 to 2018, characterized by a notable mutation of around 1998. Over the past 37 years, a polarized trend of vegetation was observed on the TP, with notable improvement in its central and eastern regions. However, there has been noticeable degradation in northwestern TP, specifically within the Kunlun Mountains and Qaidam Basin. (2) The climate of the TP demonstrates distinct regional disparities in terms of warming and humidification characteristics before and after 1998. During the period of 1982–1998 (1998–2018), the temperature increase is primarily concentrated in the northern (southern) TP, while precipitation increase is mainly observed in the southern and northwestern (northeastern and western) regions of the TP. (3) The responses of surface vegetation to climate factors exhibit significant variations across diverse climatic backgrounds. It is noteworthy that moisture conditions have a substantial impact on the response of vegetation to air temperature on the TP. During the period of 1982–1998, under relatively insufficient moisture conditions, a positive correlation was observed between air temperature and surface vegetation in the humid and semi-humid regions of the southeastern TP, while a negative correlation was found in the semi-arid regions of northeastern TP. During 1998–2018, as moisture conditions became relatively sufficient, surface vegetation in the semi-arid regions showed positive correlations with both temperature and precipitation. However, surface vegetation in the humid and semi-humid regions exhibited a significant negative correlation with precipitation. During this period, the synergistic effects between warm and humid climates in the semi-arid regions of northeastern TP and warm and dry climates in humid and semi-humid regions of southeastern TP substantially enhanced surface vegetation on the TP. Furthermore, our results indicate that thermal factors (air temperature) primarily influence variations in surface vegetation within the high-altitude arid region of the TP. During 1998–2018, a significant cooling trend was observed in the northwestern TP, which could potentially account for the degradation of surface vegetation in the Kunlun Mountains. The findings of this study establish a scientific basis for the sustainable development of grassland ecosystems on the TP. Full article

This cross-cultural study seeks to (a) investigate the internal structure of the English version of the Satisfaction With Life Scale (SWLS) when administered to non-native English speakers (i.e., Asian individuals and (b) test for measurement invariance with its Italian counterpart). The whole sample comprises 338 participants, including 167 Asian international university students residing in the United States (50.3% females; M_age = 23.82, SD = 3.78) and 171 Italian university students living in Italy (69.6% females; M_age = 22.38, SD = 4.24). The dimensionality of the scale is assessed through confirmatory factor analysis (CFA), and multi-group confirmatory factor analysis (MG-CFA) is employed to examine measurement invariance. The results confirm the one-dimensionality of the SWLS [χ² = 9.815; df = 5; CFI = 0.989; TLI = 0.977; SRMR = 0.027]. Furthermore, achieving full strict invariance indicates that the SWLS items exhibit similar structures across both samples. The scale shows satisfactory internal reliability (α = 0.863, ω = 0.866). Overall, this study supports the cross-validity of the English version of the SWLS and underscores its robustness and suitability for assessing life satisfaction among non-native English speakers. Full article

Mechanical and thermal trauma during implant surgery may be reasons for initial peri-implant bone loss. Temperature development during drilling and implant insertion were quantified in this series of in vitro and animal experiments. Polyurethane foam material mimicking different classes of alveolar bone was used as a model material for simulating implant surgery. Using thermocouples, temperature development was determined in the model material at depths of 3 mm and 10 mm during site preparation and implant insertion. Additionally, an infrared camera allowed for measuring drill temperatures both in vitro and as part of an animal trial using an intraoral minipig model. Drill diameter and repeated usage of drills did not have a major effect on temperature generation. The addition of a diamond-like carbon coating, bone density, predrilling, and irrigation heavily affected intraosseous temperatures. In vivo, applying regular drill protocols, an intraosseous temperature rise of approximately 3 °K was determined. Implant geometry as well as the amount of undersizing of an osteotomy governed heat generation during implant insertion. Drill protocols and the amount of undersizing of an implant osteotomy constitute parameters by which clinicians can limit trauma during implant surgery. Full article

