The 2023 MDPI Annual Report has
been released!
 
11 pages, 2637 KiB  
Article
CXCR4 Expression as a Prognostic Biomarker in Soft Tissue Sarcomas
by Anna C. Virgili, Juliana Salazar, Alberto Gallardo, Antonio López-Pousa, Raúl Terés, Silvia Bagué, Ruth Orellana, Caterina Fumagalli, Ramon Mangues, Lorena Alba-Castellón, Ugutz Unzueta, Isolda Casanova and Ana Sebio
Diagnostics 2024, 14(11), 1195; https://doi.org/10.3390/diagnostics14111195 (registering DOI) - 6 Jun 2024
Abstract
Poor long-term survival in localized high-risk soft tissue sarcomas (STSs) of the extremities and trunk highlights the need to identify new prognostic factors. CXCR4 is a chemokine receptor involved in tumor progression, angiogenesis, and metastasis. The aim of this study was to evaluate [...] Read more.
Poor long-term survival in localized high-risk soft tissue sarcomas (STSs) of the extremities and trunk highlights the need to identify new prognostic factors. CXCR4 is a chemokine receptor involved in tumor progression, angiogenesis, and metastasis. The aim of this study was to evaluate the association between CXCR4 expression in tumor tissue and survival in STSs patients treated with neoadjuvant therapy. CXCR4 expression was retrospectively determined by immunohistochemical analysis in serial specimens including initial biopsies, tumors post-neoadjuvant treatment, and tumors after relapse. We found that a positive cytoplasmatic expression of CXCR4 in tumors after neoadjuvant treatment was a predictor of poor recurrence-free survival (RFS) (p = 0.003) and overall survival (p = 0.019) in synovial sarcomas. We also found that positive nuclear CXCR4 expression in the initial biopsies was associated with poor RFS (p = 0.022) in undifferentiated pleomorphic sarcomas. In conclusion, our study adds to the evidence that CXCR4 expression in tumor tissue is a promising prognostic factor for STSs. Full article
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12 pages, 728 KiB  
Review
Ineffective Learning Behaviors and Their Psychological Mechanisms among Adolescents in Online Learning: A Narrative Review
by Ji Li, Li Fang, Yu Liu, Jiayu Xie and Xiaoyu Wang
Behav. Sci. 2024, 14(6), 477; https://doi.org/10.3390/bs14060477 (registering DOI) - 6 Jun 2024
Abstract
During the COVID-19 pandemic, many countries and regions experienced a surge in online learning, but the public complained about and questioned its effectiveness. One of the most important reasons for this was the inadequate metacognitive abilities of adolescents. Studies in learning sciences have [...] Read more.
During the COVID-19 pandemic, many countries and regions experienced a surge in online learning, but the public complained about and questioned its effectiveness. One of the most important reasons for this was the inadequate metacognitive abilities of adolescents. Studies in learning sciences have identified various inefficient learning behaviors among students in online learning, including help abuse, help avoidance, and wheel spinning; all closely related to metacognition. Despite concerns about ecological validity, researchers in psychology have proposed the agenda-based regulation framework, the COPES model, and MAPS model, which may help explain the inefficient learning behaviors among adolescents in online learning. Future studies should aim to verify these theoretical frameworks within the context of online learning and elucidate the causes of inefficient learning behaviors; the design and optimization of online learning systems should be informed by theories in cognitive psychology. Full article
(This article belongs to the Special Issue Social Behaviors in Childhood: Causes and Consequences)
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14 pages, 2851 KiB  
Article
Determination of Saffron Flower Metabolites by Near-Infrared Spectroscopy for Quality Control
by Jorge F. Escobar-Talavera, María Esther Martínez-Navarro, Gonzalo L. Alonso and Rosario Sánchez-Gómez
Horticulturae 2024, 10(6), 593; https://doi.org/10.3390/horticulturae10060593 (registering DOI) - 6 Jun 2024
Abstract
Saffron, obtained by dehydrating the stigmas of the Crocus sativus flower, is a spice of great importance. In saffron, the flower emerges before leaf formation, thanks to the nutritional reserves of the corm. Early knowledge of metabolite levels such as crocins, picrocrocin, safranal, [...] Read more.
