Osteoclast stimulatory transmembrane protein (OC-STAMP) plays a pivotal role in the promotion of cell fusion during osteoclast differentiation (osteoclastogenesis) in the context of pathogenic bone resorption. Thus, it is plausible that the suppression of OC-STAMP through a bioengineering approach could lead to the development of an effective treatment for inflammatory bone resorptive diseases with minimum side effects. Here, we synthesized two types of spermine-bearing (Spe) cationic glucan dendrimer (GD) gels (with or without C12) as carriers of short interfering RNA (siRNA) to silence OC-STAMP. The results showed that amphiphilic C12-GD-Spe gel was more efficient in silencing OC-STAMP than GD-Spe gel and that the mixture of anti-OC-STAMP siRNA/C12-GD-Spe significantly downregulated RANKL-induced osteoclastogenesis. Also, local injection of anti-OC-STAMP-siRNA/C12-GD-Spe could attenuate bone resorption induced in a mouse model of periodontitis. These results suggest that OC-STAMP is a promising target for the development of a novel bone regenerative therapy and that C12-GD-Spe gel provides a new nanocarrier platform of gene therapies for osteolytic disease. Full article

Mycoplasma capricolum subsp. capricolum (Mcc) and Mycoplasma capricolum subsp. capripneumoniae (Mccp) are pathogens that affect large and small ruminants. Indeed, Mcc affects both sheep and goats, causing contagious agalactia (CA). Mccp affects only goats, causing contagious caprine pleuropneumonia (CCPP). CA and CCPP are mainly controlled using inactivated Mcc and Mccp vaccines. However, producing the vaccine with the Mccp strain is complex, fastidious, and costly due to the slow growth of the bacterium. In this study, we present new oil-adjuvanted and inactivated Mcc and Mccp vaccines for sheep and goats against CA and CCPP. The vaccines were evaluated for safety and efficacy using experimental infection. A serological response was observed one week after of the first vaccination of sheep and goats with Mcc and goats with Mccp. The vaccinated animals were subsequently challenged with the virulent Mcc MOR20 strain. The Mcc vaccine was demonstrated to provide robust protection when the animals were challenged with Mcc MOR20. Cross-protection against the Mcc MOR20 challenge was also obtained with the Mccp vaccine. This finding revealed, for the first time, the safety and efficacy of an inactivated Mcc vaccine against contagious agalactia and cross-protection between Mcc and Mccp strains. Full article

Pore water pressure (PWP) response is significant for evaluating the earth dams’ stability, and PWPs are, therefore, generally monitored. However, due to the soil heterogeneity and its non-linear behavior within earths, the PWP is usually difficult to estimate and predict accurately in order to detect a pathology or anomaly in the behavior of an embankment dam. This study endeavors to tackle this challenge through the application of diverse machine learning (ML) techniques in estimating the PWP within an existing earth dam. The methods employed include random forest (RF) combined with simulated annealing (SA), multilayer perceptron (MLP), standard recurrent neural networks (RNNs), and gated recurrent unit (GRU). The prediction capability of these techniques was gauged using metrics such as the coefficient of determination (R²), mean square error (MSE), and CPU training time. It was found that all the considered ML methods could give satisfactory results for the PWP estimation. Upon comparing these methods within the case study, the findings suggest that, in this study, multilayer perceptron (MLP) gives the most accurate PWP prediction, achieving the highest coefficient of determination (R² = 0.99) and the lowest mean square error (MSE = 0.0087) metrics. A sensitivity analysis is then presented to evaluate the models’ robustness and the hyperparameter’s influence on the performance of the prediction model. Full article

Recently, an outbreak of Ips sexdentatus (Börner, 1776) has caused considerable damage in the pine forests of the Czech Republic. As historical data on the biology of this pest are scarce due to its rare occurrence in recent decades, our work focused on monitoring flight activity and voltinism and investigating methods for monitoring its activity during the growing season. Observations were conducted from March to September 2021 and 2022 at three sites using 12 Theysohn traps with four types of pheromone lures (ACUMIPROTECT, ACUWIT, SEXOWIT and Pheagr IAC) together with data loggers to record weather conditions. The first beetles occurred in early May (daily mean temperatures above 13 °C). After the first egg laying stage, females re-emerged to establish a sister brood. The maximum flight activity appeared between late June and mid-July (daily mean temperatures about 20 °C), and the offspring occurred at the turn of June/July and in the first half of August. Since then, flight activity had a downward trend and quietened in September. According to the data, monitoring of I. sexdentatus should be conducted between May and September using the ACUMIPROTECT pheromone bait exhibiting the highest capturing efficacy. In future, however, the behavior of I. sexdentatus might alter due to climate change. Full article