This study aimed to investigate the relationship between low-income elementary school students’ physical activity enjoyment and physical activity behavior and to determine if their motor competence predicts their physical activity enjoyment and physical activity behavior. A total of 82 students (51.2% male; M_age = 10, SD = 0.861) from two Title I elementary schools in the western United States were involved in this study. The students completed the Physical Activity Enjoyment Scale (PACES), the Physical Activity Questionnaire for Children (PAQ-C), and the Test of Gross Motor Development–Third Edition (TGMD-3). The results of the correlation analysis showed that physical activity enjoyment and physical activity behavior among the students were significantly related, r(80) = 0.379, p < 0.001. Multiple regression models indicated that locomotor skills were a significant predictor of the level of physical activity enjoyment (F_(2,79) = 8.334, p < 0.001; t = 3.76, p < 0.001), while motor competence did not predict the level of physical activity behavior. These findings illustrate the importance of both physical activity enjoyment and physical activity behavior and the significant role of locomotor skills in enhancing the level of physical activity enjoyment in this specific population of children. Motor competence should be considered a priority when designing and implementing physical education and physical activity programs for school-aged children from lower-income families. Full article

Modern production relies on data-based analytics for the prediction and optimization of production processes. Specialized data scientists perform tasks at companies and research institutions, dealing with real data from actual production environments. The roles of data preprocessing and data quality are crucial in data science, and an active research field deals with methodologies and technologies for this. While anecdotes and generalized surveys indicate preprocessing is the major operational task for data scientists, a detailed view of the subtasks and the domain of production data is missing. In this paper, we present a multi-stage survey on data science tasks in practice in the field of production. Using expert knowledge and insights, we found data preprocessing to be the major part of the tasks of data scientists. In detail, we found that tackling missing values, finding data point meanings, and synchronization of multiple time-series were often the most time-consuming preprocessing tasks. Full article

Bletilla striata is the dried tuber of B. striata (Thund.) Reichb.f., which has antibacterial, anti-inflammatory, anti-tumor, antioxidant and wound healing effects. Traditionally, it has been used for hemostasis therapy, as well as to treat sores, swelling and chapped skin. In this study, we used the ultraviolet (UV) absorbance rate of B. striata extracts as the index, and the extraction was varied with respect to the solid–liquid ratio, ethanol concentration, ultrasonic time and temperature in order to optimize the extraction process for its sunscreen components. The main compounds in the sunscreen ingredients of Baiji (B. striata) were analyzed using ultra-high-performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry. The sunscreen properties were subsequently evaluated in vitro using the 3M tape method. The results show that the optimal extraction conditions for the sunscreen components of B. striata were a solid–liquid ratio of 1:40 (g/mL), an ethanol concentration of 50%, an ultrasonic time of 50 min and a temperature of 60 °C. A power of 100 W and an ultrasonic frequency of 40 Hz were used throughout the experiments. Under these optimized conditions, the UV absorption rate of the isolated sunscreen components in the UVB region reached 84.38%, and the RSD was 0.11%. Eighteen compounds were identified, including eleven 2-isobutyl malic acid glucose oxybenzyl esters, four phenanthrenes, two bibenzyl and one α-isobutylmalic acid. An evaluation of the sunscreen properties showed that the average UVB absorption values for the sunscreen samples from different batches of B. striata ranged from 0.727 to 1.201. The sunscreen ingredients of the extracts from B. striata had a good UV absorption capacity in the UVB area, and they were effective in their sunscreen effects under medium-intensity sunlight. Therefore, this study will be an experimental reference for the extraction of sunscreen ingredients from the B. striata plant, and it provides evidence for the future development of B. striata as a candidate cosmetic raw material with UVB protection properties. Full article

Rigid porous polymeric monoliths are robust, highly efficient, versatile stationary phases. They offer simple preparation and convenient modification provided by a whole range of synthesis factors, e.g., starting monomers, cross-linkers, initiators, porogens, polymerization techniques, and temperature. The main aim of this study was to synthesize polymeric monoliths and determine the correlation between polymerization parameters and the porosity and thermal stability of the obtained materials. Polymeric monoliths were synthesized directly in HPLC columns using N-vinyl-2-pyrrolidone (NVP) and 4-vinylpiridine (4VP) as functional monomers, with trimethylolpropane trimethacrylate (TRIM) serving as the cross-linking monomer. During copolymerization a mixture of cyclohexanol/decane-1-ol was used as the pore-forming diluent. Polymerization was carried out at two different temperatures: 55 and 75 °C. As a result, monoliths with highly developed internal structure were synthesized. The value of their specific surface area was in the range of 92 m²/g to 598 m²/g, depending on the monomer composition and polymerization temperature. Thermal properties of the obtained materials were investigated by means of thermogravimetry (TG). Significant differences in thermal behavior were noticed between monoliths synthesized at 55 and 75 °C. Additionally, the poly(NVP-co-TRIM) monolith was successfully applied in GC analyses. Full article

