Background: With the development of some new antibody–drug conjugates, the HER2 classification of breast carcinomas now includes the HER2-low (H2L) category: IHC 1+, 2+ non-amplified by ISH, and double-equivocal carcinomas, mostly luminal, expressing hormone receptors (HR+). Methods: We analyzed mutational status and transcriptomic activities of three HER2 effector pathways: PI3K-AKT, MAPK, and JAK-STAT, in association with clinicopathologic features, in 62 H2L carcinomas compared to 43 HER2-positive and 20 HER2-negative carcinomas, all HR+. Results: H2L carcinomas had significantly lower histoprognostic grades and mitotic and Ki67 proliferation indexes than HER2-positive carcinomas. Their PIK3CA mutation rates were close to those of HER2-negative and significantly higher than in HER2-positive carcinomas, contrary to TP53 mutations. At the transcriptomic level, we identified three distinct groups which did not reflect the new HER2 classification. H2L and HER2-negative carcinomas shared most of clinicopathological and molecular characteristics, except HER2 membrane expression (mRNA levels). The presence of a mutation in a signaling pathway had a strong pathway activation effect. PIK3CA mutations were more prevalent in H2L carcinomas, leading to a strong activation of the PI3K-AKT signaling pathway even in the absence of HER2 overexpression/amplification. Conclusion: PIK3CA mutations may explain the failure of conventional anti-HER2 treatments, suggesting that new antibody–drug conjugates may be more effective. Full article

Background: This study aims to analyze the impact of the COVID-19 pandemic on the clinical, pathological, and surgical characteristics of acute appendicitis (AA) at the University Hospital Centre (UHC) Zagreb. Methods: This retrospective study analyzed demographic, clinical, and surgical data from consecutive AA patients. Data were collected from an electronic database for two periods: 1 January to 31 December 2019 (pre-COVID-19), and 11 March 2020, to 11 March 2021 (COVID-19 pandemic). Results: During the two study periods, 855 appendectomies were performed, 427 in the pre-pandemic, and 428 during the pandemic. Demographic data were comparable between groups. There was statistically no significant difference in the type of appendectomy (p = 0.33) and the median hospital length of stay (3; (2–5) days, p = 0.08). There was an increase in the conversion rate during the pandemic period (4.2% vs. 7.7%, p = 0.03). The negative appendectomy rate and the incidence of perforated AA did not differ significantly (p = 0.34 for both). Conclusions: We did not observe a significant increase in the rate of AA complications during the COVID-19 pandemic at the UHC Zagreb. This may be attributed to two factors: (1) AA was diagnosed and treated as an emergency, which remained available during the pandemic, and (2) diagnostic and therapeutic protocols remained unaltered. We recommend a laparoscopic approach even during the COVID-19 pandemic. Full article

Canine mammary tumors (CMTs) are the most common type of tumor in female dogs. In this study, we obtained a metastatic key protein, Fascin-1, by comparing the proteomics data of in situ tumor and metastatic cell lines from the same individual. However, the role of Fascin-1 in the CMT cell line is still unclear. Firstly, proteomics was used to analyze the differential expression of Fascin-1 between the CMT cell lines CHMm and CHMp. Then, the overexpression (CHMm-OE and CHMp-OE) and knockdown (CHMm-KD and CHMp-KD) cell lines were established by lentivirus transduction. Finally, the differentially expressed proteins (DEPs) in CHMm and CHMm-OE cells were identified through proteomics. The results showed that the CHMm cells isolated from CMT abdominal metastases exhibited minimal expression of Fascin-1. The migration, adhesion, and invasion ability of CHMm-OE and CHMp-OE cells increased, while the migration, adhesion, and invasion ability of CHMm-KD and CHMp-KD cells decreased. The overexpression of Fascin-1 can upregulate the Tetraspanin 4 (TSPAN4) protein in CHMm cells and increase the number of migrations. In conclusion, re-expressed Fascin-1 could promote cell EMT and increase lamellipodia formation, resulting in the enhancement of CHMm cell migration, adhesion, and invasion in vitro. This may be beneficial to improve female dogs’ prognosis of CMT. Full article