As the Internet of Things (IoT) continues to evolve, securing IoT networks and devices remains a continuing challenge. Anomaly detection is a crucial procedure in protecting the IoT. A promising way to perform anomaly detection in the IoT is through the use of machine learning (ML) algorithms. There is a lack of studies in the literature identifying optimal (with regard to both effectiveness and efficiency) anomaly detection models for the IoT. To fill the gap, this work thoroughly investigated the effectiveness and efficiency of IoT anomaly detection enabled by several representative machine learning models, namely Extreme Gradient Boosting (XGBoost), Support Vector Machines (SVMs), and Deep Convolutional Neural Networks (DCNNs). Identifying optimal anomaly detection models for IoT anomaly detection is challenging due to diverse IoT applications and dynamic IoT networking environments. It is of vital importance to evaluate ML-powered anomaly detection models using multiple datasets collected from different environments. We utilized three reputable datasets to benchmark the aforementioned machine learning methods, namely, IoT-23, NSL-KDD, and TON_IoT. Our results show that XGBoost outperformed both the SVM and DCNN, achieving accuracies of up to 99.98%. Moreover, XGBoost proved to be the most computationally efficient method; the model performed 717.75 times faster than the SVM and significantly faster than the DCNN in terms of training times. The research results have been further confirmed by using our real-world IoT data collected from an IoT testbed consisting of physical devices that we recently built. Full article

Schnitzler syndrome is a rare disorder characterized by a chronic urticarial rash associated with immunoglobulin M (IgM) monoclonal gammopathy. Schnitzler syndrome shares strong clinicopathologic similarities with monogenic IL-1-mediated autoinflammatory disorders and is now considered an acquired adult-onset autoinflammatory disease. The spectacular effect of interleukin-1 inhibitors demonstrates the key role of this cytokine in the pathogenesis of the disease. However, the physiopathology of Schnitzler syndrome remains elusive, and the main question regarding the relationship between autoinflammatory features and monoclonal gammopathy is still unanswered. The purpose of this narrative review is to describe what is currently known about the pathogenesis of this peculiar disease, as well as to address its diagnosis and management. Full article

Clear-cell Renal-Cell Carcinoma (ccRCC) is the most common type of renal-cell carcinoma (RCC). In many cases, RCC patients manifest the first symptoms during the advanced stage of the disease. For this reason, immunotherapy appears to be one of the dominant treatments to achieve a resolution. In this review, we focus on the presentation of the main immune checkpoint proteins that act as negative regulators of immune responses, such as PD-1, CTLA-4, LAG-3, TIGIT, and TIM-3, and their respective inhibitors. Interleukin-2, another potential component of the treatment of ccRCC patients, has also been covered. The synergy between several immunotherapies is one of the main aspects that unites the conclusions of research in recent years. To date, the combination of several immunotherapies enhances the efficacy of a monotherapy, which often manifests important limitations. Immunotherapy aimed at restoring the anti-cancer immune response in ccRCC, involved in the recognition and elimination of cancer cells, may also be a valid solution for many other types of immunogenic tumors that are diagnosed in the final stages. Full article

Endothelial cells (ECs) line the inner surface of all blood vessels and form a barrier that facilitates the controlled transfer of nutrients and oxygen from the circulatory system to surrounding tissues. Exposed to both laminar and turbulent blood flow, ECs are continuously subject to differential mechanical stimulation. It has been well established that the shear stress associated with laminar flow (LF) is atheroprotective, while shear stress in areas with turbulent flow (TF) correlates with EC dysfunction. Moreover, ECs show metabolic adaptions to physiological changes, such as metabolic shifts from quiescence to a proliferative state during angiogenesis. The AMP-activated protein kinase (AMPK) is at the center of these phenomena. AMPK has a central role as a metabolic sensor in several cell types. Moreover, in ECs, AMPK is mechanosensitive, linking mechanosensation with metabolic adaptions. Finally, recent studies indicate that AMPK dysregulation is at the center of cardiovascular disease (CVD) and that pharmacological targeting of AMPK is a promising and novel strategy to treat CVDs such as atherosclerosis or ischemic injury. In this review, we summarize the current knowledge relevant to this topic, with a focus on shear stress-induced AMPK modulation and its consequences for vascular health and disease. Full article