Saffron, obtained by dehydrating the stigmas of the Crocus sativus flower, is a spice of great importance. In saffron, the flower emerges before leaf formation, thanks to the nutritional reserves of the corm. Early knowledge of metabolite levels such as crocins, picrocrocin, safranal, anthocyanins, or kaempferols in flowers serves as a guide to evaluate the quality of the corm (coloring power, flavor, aroma, or antioxidant capacity, among others). In this study, near-infrared spectroscopy (NIR) was calibrated and validated to determine the main saffron metabolites, both in stigmas and in floral residue. To achieve this, saffron flowers from different locations of the Denomination of Origin (D.O.) “Azafrán de La Mancha” (Castilla-La Mancha, Spain) were analyzed using NIR spectroscopy. Prior to this, samples were analyzed by RP-HPLC-DAD, where the concentration of all cited metabolites was determined. The development of a predictive model through NIR calibration and validation was successful, achieving high R2 values, especially in the case of the sum of crocins and kaempferol-3-O-β-sophoroside. Using these predictive models, it is possible to determine the quality of saffron corm by analyzing the flower. Full article
(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)
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17 pages, 1177 KiB  
Article
Mapping Automated Cyber Attack Intelligence to Context-Based Impact on System-Level Goals
by Pete Burnap, Eirini Anthi, Philipp Reineckea, Lowri Williams, Fengnian Cao, Rakan Aldmoura and Kevin Jones
J. Cybersecur. Priv. 2024, 4(2), 340-356; https://doi.org/10.3390/jcp4020017 (registering DOI) - 6 Jun 2024
Abstract
Traditionally, cyber risk assessment considers system-level risk separately from individual component-level risk, i.e., devices, data, people. This separation prevents effective impact assessment where attack intelligence for a specific device can be mapped to its impact on the entire system, leading to cascading failures. [...] Read more.
Traditionally, cyber risk assessment considers system-level risk separately from individual component-level risk, i.e., devices, data, people. This separation prevents effective impact assessment where attack intelligence for a specific device can be mapped to its impact on the entire system, leading to cascading failures. Furthermore, risk assessments typically follow a failure or attack perspective, focusing on potential problems, which means they need to be updated as attacks evolve. This approach does not scale to modern digital ecosystems. In this paper, we present a Data Science approach, which involves using machine learning algorithms and statistical models to analyse and predict the impact of cyber attacks. Specifically, this approach integrates automated attack detection on specific devices with a systems view of risk. By mapping operational goals in a top-down manner, we transform attack intelligence on individual components into system success probabilities. Full article
(This article belongs to the Special Issue Data Protection and Privacy)
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12 pages, 2184 KiB  
Article
Short-Term Straw Return Combined with Nitrogen Fertilizer Alters the Soil Nitrogen Supply in Rice–Rapeseed Planting Systems
by Haicheng Wu, Zhi Zhang, Cheng Hu, Donghai Liu, Yan Qiao, Zhuoxi Xiao and Yupeng Wu
Agronomy 2024, 14(6), 1226; https://doi.org/10.3390/agronomy14061226 (registering DOI) - 6 Jun 2024
Abstract
This study aimed to assess the influences of short-term straw return combined with nitrogen (N) fertilizer on crop yield, soil properties, the bacterial community, and soil nitrogen cycling gene abundance in a rice–rapeseed planting system. A two-year field experiment was conducted in a [...] Read more.
This study aimed to assess the influences of short-term straw return combined with nitrogen (N) fertilizer on crop yield, soil properties, the bacterial community, and soil nitrogen cycling gene abundance in a rice–rapeseed planting system. A two-year field experiment was conducted in a paddy field from 2019 to 2021. There were four treatments in the experiment: −N−S, no N applied with no straw return; −N+S, no N applied with straw return; +N−S, N applied with no straw return; and +N+S, N applied with straw return. The results showed that short-term straw return combined with N fertilizer could increase crop yield and N use efficiency. N fertilizer application had a positive effect on Gemmatimonadota and Desulfobacterota abundance. Straw returning had a positive effect on Desulfobacterota and Proteobacteria abundance. N fertilization significantly increased the abundance of amoA-AOA, amoA-AOB, and nxrB in agricultural soils. Straw return and N fertilization were not conducive to denitrification. We conclude that short-term straw return combined with N fertilizer in rice-growing areas not only increase crop yield and improve crop N uptake but also increase SOM, total N, and NH4+ and improve the soil microbial activity and N use efficiency. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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15 pages, 273 KiB  
Article
Presence in the Dark: Joint Attention and the Varying Modes of Being Aware of God’s Presence
by Juan Camilo Espejo-Serna, John Anderson P-Duarte and Jorge Eduardo Arbeláez
Religions 2024, 15(6), 701; https://doi.org/10.3390/rel15060701 (registering DOI) - 6 Jun 2024
Abstract
This paper examines the phenomenon of joint attention and its relevance in understanding the modes of awareness of the presence of God. It explores the perspectives of Eleonore Stump and Andrew Pinsent, as well as the challenge raised by Donald Bungum, with the [...] Read more.