Rapidly changing conditions and the complexity and interconnectedness of global challenges means that learning across the lifespan is more important than ever. Equally critical are sustainable planetary futurities and associated pedagogical practices which reach beyond the imposition of settler temporalities, modernist technological solutions, and western cognitive imperialism as a means of responding to our cultural-ecological crisis. Pedagogical practices must actively work with diverse generational realities and impacts associated with the cultural, ecological, and climate emergency. This paper reports on a growing conversation across diverse cultural biospheres regarding inclusive Indigenous-led strategies of multi-generational resilience addressing human–environmental wellbeing. Adopting an inclusive Indigenist theoretical and methodological approach, it narrates the epistemological and relational practices of several multigenerational pedagogical forums (land-based and virtual) based in and out of Turtle Island/Canada from 2015–2023. Based on an Indigenist research paradigm and qualitative research methods pertaining to one multi-day land based learning summit and three online virtual learning forums, a thematic analysis of key findings relating to pedagogical practices, intercultural and intergenerational themes, and the shifting dynamics of multi-generational resilience work is provided. Themes include the critical importance of epistemological shifts over time; Indigenous multi spatial-temporalities; relational rather than binary or even hybrid views of sexual, gendered, ethnic, and racial identities within cultural-ecological restoration work; the relevance of transnational gatherings; and the adaption of pedagogical practices to meet fluctuating local–global conditions. The paper then summarizes the key elements of lifelong learning within an Indigenist approach to cultural-ecological restoration work, and concludes with a discussion regarding the relevance of this approach in reorientating three previously identified leverage points for accelerating sustainability transformation. Full article

The long-term use of copper (Cu)-based fungicide sprays in orchards is associated with changes in soil Cu levels. However, there is a gap in knowledge regarding the potential accumulation of Cu in orchards and the associated impacts on the soil microbial structure. This study assessed the possibility of Cu accumulation in different avocado orchard farms and further evaluated the potential effect on soil microbial activities. Soil Cu levels were quantified in Tauranga and Northland, and three avocado orchards were analysed in each experimental location. All avocado farms in both sites received Cu-based fungicide sprays for over eight years. Soil samples were collected at a 0–20 cm depth from all six orchards. The soil total and bioavailable Cu, changes in soil chemical properties, microbial biomass, dehydrogenase activity, alkaline phosphatase activity, and acid phosphatase activity were measured. The results revealed that the total Cu and bioavailable Cu concentrations in Tauranga orchards were 81.3 and 0.32, 196.7 and 0.82, and 33.6 and 0.31 mg Cu kg⁻¹ in Farms 1, 2, and 3, respectively. In Northland orchards, the total Cu and bioavailable Cu were 54.5 and 0.06, 18.4 and 0.77, and 46 and 0.34 mg Cu kg⁻¹ in Farm 1, 2, and 3, respectively. Five out of six of the avocado orchard farms assessed in this study had total Cu concentrations greater than 30 mg Cu kg⁻¹ reported in New Zealand native land. The magnitude of Cu accumulation was linked with soil pH and C content. No clear trend was observed between soil Cu concentrations and the soil microbial activity. Our study results demonstrated that the long-term use of Cu-based fungicide sprays can elevate Cu concentrations in orchard soils. Mitigation strategies need to be explored to abate the accumulation of Cu in orchard soils. Full article

Human melanoma is a highly aggressive malignant tumor originating from epidermal melanocytes, characterized by intrinsic resistance to apoptosis and the reprogramming of proliferation and survival pathways during progression, leading to high morbidity and mortality rates. This malignancy displays a marked propensity for metastasis and often exhibits poor responsiveness to conventional therapies. Fatty acids, such as n-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic and eicosapentaenoic acids, exert various physiological effects on melanoma, with increasing evidence highlighting the anti-tumorigenic, anti-inflammatory, and immunomodulatory properties. Additionally, n-3 PUFAs have demonstrated their ability to inhibit cancer metastatic dissemination. In the context of cancer treatment, n-3 PUFAs have been investigated in conjunction with chemotherapy as a potential strategy to mitigate severe chemotherapy-induced side effects, enhance treatment efficacy and improve safety profiles, while also enhancing the responsiveness of cancer cells to chemotherapy. Furthermore, dietary intake of n-3 PUFAs has been associated with numerous health benefits, including a decreased risk and improved prognosis in conditions such as heart disease, autoimmune disorders, depression and mood disorders, among others. However, the specific mechanisms underlying their anti-melanoma effects and outcomes remain controversial, particularly when comparing findings from in vivo or in vitro experimental studies to those from human trials. Thus, the objective of this review is to present data supporting the potential role of n-3 PUFA supplementation as a novel complementary approach in the treatment of malignant cancers such as melanoma. Full article