Phenotypic variation analysis and comprehensive evaluation are important tools for selecting provenances of forest trees, which, in turn, is crucial for genetic improvement of forest trees. The study analyzed eight needle-leaf and fourteen tracheid trait indexes of ten provenances of Qinghai spruce forest stands. Multiple comparisons, correlation analysis, cluster analysis, and principal component analysis were used to evaluate the differences in needle-leaf and tracheid traits among the different provenances. The study found significant differences in the needle-leaf and tracheid traits among the provenances. All traits included 50 pairs of positive and 26 pairs of negative correlations. The coefficient of variation for the tracheid trait (18.86%) was higher than that for the needle-leaf trait (17.20%). A comprehensive evaluation of needle-leaf and tracheid traits was conducted using principal component analysis. The study demonstrated that the degree of variation in needle-leaf and tracheid traits among the provenances of Qinghai spruce was extensive, with a multitude of sources of variation. Therefore, it is crucial to strengthen the selection of good provenances in the early seed selection process. The results of the comprehensive evaluation can be utilized as a foundation for the selection of good provenances for carbon sequestration and timber properties. Full article

South Korea is a rapidly aging society with the lowest fertility rates among the OECD economies. It is projected to become a super-aged society in 2025, with the share of individuals older than 65 reaching twenty percent. These developments make it important to analyze the determinants of health outcomes in older individuals. In this study, we identified the determinants of subjective and objective health outcomes among senior individuals in South Korea. We used self-rated health and life satisfaction scores as the two subjective health status indicators, while the number of chronic diseases was the objective one. We ran Tobit multivariate regressions of all three indicators on a set of factors related to the older citizens’ physical, economic, and social characteristics. Active employment status and willingness to work in the future were positively related to self-rated health level but were not statistically related to life satisfaction, while income positively affected both subjective health status indicators. Age did not appear to affect satisfaction with life. Active leisure activities were positively related to both self-rated health and life satisfaction. In contrast, passive leisure, such as watching TV, was negatively related to both health status indicators while being associated with an increased number of chronic diseases. Our findings suggest that older South Koreans view employment primarily as a means of financial support rather than as an opportunity for active social engagement. Full article

Acemetacin (ACM) is a new non-steroidal anti-inflammatory drug with anti-inflammatory, analgesic, and antipyretic effects. However, the poor water solubility and gastrointestinal side effects limit its use. Recently, the co-amorphous (CAM) strategy has attracted great interest to improve solubility for poorly water-soluble drugs, and basic amino acids have the potential to protect the gastrointestinal tract. In order to develop a highly efficient and low-toxic ACM formulation, we prepared ACM CAM systems, with basic amino acids (lysine, arginine, and histidine) as co-formers, using a cryo-milling method. The solid-state behaviors of the ACM CAM systems were characterized by polarizing light microscopy, differential scanning calorimetry, and powder X-ray diffraction. Fourier transform infrared spectroscopy and molecular docking were carried out to understand the formation mechanism. Moreover, the gastro-protective effects of ACM CAM systems were evaluated in a rat gastric ulcer model. The results demonstrated that the CAM systems improved the dissolution rates of ACM compared with the neat amorphous counterpart. Furthermore, ACM CAM systems are significantly effective in mitigating the ACM-induced gastric ulcer in rats, and the ulcer inhibition rates were almost 90%. More importantly, this study provided a useful method for mitigating drug-induced gastrointestinal damage and broadened the applications of drug–amino acid CAM systems. Full article

We have been encouraging practicing gynecologists to adopt molecular diagnostics tests, PCR, and cancer biomarkers, as alternatives enabled by these platforms, to traditional Papanicolaou and colposcopy tests, respectively. An aliquot of liquid-based cytology was used for the molecular test [high-risk HPV types, (HR HPV)], another for the PAP test, and one more for p16/Ki67 dual-stain cytology. A total of 4499 laboratory samples were evaluated, and we found that 25.1% of low-grade samples and 47.9% of high-grade samples after PAP testing had a negative HR HPV-PCR result. In those cases, reported as Pap-negative, 22.1% had a positive HR HPV-PCR result. Dual staining with p16/Ki67 biomarkers in samples was positive for HR HPV, and 31.7% were also positive for these markers. Out of the PCR results that were positive for any of these HR HPV subtypes, n 68.3%, we did not find evidence for the presence of cancerous cells, highlighting the importance of performing dual staining with p16/Ki67 after PCR to avoid unnecessary colposcopies. The encountered challenges are a deep-rooted social reluctance in Mexico to abandon traditional Pap smears and the opinion of many specialists. Therefore, we still believe that colposcopy continues to be a preferred procedure over the dual-staining protocol. Full article