In this work, we examine the possibility of converting a light propeller-driven aircraft, powered by a spark-ignition, reciprocating piston, and internal combustion engine running on AVGAS, into one powered by an electric motor driven by a proton exchange membrane fuel cell stack running on hydrogen. Our studies suggest that storing hydrogen cryogenically is a better option than storing hydrogen under pressure. In comparison to cryogenic tanks, high-pressure tanks are extremely heavy and unacceptable for light aircraft. We show that the modified aircraft (including batteries) is no heavier than the original, and that the layout of the major components results in lower movement of the aircraft center-of-gravity as the aircraft consumes hydrogen. However, we acknowledge that our fuel cell aircraft cannot store the same amount of energy as the original running on AVGAS. Therefore, despite the fact that the fuel cell stack is markedly more efficient than an internal combustion engine, there is a reduction in the range of the fuel cell aircraft. One of our most important findings is that the quantity of energy that we need to dissipate to the surroundings via heat transfer is significantly greater from a fuel cell stack than from an internal combustion engine. This is particularly the case when we attempt to run the fuel cell stack at high current densities. To control this problem, our strategy during the cruise phase is to run the fuel cell stack at its maximum efficiency, where the current density is low. We size the fuel cell stack to produce at least enough power for cruise, and when we require excess power, we add the energy stored in batteries to make up the difference. Full article

Arc (also known as Arg3.1) is an activity-dependent immediate early gene product enriched in neuronal dendrites. Arc plays essential roles in long-term potentiation, long-term depression, and synaptic scaling. Although its mechanisms of action in these forms of synaptic plasticity are not completely well established, the activities of Arc include the remodeling of the actin cytoskeleton, the facilitation of AMPA receptor (AMPAR) endocytosis, and the regulation of the transcription of AMPAR subunits. In addition, Arc has sequence and structural similarity to retroviral Gag proteins and self-associates into virus-like particles that encapsulate mRNA and perhaps other cargo for intercellular transport. Each of these activities is likely to be influenced by Arc’s reversible self-association into multiple oligomeric species. Here, we used mass photometry to show that Arc exists predominantly as monomers, dimers, and trimers at approximately 20 nM concentration in vitro. Fluorescence fluctuation spectroscopy revealed that Arc is almost exclusively present as low-order (monomer to tetramer) oligomers in the cytoplasm of living cells, over a 200 nM to 5 μM concentration range. We also confirmed that an α-helical segment in the N-terminal domain contains essential determinants of Arc’s self-association. Full article

The issue of bacterial infections in COVID-19 patients has received increasing attention. Scant data are available on the impact of bacterial superinfection and antibiotic administration on the outcome of hospitalized COVID-19 patients. We conducted a literature review from 1 January 2022 to 31 March 2024 to assess the current burden of bacterial infection and the evidence for antibiotic use in hospitalized COVID-19 patients. Published articles providing data on antibiotic use in COVID-19 patients were identified through computerized literature searches with the search terms [(antibiotic) AND (COVID-19)] or [(antibiotic treatment) AND (COVID-19)]. PubMed and SCOPUS databases were searched from 1 January 2022 to 31 March 2024. No attempt was made to obtain information about unpublished studies. English language restriction was applied. The quality of the included studies was evaluated by the tool recommended by the Joanna Briggs Institute. Both quantitative and qualitative information were summarized by means of textual descriptions. Five hundred fifty-one studies were identified, and twenty-nine studies were included in this systematic review. Of the 29 included studies, 18 studies were on the prevalence of bacterial infection and antibiotic use in hospitalized COVID-19 patients; 4 studies reported on the efficacy of early antibiotic use in COVID-19; 4 studies were on the use of sepsis biomarkers to improve antibiotic use; 3 studies were on the efficacy of antimicrobial stewardship programs and predictive models among COVID-19-hospitalized patients. The quality of included studies was high in 35% and medium in 62%. High rates of hospital-acquired infections were reported among COVID-19 patients, ranging between 7.5 and 37.7%. A high antibiotic resistance rate was reported among COVID-19 patients developing hospital-acquired infections, with a high in-hospital mortality rate. The studies evaluating multi-faceted antimicrobial stewardship interventions reported efficacy in decreasing antibiotic consumption and lower in-hospital mortality. Full article