The continuous scanning laser Doppler vibrometry (CSLDV) technique is usually used to evaluate the vibration operational deflection shapes (ODSs) of structures with continuous surfaces. In this paper, an extended CSLDV is demonstrated to measure the non-continuous surface of the bladed disk and to obtain the ODS efficiently. For a bladed disk, the blades are uniformly distributed on a given disk. Although the ODS of each blade can be derived from its response data along the scanning path with CSLDV, the relative vibration direction between different blades cannot be determined from those data. Therefore, it is difficult to reconstruct the complete vibration mode of the whole blade disk. In order to measure the complete ODS of the bladed disk, a method based on ODS frequency response functions (ODS FRFs) has been proposed. While the ODS of each blade is measured by designing the suitable scanning paths in CSLDV, an additional response signal is obtained at a fixed point as the reference signal to identify the relative vibration phase between the blade and the blade of the bladed disk. Finally, a measurement is performed with a simple bladed disk and the results demonstrate the feasibility and effectiveness of the proposed extended CSLDV method. Full article

Antimicrobial peptides (AMPs) represent a promising antibiotic alternative to overcome drug-resistant bacteria by inserting into the membrane of bacteria, resulting in cell lysis. However, therapeutic applications of AMPs have been hindered by their ability to lyse eukaryotic cells. GF-17 is a truncated peptide of LL-37, which has perfect amphipathicity and a higher hydrophobicity, resulting in higher haemolytic activity. However, there is no significant difference in the cytotoxicity against human lung epithelial cells between the GF-17 and LL-37 groups, indicating that there are significant differences in the sensitivity of different human cells to GF-17. In this study, LL-37 and GF-17 were administered to mouse lungs via intranasal inoculation. Blood routine examination results showed that LL-37 did not affect the red blood cells, platelet, white blood cells and neutrophil counts, but GF-17 decreased the white blood cells and neutrophil counts with the increasing concentration of peptides. GF-17-treated mice suffer a body weight loss of about 2.3 g on average in 24 h, indicating that GF-17 is highly toxic to mice. The total cell counts in the bronchoalveolar lavage fluid from GF-17-treated mice were 4.66-fold that in the untreated group, suggesting that GF-17 treatment leads to inflammation in the lungs of mice. Similarly, the histological results showed the infiltration of neutrophils in the lungs of GF-17-treated mice. The results suggest that the administration of GF-17 in the lungs of mice does not affect the red blood cells and platelet counts in the blood but promotes neutrophil infiltration in the lungs, leading to an inflammatory response. Therefore, we established a mouse acute lung injury model to preliminarily evaluate the in vivo toxicity of AMPs. For AMPs with a clinical application value, systematic research is still needed to evaluate their acute and long-term toxicity. Full article

This paper presents investigations of rail vehicle bogies of the Y25 type. The Y25 bogie family is one of the most commonly used freight car bogie designs. In addition to several significant advantages characterising this design, several disadvantages have also been observed since the beginning of more than fifty years of its operation in several types of cargo vehicles. One of these defects observed in real systems is its “unsatisfactory running stability”, particularly for long straight tracks. This paper used the commercial engineering software VI-Rail (2010.13.0) to create a model of a gondola car (type 412W Eaos) with two Y25 bogies. The car model was tested in empty and loaded (maximum permissible load) modes. Its motion along straight and curved tracks with different radii values was analysed. The vehicle velocity was changed from a few m/s to the maximum values for which stable solutions of the model existed. For each route, the nonlinear critical velocity was determined, defining the maximum operating velocity of the modelled car. The model solutions were recorded, while just one was selected to present the results—the first wheelset’s lateral displacement y_lw. Conjecture about its “imperfect running quality” on curved tracks was confirmed. The possible appearance of self-exciting wheelset vibrations in the modelled car’s operating velocity range in a laden state was also observed. The research results on the impact of changes in the bogie suspension parameters on the vehicle model’s stability are presented. The crucial parameter in the bogie suspension was indicated. Reducing its value by several percent about the nominal value increases the critical velocity of the car to values higher than the maximum operating velocity of the modelled vehicle. Full article