Concrete infrastructure is vulnerable to damage such as spalling. Spalling leads to chloride penetration, which causes internal corrosion, weakens structural stability and durability, and increases the likelihood of additional cracks or damage, consequently necessitating repair. Existing repair methods do not consider the factors that affect damage in the repaired areas, so additional damage can occur, making monitoring necessary to identify these factors. Nevertheless, existing studies have not adequately addressed the monitoring of internal conditions within concrete, making it difficult to manage such damage effectively. Accordingly, in this study, a monitoring system was developed using building information modeling (BIM) and the Internet of Things (IoT) to better identify internal changes in concrete pavements. Employing embedded sensors to measure temperature, humidity, and stress within the concrete, our system uses Dynamo scripts for real-time data visualization within BIM. Validated against the ASTM D8292 standard, this system captures and analyzes environmental impacts on concrete. This integration facilitates the detection of internal changes, allowing for the real-time visualization of these impacts. This study can help establish repair plans by identifying factors affecting concrete, contributing to preventive maintenance, potentially reducing maintenance costs, and enhancing the sustainability of concrete infrastructures. Full article

The demand for sustainable and cost-effective materials for photovoltaic technology has led to an increasing interest in Cu₃BiS₃ thin films as potential absorber layers. This review provides a comprehensive overview of the main physical properties, synthesis methods, and theoretical studies of Cu₃BiS₃ thin films for photovoltaic applications. The high optical absorption coefficient and band gap energy around the optimal 1.4 eV make Cu₃BiS₃ orthorhombic Wittichenite-phase a promising viable alternative to conventional thin film absorber materials such as CIGS, CZTS, and CdTe. Several synthesis techniques, including sputtering, thermal evaporation, spin coating, chemical bath deposition, and spray deposition, are discussed, highlighting their impact on film quality and photovoltaic performance. Density Functional Theory studies offer insights into the electronic structure and optical properties of Cu₃BiS₃, aiding in the understanding of its potential for photovoltaic applications. Additionally, theoretical modeling of Cu3BiS3-based photovoltaic cells suggests promising efficiencies, although experimental challenges remain to be addressed. Overall, this review underscores the potential of CBS thin films as sustainable and cost-effective materials for future PV technology while also outlining the ongoing research efforts and remaining challenges in this field. Full article

Firm competitiveness is a crucial trait of companies which managers strive to improve, and even policy makers focus on it regarding economic development. As companies are embedded into different layers of the environment, this study aims to identify the influence of top managerial perception about the external environment on the firm-level competitiveness of medium-sized and large internationalized companies. The investigation is based on the 6th round of the Competitiveness Survey conducted in Hungary between October 2018 and July 2019 from which n = 107 medium-sized or large companies qualified for this study. The institution-based view from the strategic management literature was chosen to differentiate the variables in the macroeconomic environment of the firm while the FCI index was used to measure firm-level competitiveness. After conducting factor and regression analyses, the results show that the perceived macro environment is positively related to the competitiveness of internationalized medium-sized and large companies. Managerial perceptions on civil service and education had the strongest significant positive association with firm-level competitiveness, whereas perceptions on social and ecological expectations had a significant positive association with the Adaptivity of the firm. This study contributes to the existing body of literature by demonstrating that, even within contentious institutional contexts, the perceptions of internationalized medium and large companies’ top managers regarding education, civil service, and social and ecological expectations have a discernible influence on the competitiveness of these companies. Full article