Cochlear implantation is the therapy used for patients with severe to profound sensorineural hearing loss. For the success of the surgery, it is important that each surgical step is performed with meticulous precision, starting from the correct patient position on the operating table. In elderly or obese patients, this can be difficult to achieve due to cervical rigidity. With this technical note, we want to describe a new position from orthopedic surgery to perform a posterior tympanotomy accurately, ensuring a safe procedure and avoiding unpleasant complications. Full article

Recently, discriminative correlation filters (DCF) have been successfully applied for visual tracking. However, traditional DCF trackers tend to separately solve boundary effect and temporal degradation problems in the tracking process. In this paper, a variational online learning correlation filter (VOLCF) is proposed for visual tracking to improve the robustness and accuracy of the tracking process. Unlike previous methods, which use only first-order temporal constraints, this approach leads to overfitting and filter degradation. First, beyond the standard filter training requirement, our proposed VOLCF method introduces a model confidence term, which leverages the temporal information of adjacent frames during filter training. Second, to ensure the consistency of the temporal and spatial characteristics of the video sequence, the model introduces Kullback–Leibler (KL) divergence to obtain the second-order information of the filter. In contrast to traditional target tracking models that rely solely on first-order feature information, this approach facilitates the acquisition of a generalized connection between the previous and current filters. As a result, it incorporates joint-regulated filter updating. Through quantitative and qualitative analyses of the experiment, it proves that the VOLCF model has excellent tracking performance. Full article

The microphysical processes within the melting layer (ML) of stratiform clouds have been understudied, particularly regarding their intricate properties and behaviors. This study explores the ML’s microphysical characteristics in three distinct stratiform cloud occurrences over North China from 2017 to 2019. Our findings reveal that the reflectivity factor, coupled with the volume-weighted diameter (D_m), escalates within the upper and middle sections of the ML across all cases, suggesting that aggregation, primarily in the top 40% of the ML, significantly enhances the bright band phenomenon. Notably, the 2019 case (Spiral3) displayed more vigorous aggregation activities compared to the 2017 event (Spiral1), possibly due to larger initial particle sizes, leading to a swift increase in both mean and maximum particle diameters. Conversely, in the lower 60% of the ML, ongoing melting reduces mean particle diameters and potentially decreases total number concentration (N_t) due to accelerated particle descent. However, the 2018 case (Spiral2) deviated by showing a rapid N_t increase in the lowest 20% of the ML, where breakup mechanisms counteracted melting effects. The MLs in Spiral1 and Spiral3, in which aggregates were mainly formed by plate-like ice crystals, were thicker than those in Spiral2, dominated by low-density aggregates formed by the combination of needle and columnar ice crystals. This analysis underscores how variations in particle characteristics, such as habit, density, and size, along with thermodynamic conditions, dictate the onset temperature for melting, ML thickness, and dominant microphysical processes, which differ markedly among the cases. Full article

Inherited retinal diseases (IRDs) represent a frequent cause of blindness in children and adults. As a consequence of the phenotype and genotype heterogeneity of the disease, it is difficult to have a specific diagnosis without molecular testing. To date, over 340 genes and loci have been associated with IRDs. We present the molecular finding of 191 individuals with IRD, analyzed by targeted next-generation sequencing (NGS). For 67 of them, we performed a family segregation study, considering a total of 126 relatives. A total of 359 variants were identified, 44 of which were novel. Genetic diagnostic yield was 41%. However, after stratifying the patients according to their clinical suspicion, diagnostic yield was higher for well-characterized diseases such as Stargardt disease (STGD), at 65%, and for congenital stationary night blindness 2 (CSNB2), at 64%. Diagnostic yield was higher in the patient group where family segregation analysis was possible (68%) and it was higher in younger (55%) than in older patients (33%). The results of this analysis demonstrated that targeted NGS is an effective method for establishing a molecular genetic diagnosis of IRDs. Furthermore, this study underlines the importance of segregation studies to understand the role of genetic variants with unknow pathogenic role. Full article

The primary goal of pharmacology teaching is to prepare medical students to prescribe medications both safely and efficiently. At the Utrecht University Medical School, pharmacology is integrated into the three-year bachelor’s curriculum, primarily through large group sessions with limited interaction. A recent evaluation highlighted students’ appreciation for pharmacology teaching, but students admitted to attending these teaching moments unprepared, resulting in passive learning. To address this, team-based learning (TBL) was implemented to facilitate learning through interaction, critical thinking, problem solving and reflection through six steps, from superficial to deeper cognitive learning. This study, conducted over two academic years, assessed students’ perception and performance regarding TBL. Analysis of a digital questionnaire using a 5-point Likert scale showed high student satisfaction with TBL as a teaching methodology. However, confidence in pharmacology knowledge following TBL was moderate. TBL attendees outperformed non-attendees in pharmacology-related exam questions, indicating that TBL has a positive impact on student performance. We conclude that TBL is an engaging and effective method for pharmacology education, positively influencing student learning and performance. This method could be broadly applied for teaching pharmacology within the medical curriculum or other biomedical programs. Full article