Klinefelter syndrome (KS), also known as 47,XXY, is a genetic disorder characterized by the presence of an extra X chromosome. Despite the prevalence of verbal learning disabilities, memory impairments, and executive function deficits in individuals with KS, comprehensive research on the neuropsychological profiles of affected children and adolescents remains limited. Additionally, KS has been associated with comorbid conditions such as depression, anxiety, schizophrenia, attention-deficit hyperactivity disorder (ADHD), and autism spectrum disorders (ASDs). However, systematic investigations into the neuropsychological manifestations of KS in pediatric populations are scarce. Therefore, the primary objectives of this review are to provide an overview of key studies examining the neuropsychological profiles of children and adolescents with KS and to delineate the limitations and implications of existing research findings. By synthesizing available literature, this review aims to bridge the gap in understanding the cognitive and behavioral characteristics of children and adolescents with KS, shedding light on potential avenues for future research and clinical interventions. Ultimately, this review serves as a valuable resource for clinicians, researchers, policymakers, parents, and educators involved in the assessment and management of the neuropsychological aspects of Klinefelter syndrome in pediatric populations. Full article

Oxidative stress refers to the overproduction of reactive oxygen species and is often associated with numerous pathological conditions. Superoxide dismutase (SOD) is a widely used enzyme for evaluating oxidative stress, with numerous methods being developed for its detection in biological specimens like blood, urine, and saliva. In this study, a simple metal-assisted chemical etching method was employed for the fabrication of nanostructured silicon surfaces decorated with either silver dendrites or silver aggregates. Those surfaces were used as substrates for the immunochemical determination of SOD in synthetic saliva through surface-enhanced Raman spectroscopy (SERS) and surface-enhanced fluorescence (SEF). The immunoassay was based on a 3-step competitive assay format, which included, after the immunoreaction with the specific anti-SOD antibody, a reaction with a biotinylated secondary antibody and streptavidin. Streptavidin labeled with peroxidase was used in combination with a precipitating tetramethylbenzidine substrate for detection through SERS, whereas for SEF measurements, streptavidin labeled with the fluorescent dye Rhodamine Red-X was utilized. Both immunoassays were sensitive, with a detection limit of 0.01 μg/mL and a linear dynamic range from 0.03 to 3.3 μg/mL, enabling the evaluation of the oxidative stress status of an organism. Full article

The reasonable expression of live load and its accuracy are important to the safety and design rationality of highway bridge structures. In this study, the optimization issue of the traffic load model for the suspenders of large-scale suspension bridges is studied. Taking a 2300-m main span suspension bridge as an example, a method for suspender classification based on the geometric feature of the influence lines is proposed, and the extreme traffic load effect scenarios are analyzed and used as an optimization reference. Multi-objective optimization based on a genetic algorithm is used to explore the improvement of the traffic load model of the suspender. The traffic load model of the suspender is optimized with three objectives, i.e., accuracy, convenience, and improvement, and the optimization results regarding the load value and loading length are obtained. The value of the uniformly distributed load of the optimized model ranges from 6.4 kN/m to 8.9 kN/m, and the maximum value of the concentrated force could reach 1433 kN. By comparing the obtained optimized model with the current specification model and the extreme load effect scenario model, the improved applicability of the optimized model in the analysis of the load effect of the suspender can be verified. The optimized method and relevant conclusions can provide useful references for the improved design and operation management of similar bridge structures. Full article

Autonomous systems are becoming increasingly relevant in our everyday life. The transportation field is no exception and the smart cities concept raises new tasks and challenges for the development of autonomous systems development which has been progressively researched in literature. One of the main challenges is communication between different traffic objects. For instance, a mobile robot system can work as a standalone autonomous system reacting to a static environment and avoiding obstacles to reach a target. Nevertheless, more intensive communication and decision making is needed when additional dynamic objects and other autonomous systems are present in the same working environment. Traffic is a complicated environment consisting of vehicles, pedestrians, and various infrastructure elements. To apply autonomous systems in this kind of environment it is important to integrate object localization and to guarantee functional and trustworthy communication between each element. To achieve this, various sensors, communication standards, and equipment are integrated via the application of sensor fusion and AI machine learning methods. In this work review of vehicular communication systems is presented. The main focus is the researched sensors, communication standards, devices, machine learning methods, and vehicular-related data to find existing gaps for future vehicular communication system development. In the end, discussion and conclusions are presented. Full article