This paper examines the phenomenon of joint attention and its relevance in understanding the modes of awareness of the presence of God. It explores the perspectives of Eleonore Stump and Andrew Pinsent, as well as the challenge raised by Donald Bungum, with the aim of reaching a better understanding of a distinct way of being ‘moved by God in a divine way’. According to Stump and Pinsent, joint attention can deepen our understanding of our relationship with God, emphasizing the importance of sharing attention with Him and making better sense of the way humans may be moved in a divine way. However, Bungum challenges this view by suggesting the possibility of closeness without explicit awareness. The paper concludes by emphasizing the need for a nuanced understanding of awareness and the presence of God in our spiritual lives, for our connection with God can manifest in diverse ways different from traditional accounts of explicit awareness. We suggest that a view of joint attention centered in action may be more suitable for an attempt to make sense of what ‘to be moved in a divine way’ amounts to. Full article
(This article belongs to the Special Issue Aquinas and the Sciences: Exploring the Past, Present, and Future)
16 pages, 1639 KiB  
Article
Analysis of Chlorpyrifos Pesticide Residue in Locally Grown Cauliflower, Cabbage, and Eggplant Using Gas Chromatography–Mass Spectrometry (GC-MS) Technique: A Bangladesh Perspective
by Mysha Momtaz and Mohidus Samad Khan
Foods 2024, 13(11), 1780; https://doi.org/10.3390/foods13111780 (registering DOI) - 6 Jun 2024
Abstract
Pesticides are chemical substances used to kill or control various types of pests, which are hazardous for crops and animals. Pesticides may remain on or in foods after these are applied to crops. Pesticide residue in food has been a major global concern [...] Read more.
Pesticides are chemical substances used to kill or control various types of pests, which are hazardous for crops and animals. Pesticides may remain on or in foods after these are applied to crops. Pesticide residue in food has been a major global concern since there are direct and indirect health hazards associated with the regular consumption of foods with pesticide residues. Chlorpyrifos is one of the most used pesticides that has received much attention worldwide due to its detrimental health impact. The presence of chlorpyrifos residue in food crops can have both long-term and short-term effects on consumer health. Bangladesh is an agricultural country that uses a high volume of pesticides every year including chlorpyrifos. This experimental study aimed to analyze chlorpyrifos pesticide residue in locally grown cauliflower, cabbage, and eggplant samples by gas chromatography–mass spectrometry (GC-MS) technique followed by a suitable extraction process. Commercially available cauliflower, cabbage, and eggplant samples along with samples cultivated with the recommended pesticide dose were collected for qualitative and quantitative analysis. Samples cultivated without chlorpyrifos were collected as control samples for the validation study. The method was validated with respect to accuracy, recovery, reproducibility, linearity, limit of detection, and limit of quantification. The method has a limit of detection (LOD) of 0.011 mg/kg and a limit of quantification (LOQ) of 0.034 mg/kg. The experimental results were compared to the maximum residue level (MRL) to assess the human health impact. Chlorpyrifos residue was found in 44% of cauliflower samples with 91% of samples higher than MRL. The residue was found in 68% of cabbage samples with 53% of samples higher than MRL. For eggplant, the residue was found in 80% of the samples with 65% of samples higher than MRL. The risk assessment based on the residue level found in this study shows a potential health hazard of having a high concentration of chlorpyrifos residue in locally grown vegetables. Full article
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10 pages, 919 KiB  
Article
A Dynamical Analysis and New Traveling Wave Solution of the Fractional Coupled Konopelchenko–Dubrovsky Model
by Jin Wang and Zhao Li
Fractal Fract. 2024, 8(6), 341; https://doi.org/10.3390/fractalfract8060341 (registering DOI) - 6 Jun 2024
Abstract
The main object of this paper is to study the traveling wave solutions of the fractional coupled Konopelchenko–Dubrovsky model by using the complete discriminant system method of polynomials. Firstly, the fractional coupled Konopelchenko–Dubrovsky model is simplified into nonlinear ordinary differential equations by using [...] Read more.