The incidence of early onset colorectal cancer (EOCRC) in Canada has increased. To address the growing incidence of EOCRC, Colorectal Cancer Canada (CCC) developed the Never Too Young (N2Y) program to identify gaps in care and evaluate patient and caregiver experiences with CRC. The survey was available online using SurveyMonkey across Canada between 12 December 2022 and 1 May 2023. The patient and caregiver survey consisted of 113 and 94 questions, respectively. A total of 108 EOCRC patients and 20 caregivers completed the survey. Many respondents were unaware of EOCRC (41.6%) and the disease symptoms (45.2%) before diagnosis. Patient age at diagnosis was between 45 and 50 years in 31.7%, and 72.8% of them were diagnosed at stage III or IV. A perception of an initial misdiagnosis was common (67.4%) for EOCRC patients, and 51.2% felt dismissed due to their age. Patients and caregivers reported impacts of EOCRC on their mental health, with 70.9% of patients expressing a need for support with depression and 93.3% of caregivers experiencing a constant fear of recurrence of their loved one’s cancer. Improving the Canadian population’s awareness of EOCRC (e.g., CRC symptoms) is important for ensuring timely diagnoses. Similarly, it is critical to ensure that healthcare providers are aware of the increase in EOCRC cases and the unique needs of these patients. Re-evaluation of the CRC screening age should be undertaken in Canada to determine whether lowering the start age to 45 years will improve outcomes in this demographic. Full article

Clostridium perfringens is the main pathogen of chicken necrotic enteritis (NE) causing huge economic losses in the poultry industry. Although dietary secondary bile acid deoxycholic acid (DCA) reduced chicken NE, the accumulation of conjugated tauro-DCA (TDCA) raised concerns regarding DCA efficacy. In this study, we aimed to deconjugate TDCA by bile salt hydrolase (BSH) to increase DCA efficacy against the NE pathogen C. perfringens. Assays were conducted to evaluate the inhibition of C. perfringens growth, hydrogen sulfide (H₂S) production, and virulence gene expression by TDCA and DCA. BSH activity and sequence alignment were conducted to select the bsh gene for cloning. The bsh gene from Bifidobacterium longum was PCR-amplified and cloned into plasmids pET-28a (pET-BSH) and pDR111 (pDR-BSH) for expressing the BSH protein in E. coli BL21 and Bacillus subtilis 168 (B-sub-BSH), respectively. His-tag-purified BSH from BL21 cells was evaluated by SDS-PAGE, Coomassie blue staining, and a Western blot (WB) assays. Secretory BSH from B. subtilis was analyzed by a Dot-Blot. B-sub-BSH was evaluated for the inhibition of C. perfringens growth. C. perfringens growth reached 7.8 log10 CFU/mL after 24 h culture. C. perfringens growth was at 8 vs. 7.4, 7.8 vs. 2.6 and 6 vs. 0 log10 CFU/mL in 0.2, 0.5, and 1 mM TDCA vs. DCA, respectively. Compared to TDCA, DCA reduced C. perfringens H₂S production and the virulence gene expression of asrA1, netB, colA, and virT. BSH activity was observed in Lactobacillus johnsonii and B. longum under anaerobe but not L. johnsonii under 10% CO₂ air. After the sequence alignment of bsh from ten bacteria, bsh from B. longum was selected, cloned into pET-BSH, and sequenced at 951 bp. After pET-BSH was transformed in BL21, BSH expression was assessed around 35 kDa using Coomassie staining and verified for His-tag using WB. After the subcloned bsh and amylase signal peptide sequence was inserted into pDR-BSH, B. subtilis was transformed and named B-sub-BSH. The transformation was evaluated using PCR with B. subtilis around 3 kb and B-sub-BSH around 5 kb. Secretory BSH expressed from B-sub-BSH was determined for His-tag using Dot-Blot. Importantly, C. perfringens growth was reduced greater than 59% log10 CFU/mL in the B-sub-BSH media precultured with 1 vs. 0 mM TDCA. In conclusion, TDCA was less potent than DCA against C. perfringens virulence, and recombinant secretory BSH from B-sub-BSH reduced C. perfringens growth, suggesting a new potential intervention against the pathogen-induced chicken NE. Full article

Knowledge is limited regarding how neuromuscular training, conducted before and after soccer training, affects the fitness levels of youth soccer players. In this study, we aimed to analyze the effects of an eight-week neuromuscular training (NMT) program implemented before or after a soccer session on physical fitness in youth soccer players. Thirty-two youth soccer players were categorized into two groups—namely, NMT before soccer-specific training (NBS; n = 15) and NMT after soccer-specific training (NAS; n = 17). NMT comprised integrated resistance, dynamic stability, core, and plyometric training three times weekly and was conducted for 8 weeks. Before and after the exercise intervention, the counter-movement jump (CMJ) and 10 and 20 m sprint were analyzed, and the results of the T-agility test, Illinois change of direction test (ICDT), and Y-balance test were assessed for all participants. In terms of the interaction between the effects of the time of observation and group, both groups showed improvement in the results of the 10 m sprint and T-agility and Y-balance tests. Regarding the difference in the time of observation, the NBS group showed positive improvements in the results of the CMJ, 10 and 20 m sprints, and T-agility test after the exercise intervention, and the NAS group showed positive improvements in the results of the CMJ, 10 m sprint, T-agility test, and ICDT after the exercise intervention. These findings suggest that neuromuscular training has the potential to improve the 10 m sprint ability and T-agility test results of youth soccer players, regardless of the training sequence. Full article