Centrifugal pumps are essential in many industrial processes. An accurate operation diagnosis of centrifugal pumps is crucial to ensure their reliable operation and extend their useful life. In real industry applications, many centrifugal pumps lack flowmeters and accurate pressure sensors, and therefore, it is not possible to determine whether the pump is operating near its best efficiency point (BEP). This paper investigates the detection of off-design operation and cavitation for centrifugal pumps with accelerometers and current sensors. To this end, a centrifugal pump was tested under off-design conditions and various levels of cavitation. A three-axis accelerometer and three Hall-effect current sensors were used to collect vibration and stator current signals simultaneously under each state. Both kinds of signals were evaluated for their effectiveness in operation diagnosis. Signal processing methods, including wavelet threshold function, variational mode decomposition (VMD), Park vector modulus transformation, and a marginal spectrum were introduced for feature extraction. Seven families of machine learning-based classification algorithms were evaluated for their performance when used for off-design and cavitation identification. The obtained results, using both types of signals, prove the effectiveness of both approaches and the advantages of combining them in achieving the most reliable operation diagnosis results for centrifugal pumps. Full article

Esterquats constitute a unique group of quaternary ammonium salts (QASs) that contain an ester bond in the structure of the cation. Despite the numerous advantages of this class of compounds, only two mini-reviews discuss the subject of esterquats: the first one (2007) briefly summarizes their types, synthesis, and structural elements required for a beneficial environmental profile and only briefly covers their applications whereas the second one only reviews the stability of selected betaine-type esterquats in aqueous solutions. The rationale for writing this review is to critically reevaluate the relevant literature and provide others with a “state-of-the-art” snapshot of choline-type esterquats and betaine-type esterquats. Hence, the first part of this survey thoroughly summarizes the most important scientific reports demonstrating effective synthesis routes leading to the formation of both types of esterquats. In the second section, the susceptibility of esterquats to hydrolysis is explained, and the influence of various factors, such as the pH, the degree of salinity, or the temperature of the solution, was subjected to thorough analysis that includes quantitative components. The next two sections refer to various aspects associated with the ecotoxicity of esterquats. Consequently, their biodegradation and toxic effects on microorganisms are extensively analyzed as crucial factors that can affect their commercialization. Then, the reported applications of esterquats are briefly discussed, including the functionalization of macromolecules, such as cotton fabric as well as their successful utilization on a commercial scale. The last section demonstrates the most essential conclusions and reported drawbacks that allow us to elucidate future recommendations regarding the development of these promising chemicals. Full article

Scenic parks in rural areas occupy crucial positions, serving as vital hubs for the daily lives of villagers and important leisure destinations for tourists. This research is dedicated to assessing the significance of the scenic park in Wawuzhuang Village from the perspectives of both villagers and tourists. Employing a comprehensive research approach, this study examines the current state of the scenic park and its ability to meet the requirements of villagers and tourists. Villagers, mainly elderly individuals with low levels of education, rely heavily on green spaces for leisure and social interaction. In contrast, younger tourists and villagers require spaces that offer more comfort and aesthetic appeal. This study highlights the possibility of designing rural scenic parks to meet the basic needs of both tourists and villagers. In addition, mixed-use designs and public participation designs are suggested to promote the use of rural parks, potentially creating an attractive community environment that attracts new villagers and more tourists. Although this study is limited to a single case, it lays the groundwork for further research in other rural areas. This research contributes to the sustainable development of rural revitalization. Full article