The main object of this paper is to study the traveling wave solutions of the fractional coupled Konopelchenko–Dubrovsky model by using the complete discriminant system method of polynomials. Firstly, the fractional coupled Konopelchenko–Dubrovsky model is simplified into nonlinear ordinary differential equations by using the traveling wave transformation. Secondly, the trigonometric function solutions, rational function solutions, solitary wave solutions and the elliptic function solutions of the fractional coupled Konopelchenko–Dubrovsky model are derived by means of the polynomial complete discriminant system method. Moreover, a two-dimensional phase portrait is drawn. Finally, a 3D-diagram and a 2D-diagram of the fractional coupled Konopelchenko–Dubrovsky model are plotted in Maple 2022 software. Full article
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20 pages, 525 KiB  
Article
Middle Management Leadership Experiences of a Mission-Driven Innovation University Strategy
by Svend H. Christiansen, Xiangyun Du and Aida Olivia Pereira de Carvalho Guerra
Educ. Sci. 2024, 14(6), 609; https://doi.org/10.3390/educsci14060609 (registering DOI) - 6 Jun 2024
Abstract
This study explores academic middle managers’ perspectives on a mission-driven university strategy in a Danish context, offering insights for institutions implementing similar strategies. The case of a Danish university highlights the vital role of academic middle managers in navigating strategic uncertainty. Success requires [...] Read more.
This study explores academic middle managers’ perspectives on a mission-driven university strategy in a Danish context, offering insights for institutions implementing similar strategies. The case of a Danish university highlights the vital role of academic middle managers in navigating strategic uncertainty. Success requires a nuanced understanding of organizational dynamics and aligning initiatives with institutional goals. The study, based on interviews with ten academic middle managers, reveals complexities and challenges associated with mission-driven innovation in higher education, through a phenomenographic and thematic analysis, concerning current impressions and experiences, the perceived barriers to implementation, and the perceived affordances of transforming into a mission-driven university. The findings emphasize the significance of overcoming internal resistance and establishing institutional support, providing valuable considerations for higher education institutions and their academic middle managers in implementing mission-driven innovation strategies, and have the potential to support the design and implementation of future higher education strategies for sustainability, pertaining to the responsibilities, interactions, and mediations across and among the upper management and the academic staff. Full article
(This article belongs to the Section Higher Education)
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26 pages, 9798 KiB  
Article
Day of the Week Effect on the World Exchange Rates through Fractal Analysis
by Werner Kristjanpoller and Benjamin Miranda Tabak
Fractal Fract. 2024, 8(6), 340; https://doi.org/10.3390/fractalfract8060340 (registering DOI) - 6 Jun 2024
Abstract
The foreign exchange rate market is one of the most liquid and efficient. In this study, we address the efficient analysis of this market by verifying the day-of-the-week effect with fractal analysis. The presence of fractality was evident in the return series of [...] Read more.
The foreign exchange rate market is one of the most liquid and efficient. In this study, we address the efficient analysis of this market by verifying the day-of-the-week effect with fractal analysis. The presence of fractality was evident in the return series of each day and when analyzing an upward trend and a downward trend. The econometric models showed that the day-of-the-week effect in the studied currencies did not align with previous studies. However, analyzing the Hurst exponent of each day revealed that there a weekday effect in the fractal dimension. Thirty main world currencies from all continents were analyzed, showing weekday effects according to their fractal behavior. These results show a form of market inefficiency, as the returns or price variations of each day for the analyzed currencies should have behaved similarly and tended towards random walks. This fractal day-of-the-week effect in world currencies allows us to generate investment strategies and to better complement or support buying and selling decisions on certain days. Full article
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10 pages, 2627 KiB  
Article
Experimental and Theoretical Investigations of Direct and Indirect Band Gaps of WSe2
by Yingtao Wang and Xian Zhang
Micromachines 2024, 15(6), 761; https://doi.org/10.3390/mi15060761 (registering DOI) - 6 Jun 2024
Abstract
Low-dimension materials such as transition metal dichalcogenides (TMDCs) have received extensive research interest and investigation for electronic and optoelectronic applications. Due to their unique widely tunable band structures, they are good candidates for next-generation optoelectronic devices. Particularly, their photoluminescence properties, which are fundamental [...] Read more.