The separation of a toluene/methanol/water ternary mixture is a difficult task due to the toluene/water and toluene/methanol azeotropes. In this article, low-energy pervaporation is proposed for the separation of the ternary azeotrope toluene–methanol–water. This work investigates the effects of feed temperature, feed flow rate, and vacuum on pervaporation and compares the energy consumption of pervaporation with that of distillation. The results showed that at the optimized flow rate of 50 L/h and a permeate side vacuum of 60 kPa at 50 °C, the water and methanol content in the permeate was about 63.2 wt.% and 36.8 wt.%, respectively, the water/ methanol separation factor was 24.04, the permeate flux was 510.7 g/m²·h, the water content in the feed out was reduced from 2.5 wt.% to less than 0.66 wt.%, and the dehydration of toluene methanol could be realized. Without taking into account the energy consumption of pumps and other power equipment, pervaporation requires an energy consumption of 43.53 kW·h to treat 1 ton of raw material, while the energy consumption of distillation to treat 1 ton of raw material is about 261.5 kW·h. Compared to the existing distillation process, the pervaporation process consumes much less energy (about one-sixth of the energy consumption of distillation). There is almost no effect on the surface morphology and chemical composition of the membrane before and after use. The method provides an effective reference for the dehydration of organic solvents from ternary mixtures containing toluene/methanol/water. Full article

Investigating aircraft flight dynamics often requires dynamic wind tunnel testing. This paper proposes a non-contact, off-board instrumentation method using vision-based techniques. The method utilises a sequential process of Harris corner detection, Kanade–Lucas–Tomasi tracking, and quaternions to identify the Euler angles from a pair of cameras, one with a side view and the other with a top view. The method validation involves simulating a 3D CAD model for rotational motion with a single degree-of-freedom. The numerical analysis quantifies the results, while the proposed approach is analysed analytically. This approach results in a 45.41% enhancement in accuracy over an earlier direction cosine matrix method. Specifically, the quaternion-based method achieves root mean square errors of 0.0101 rad/s, 0.0361 rad/s, and 0.0036 rad/s for the dynamic measurements of roll rate, pitch rate, and yaw rate, respectively. Notably, the method exhibits a 98.08% accuracy for the pitch rate. These results highlight the performance of quaternion-based attitude estimation in dynamic wind tunnel testing. Furthermore, an extended Kalman filter is applied to integrate the generated on-board instrumentation data (inertial measurement unit, potentiometer gimbal) and the results of the proposed vision-based method. The extended Kalman filter state estimation achieves root mean square errors of 0.0090 rad/s, 0.0262 rad/s, and 0.0034 rad/s for the dynamic measurements of roll rate, pitch rate, and yaw rate, respectively. This method exhibits an improved accuracy of 98.61% for the estimation of pitch rate, indicating its higher efficiency over the standalone implementation of the direction cosine method for dynamic wind tunnel testing. Full article

Background and Objective: Although extracorporeal membrane oxygenation (ECMO) is an essential life-saving technique for patients with refractory cardiopulmonary shock, it can be fatal in certain cases. Case Presentation: A 19-year-old girl treated with ECMO presented with acute limb ischemia 2 days after cannula removal. The decannulation was performed percutaneously by an interventional cardiologist, and the vascular surgery department was consulted after the patient developed symptoms. The first suspected diagnosis was thrombosis due to incorrect use of the closure device. However, the artery had ruptured due to the insertion of a catheter with a cannula that was larger than the patient’s artery. Management and Outcome: Fortunately, excessive bleeding due to the size-mismatched cannula was prevented by an unintentional complication of the closing device, which saved the patient’s life. She underwent a right common femoral artery thrombectomy and patch angioplasty. Hospital guidelines have changed regarding the surgical removal of ECMO cannulas. Discussion: This report aims to highlight the importance of two aspects that are critical to a successful outcome: individualized cannula selection followed by precise insertion and removal and postoperative evaluation of a patient’s final status. Full article