In order to further reduce the height of burrs on the surface of the workpiece when milling titanium alloy with ball-end milling cutters and optimize the quality of the workpiece, this article takes the mesoscopic geometric feature of ball-end milling cutters as the research object and establishes the theoretical relationship between the mesoscopic geometric feature parameters and the height of titanium alloy burrs during milling. A milling test platform was built to explore the influence of micro-texture, blunt edge single factor, and their interaction with cutting parameters on the force-thermal characteristics and workpiece burr in the milling process. The influence mechanism was revealed, and the prediction model was established. The results show that the ball-end milling cutter with mesoscopic geometric features was able to suppress burrs, and the burr height was reduced by 21% compared with the non-textured milling cutter. The micro-texture reduced the contact area and improved the heat dissipation conditions, thereby reducing the force-thermal characteristics and thus inhibiting the formation of some burrs. The blunt edge can disperse the stress, diffuse the local heat in the tool–chip contact area, and reduce the burr height. In the interaction test considering cutting parameters, the interaction between R and ap was significant. The optimized parameters based on the simulated annealing algorithm were as follows: the radius of the blunt edge was 33.242 μm, the distance between the texture and the edge was 114.621 μm, the texture diameter was 59.820 μm, the texture spacing L₁ was 131.410 μm, the cutting depth a_p was 0.310 mm, the cutting speed V was 140.019 mm/min, and the feed f was 60 μm/z. This provides a basis for the study of strengthening the tool to suppress burr size. Full article

As a hydrocarbon-rich depression within the Bohai Bay Basin, the Huanghekou Depression is a focal region for exploring hydrocarbons in the eastern China Sea. Previous studies have insufficiently examined the correlation between the enrichment of organic matter and the environments in which it is deposited. Herein, the hydrocarbon potential, palaeoclimate, sedimentary environment, organic matter sources, and organic matter enrichment of the source rocks of the Shahejie Formation in the Huanghekou Depression were investigated using organic and inorganic geochemical indicators. The organic matter type of the source rock in Huanghekou Depression’s Shahejie Formation was predominantly Type II, with a minor presence of Type III. Furthermore, the source rock had a poor-to-good comprehensive evaluation grade in E₃s_1–2, whereas E₂s₃ and E₂s₄ had medium-to-good comprehensive evaluations in their source rocks. In terms of maturity, E₃s₁ was in an intermediate position between the immature and mature stages and E₃s₂ and E₂s₃ were between the low-maturity and mature stages, whereas E₂s₄ transitioned into full maturity. Biomarkers and sensitive element indicators indicated that the organic matter in E₃s_1–2 was primarily derived from lower aquatic organisms and algae. This palaeoclimate was characterised by aridity, a water body containing saline and semi-saline water, and a strongly reducing environment resulting from water body stratification, leading to oxygen deficiency. The organic matter in E₂s₃ was primarily derived from aquatic organisms and algal inputs; these deposits were formed in a reduced environment characterised by relatively low salinity, ranging between semi-saline and freshwater conditions. The organic matter enrichment model of the Shahejie Formation was established based on sedimentary environment, palaeoclimatic, and organic matter source analyses, utilising E₃s_1–2 as preservation models and E₂s₃ as the productivity model. This study provides a basis for in-depth exploration and advancement of oil and gas reserves. Full article

The process of transformation of the silicate components of the crystal lattice structure of chrysotile during its quantitative interaction with aqueous solutions containing various stoichiometrically required amounts of sulfuric acid (SRA H₂SO₄) calculated with respect to the magnesium content in the composition of chrysotile is investigated. It has been shown by IR spectroscopic, X-ray phase, thermal and chemical methods of investigation and analysis that, with quantitative interactions of chrysotile and sulfuric acid, first of all, the “brucite layer” of the molecular structural structure of chrysotile is exposed to acid at SRA H₂SO₄ = 0.1–0.3. As a result of ion exchange processes, acidic silanol (≡Si–O–H) or disilanol (=Si=(O–H)₂) bonds are formed. These acid groups form one-dimensional silicate chains with transverse bridges (≡Si–O–Si≡), where the angles (Si–O–Si = 180°C) straighten, which are recorded in the IR spectra in the region of characteristic absorption of 1220–1250 cm⁻¹ silica. The association of the resulting acid groups into silicate chains, dimers, and trimers with transverse bridges, leads to the appearance of colloidal silica particles in the system, which cause some inhibition of the dissolution of layered magnesium hydrosilicate in sulfuric acid solutions containing H₂SO₄˃ 0.3 SRA. Full article