Low-dimension materials such as transition metal dichalcogenides (TMDCs) have received extensive research interest and investigation for electronic and optoelectronic applications. Due to their unique widely tunable band structures, they are good candidates for next-generation optoelectronic devices. Particularly, their photoluminescence properties, which are fundamental for optoelectronic applications, are highly sensitive to the nature of the band gap. Monolayer TMDCs in the room temperature range have presented a direct band gap behavior and bright photoluminescence. In this work, we investigate a popular TMDC material WSe2’s photoluminescence performance using a Raman spectroscopy laser with temperature dependence. With temperature variation, the lattice constant and the band gap change dramatically, and thus the photoluminescence spectra are changed. By checking the photoluminescence spectra at different temperatures, we are able to reveal the nature of direct-to-indirect band gap in monolayer WSe2. We also implemented density function theory (DFT) simulations to computationally investigate the band gap of WSe2 to provide comprehensive evidence and confirm the experimental results. Our study suggests that monolayer WSe2 is at the transition boundary between the indirect and direct band gap at room temperature. This result provides insights into temperature-dependent optical transition in monolayer WSe2 for quantum control, and is important for cultivating the potential of monolayer WSe2 in thermally tunable optoelectronic devices operating at room temperature. Full article
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16 pages, 2558 KiB  
Article
Investigation of Particle Rotation Characteristics and Compaction Quality Control of Asphalt Pavement Using the Discrete Element Method
by Zhi Zhang, Hancheng Dan, Hongyu Shan and Songlin Li
Materials 2024, 17(11), 2764; https://doi.org/10.3390/ma17112764 - 5 Jun 2024
Abstract
The compaction of asphalt pavement is a crucial step to ensure its service life. Although intelligent compaction technology can monitor compaction quality in real time, its application to individual asphalt surface courses still faces limitations. Therefore, it is necessary to study the compaction [...] Read more.
The compaction of asphalt pavement is a crucial step to ensure its service life. Although intelligent compaction technology can monitor compaction quality in real time, its application to individual asphalt surface courses still faces limitations. Therefore, it is necessary to study the compaction mechanism of asphalt pavements from the particle level to optimize intelligent compaction technology. This study constructed an asphalt pavement compaction model using the Discrete Element Method (DEM). First, the changes in pavement smoothness during the compaction process were analyzed. Second, the changes in the angular velocity of the mixture and the triaxial angular velocity (TAV) of the mortar, aggregates, and mixture during vibratory compaction were examined. Finally, the correlations between the TAV amplitude and the coordination number (CN) amplitude with the compaction degree of the mixture were investigated. This study found that vibratory compaction can significantly reduce asymmetric wave deformation, improving pavement smoothness. The mixture primarily rotates in the vertical plane during the first six passes of vibratory compaction and within the horizontal plane during the seventh pass. Additionally, TAV reveals the three-dimensional dynamic rotation characteristics of the particles, and the linear relationship between its amplitude and the pavement compaction degree aids in controlling the compaction quality of asphalt pavements. Finally, the linear relationship between CN amplitude and pavement compaction degree can predict the stability of the aggregate structure. This study significantly enhances quality control in pavement compaction and advances intelligent compaction technology development. Full article
(This article belongs to the Section Construction and Building Materials)
21 pages, 4973 KiB  
Article
Advanced Millimeter-Wave Radar System for Real-Time Multiple-Human Tracking and Fall Detection
by Zichao Shen, Jose Nunez-Yanez and Naim Dahnoun
Sensors 2024, 24(11), 3660; https://doi.org/10.3390/s24113660 - 5 Jun 2024
Abstract
This study explored an indoor system for tracking multiple humans and detecting falls, employing three Millimeter-Wave radars from Texas Instruments. Compared to wearables and camera methods, Millimeter-Wave radar is not plagued by mobility inconveniences, lighting conditions, or privacy issues. We conducted an initial [...] Read more.