Feline immunodeficiency virus (FIV) is responsible for immunodeficiency syndrome in cats. Several viral subtypes have been identified, each with a variable geographical distribution. To date, the subtype B is known to be the genotype spread in Italy. In this study, the genetic diversity of FIV in northern Italy was assessed by detecting proviral DNA in the blood samples of 50 cats determined to be positive through an anti-FIV antibodies test. These cats were tested using six different PCR assays, and the identified viruses were sequenced and analyzed. Forty-eight cats were confirmed positive, and several FIV subtypes were characterized. As expected, the subtype B was the most commonly observed, and the subtype A was reported for the first time in Italy. Moreover, a new taxon possibly representing an additional FIV subtype was detected, and one virus belonging to subtype B potentially had a recombinant origin. The genetic variability between the FIV viruses that emerged in this study may lead to the potential diagnostic failure of single molecular tests. Therefore, a new diagnostic strategy, which adopts different molecular tests and sequencing, is recommended to monitor the evolution and spread of FIV. Full article

This paper examines patterns of gemination in child Egyptian Arabic, with a focus on how gemination functions as a repair strategy, using data from the Egyptian Arabic Salama Corpus. The findings show that the phonological development of Egyptian Arabic-speaking children of geminated consonants correlates with previously established developmental stages. Initial stages involve the acquisition of labial geminates, transitioning through an increased use of alveolar and velar geminates, to the acquisition of rhotic and lateral geminates in later phases. The findings also suggest that gemination is not merely a phonetic phenomenon in child phonology, but also shows the children’s awareness of the phonology of the dialect, especially the moraicity of vowels and consonants. Full article

The purpose of this study is to explore the sustainability of island tourism by investigating the increasingly dangerous climate conditions that tourists encounter under climate change. The exploratory case of Hawaii, United States is offered to explore how thermal stress risks have (1) changed as a process of climate change and (2) are associated with airport arrivals of domestic tourists from states other than Hawaii (dependent variable). Hawaii is a destination-based island in the lower-latitudes of the Pacific Ocean that attracts more tourists than many stand-alone countries. Thermal stress—our independent variable—is a climate measure for heat exposure and has medically prescribed levels (°C) for heat-related risks to recreationalists and workers. The findings indicate that (1) thermal stress is increasing as a process of climate change over the past four decades (1984–2023), (2) moderate thermal stress risks are positively related to tourists’ demand (2015–2023), and (3) moderate thermal stress risks are more strongly related to tourists’ demand than lower risk levels. The results suggest that despite increased thermal stress, the demand for tourists’ arrivals remains sustainable for Hawaii. Though not significant, we also observed a decline in tourists’ arrivals with moderately high thermal stress risks. Implications are broken down for each of the sustainable tourism stakeholders: visitors, communities, industry, and the natural environment. Full article

Land-use change (LUC) is a significant contributor to the increase in atmospheric CO₂ concentrations, with previous studies demonstrating its profound impact on soil organic carbon (SOC). The conversion of primary forests to farmland has been recognized as the most significant type of LUC inducing CO₂ release from the soil. Therefore, it is critical to understand the impacts of forest LUC on SOC storage, with a particular focus on primary forest to farmland conversion. In this study, we conducted a meta-analysis of 411 observations from 41 published works and found that SOC storage decreased significantly following the conversion of primary forests to farmland. Factors such as soil depth and climate zone influenced the degree of SOC storage loss, with SOC loss being less severe in deeper soil following a conversion from primary forests to farmland. Moreover, the loss of SOC storage was more severe in temperate regions compared to tropical regions. The input and output of surface SOC, changes in soil structure, and increases in atmospheric CO₂ concentrations were significant reasons for the loss of SOC following primary forest to farmland LUC. However, improving tillage methods and implementing sustainable agricultural management strategies can help reduce SOC loss. These findings highlight the importance of sustainable land-use practices in mitigating the negative impacts of forest LUC on SOC storage and the global carbon cycle. Full article