Introduction: Contraceptive illiteracy leads to non-adherence, discontinuation, and dissatisfaction with the method. Person-centered contraceptive counseling is based on quality care on a communicative basis that promotes shared decision-making, leading to a choice adapted to the woman’s needs, lifestyle, and health condition. We intend to build and validate an instrument that serves as a guide for quality contraceptive counseling, facilitating decision-making about contraceptive methods. Methods: We used the Delphi method in a total of two rounds. The content was validated through a panel of eighteen experts with experience in teaching, research in contraceptive counseling, and obstetric nursing. To assess the consensus and stability of the responses, two questionnaires were administered and the Content Validity Index and Content Validity Ratio were calculated. Results: The initial version of the guide, consisting of six indicators and thirty-five items, was submitted to the panel of experts to obtain consensus and stability from respondents (first round). The results showed a response rate of 66.0%; thirty-four indicators reached consensus and one did not reach consensus. Suggestions for modifying the indicator were received by the experts and incorporated in the next round. In the second round, the response rate increased to 78.0%. Two indicators were resubmitted, of which one was accepted. This resulted in the final version of the instrument, with six points and thirty-five items. Discussion: The guide proved to be a valid tool for nurse–midwives to provide quality contraceptive advice to women, allowing them to make autonomous and informed choices regarding their sexual and reproductive health. Full article

Slimy sculpin (Uranidea cognata) inhabit coldwater streams in southeastern Minnesota, USA, many of which were subjected to probable 2000-year flood events in August 2007. Floods scoured streambeds, created new stream channels, and greatly reduced benthic invertebrate communities that serve as the primary food resource for sculpin. Diets and Fulton condition of sculpin in Gilmore Creek (with moderate flooding) and Garvin Brook (with very severe flooding) had been examined just prior to flooding and were re-examined 2 weeks after flooding to assess possible diet and condition changes. Diets, body condition, and reproductive fitness of sculpin were examined 7 months post-flood in these same two streams, plus nearby Trout Run (which also experienced very severe flooding). Sculpin condition declined slightly post-flood in Garvin Brook but improved in Gilmore Creek. Prior to spring spawning, the condition of Garvin Brook sculpin had improved, but Gilmore Creek fish condition had worsened. Sculpin diets were more diverse before and after flooding in Gilmore Creek than in Garvin Brook, although the diets of fish from both streams were dominated (>55%) by midge (Diptera: Chironomidae) larvae. Diets remained largely unchanged before versus after flooding in the more severely flooded Garvin Brook, but they changed in Gilmore Creek, becoming more midge-dominated. Prey number per sculpin stomach declined post-flood in Gilmore Creek but not in Garvin Brook, although the dry mass of prey/fish wet mass declined post-flood in both streams. Pre-spawn sculpin displayed no patterns in reproductive fitness (gonadosomatic index, hepatosomatic index, oocyte number) among the three streams that may have been related to flooding severity the previous summer. Sculpin diets and condition were not altered as expected by flooding, and food resource recovery apparently was rapid enough to prevent longer-term impacts on sculpin condition and reproductive fitness in the streams examined. Full article

Organizations are increasingly depending on their employees to contribute suggestions aimed at enhancing organizational processes, boosting overall efficiency, and fostering innovation. However, some factors might hinder employees from expressing their thoughts. While there is evidence suggesting an effect of supervisor social undermining behavior on employee voice, the impact on innovative types of voice, specifically promotive voice, remains uncertain. This study aims to explore the association between supervisor social undermining and employee promotive voice. Moreover, this research investigates how employee perceptions of procedural justice moderate this relationship, utilizing the Conservation of Resources theory. Data were collected from 115 highly skilled employees, and hierarchical regression analysis was employed to assess the proposed hypotheses. The findings suggest that when individuals encounter social undermining behavior from their supervisor, they are less inclined to engage in promotive voice behavior. Interestingly, the results indicate that this relationship becomes stronger when individuals possess higher perceptions of procedural justice. To the best of our knowledge, this study is the first to investigate the impact of supervisor social undermining on promotive voice while considering procedural justice as a moderator in this relationship. The findings of this study suggest several theoretical and practical implications and provide directions for future research. Full article