This study explored an indoor system for tracking multiple humans and detecting falls, employing three Millimeter-Wave radars from Texas Instruments. Compared to wearables and camera methods, Millimeter-Wave radar is not plagued by mobility inconveniences, lighting conditions, or privacy issues. We conducted an initial evaluation of radar characteristics, covering aspects such as interference between radars and coverage area. Then, we established a real-time framework to integrate signals received from these radars, allowing us to track the position and body status of human targets non-intrusively. Additionally, we introduced innovative strategies, including dynamic Density-Based Spatial Clustering of Applications with Noise (DBSCAN) clustering based on signal SNR levels, a probability matrix for enhanced target tracking, target status prediction for fall detection, and a feedback loop for noise reduction. We conducted an extensive evaluation using over 300 min of data, which equated to approximately 360,000 frames. Our prototype system exhibited a remarkable performance, achieving a precision of 98.9% for tracking a single target and 96.5% and 94.0% for tracking two and three targets in human-tracking scenarios, respectively. Moreover, in the field of human fall detection, the system demonstrates a high accuracy rate of 96.3%, underscoring its effectiveness in distinguishing falls from other statuses. Full article
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22 pages, 30049 KiB  
Article
Alpha-Synuclein Gene Alterations Modulate Tyrosine Hydroxylase in Human iPSC-Derived Neurons in a Parkinson’s Disease Animal Model
by Luis Daniel Bernal-Conde, Verónica Peña-Martínez, C. Alejandra Morato-Torres, Rodrigo Ramos-Acevedo, Óscar Arias-Carrión, Francisco J. Padilla-Godínez, Alexa Delgado-González, Marcela Palomero-Rivero, Omar Collazo-Navarrete, Luis O. Soto-Rojas, Margarita Gómez-Chavarín, Birgitt Schüle and Magdalena Guerra-Crespo
Life 2024, 14(6), 728; https://doi.org/10.3390/life14060728 (registering DOI) - 5 Jun 2024
Abstract
Parkinson’s disease (PD) caused by SNCA gene triplication (3XSNCA) leads to early onset, rapid progression, and often dementia. Understanding the impact of 3XSNCA and its absence is crucial. This study investigates the differentiation of human induced pluripotent stem cell (hiPSC)-derived [...] Read more.
Parkinson’s disease (PD) caused by SNCA gene triplication (3XSNCA) leads to early onset, rapid progression, and often dementia. Understanding the impact of 3XSNCA and its absence is crucial. This study investigates the differentiation of human induced pluripotent stem cell (hiPSC)-derived floor-plate progenitors into dopaminergic neurons. Three different genotypes were evaluated in this study: patient-derived hiPSCs with 3XSNCA, a gene-edited isogenic line with a frame-shift mutation on all SNCA alleles (SNCA 4KO), and a normal wild-type control. Our aim was to assess how the substantia nigra pars compacta (SNpc) microenvironment, damaged by 6-hydroxydopamine (6-OHDA), influences tyrosine hydroxylase-positive (Th+) neuron differentiation in these genetic variations. This study confirms successful in vitro differentiation into neuronal lineage in all cell lines. However, the SNCA 4KO line showed unusual LIM homeobox transcription factor 1 alpha (Lmx1a) extranuclear distribution. Crucially, both 3XSNCA and SNCA 4KO lines had reduced Th+ neuron expression, despite initial successful neuronal differentiation after two months post-transplantation. This indicates that while the SNpc environment supports early neuronal survival, SNCA gene alterations—either amplification or knock-out—negatively impact Th+ dopaminergic neuron maturation. These findings highlight SNCA’s critical role in PD and underscore the value of hiPSC models in studying neurodegenerative diseases. Full article
(This article belongs to the Section Animal Science)
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16 pages, 9985 KiB  
Article
The Influence of Beachrock Outcrop on Spatial Variability in Beach Morphology
by Junhui Xu, Yanhong Wang and Peidong Lu
J. Mar. Sci. Eng. 2024, 12(6), 945; https://doi.org/10.3390/jmse12060945 - 5 Jun 2024
Abstract
Beachrock is a type of coastal carbonate sedimentary rock developed in the sandy beach intertidal zone, widely distributed along the beach front, and the loss of beach sediment is the main cause of beachrock exposure. Based on the analysis of measured data of [...] Read more.
Beachrock is a type of coastal carbonate sedimentary rock developed in the sandy beach intertidal zone, widely distributed along the beach front, and the loss of beach sediment is the main cause of beachrock exposure. Based on the analysis of measured data of different exposure forms of beachrocks in profiles, this paper analyzes the main features and influences of non-dynamic factors, such as the exposure position and morphology, of beachrocks on the dynamic geomorphic processes of beaches. Studies have shown that (1) changes between beach energy dissipation bodies are significant features of coastal geomorphic processes under the influence of beachrocks. The first spatial mode of EOF analysis shows that the erosion position of beach-rock-exposed profiles is mainly concentrated in the protected and real sections of beachrock, and the first temporal mode indicates that the exposure of beachrock results in a lagged response of its profile to dynamic environmental changes. (2) The differences in the exposure forms of beachrocks determine the sand-holding space of the beach, and the differences in the lateral exposure positions of beachrocks determine the main areas where erosion occurs on the profile. Meanwhile, the geomorphic processes of their profiles show different degrees of feedback, and such geomorphic phenomena can serve as reference indicators for the stage-wise evolution process influenced by beachrocks. Full article
(This article belongs to the Section Coastal Engineering)
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10 pages, 3413 KiB  
Case Report
Reconstruction of Bilateral Chronic Triceps Brachii Tendon Disruption Using a Suture-Mediated Anatomic Footprint Repair in a Dog
by Jong-Pil Yoon, Hae-Beom Lee, Young-Jin Jeon, Dae-Hyun Kim, Seong-Mok Jeong and Jae-Min Jeong
Animals 2024, 14(11), 1687; https://doi.org/10.3390/ani14111687 (registering DOI) - 5 Jun 2024
Abstract
A 2-year-old, intact female Pomeranian presented with bilateral forelimb lameness, characterized by the olecranon making contact with the ground. The patient experienced two separate incidents of falling, occurring four and three weeks before admission, respectively. Following each episode, non-weight-bearing lameness was initially observed [...] Read more.
A 2-year-old, intact female Pomeranian presented with bilateral forelimb lameness, characterized by the olecranon making contact with the ground. The patient experienced two separate incidents of falling, occurring four and three weeks before admission, respectively. Following each episode, non-weight-bearing lameness was initially observed in the left forelimb, followed by the development of crouch gait. Based on the physical examination, radiographic, and ultrasonographic findings, bilateral triceps brachii tendon disruption was diagnosed. Intraoperatively, excessive granulation tissue at the distal end of the tendon was excised. The footprint region of each triceps brachii tendon was decorticated with a high-speed burr until bleeding was observed. The triceps brachii tendon was reattached to completely cover its footprint on the olecranon using the Krackow suture technique. This method involves anchoring the suture through bone tunnels in the ulna. Trans-articular external skeletal fixation was applied to both forelimbs to immobile and stabilize the elbow joints for nine weeks. Subsequently, the dog gradually increased its walking activities while on a leash over a six-week period. At the three-year follow-up, the patient exhibited improved forelimb function and maintained a normal gait without signs of lameness. Suture-mediated anatomic footprint repair proved useful in this single case and may be an effective surgical alternative for the management of chronic triceps brachii tendon disruption in dogs. Full article
(This article belongs to the Special Issue Small Animal Orthopedic Surgery, Physical Therapy and Rehabilitation)
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16 pages, 4776 KiB  
Article
Two Coffee Diterpenes, Kahweol and Cafestol, Inhibit Extracellular Melanogenesis: An In Vitro Pilot Study
by Shilpi Goenka
Biologics 2024, 4(2), 202-217; https://doi.org/10.3390/biologics4020014 - 5 Jun 2024
Abstract
Hyperpigmentation skin disorders are marked by an abnormal accumulation or export of melanin pigment synthesized within melanocytes and pose a significant aesthetic concern. The search for novel natural compounds that exhibit pharmacological potential for treating pigmentation disorders is growing. In this study, kahweol [...] Read more.
Hyperpigmentation skin disorders are marked by an abnormal accumulation or export of melanin pigment synthesized within melanocytes and pose a significant aesthetic concern. The search for novel natural compounds that exhibit pharmacological potential for treating pigmentation disorders is growing. In this study, kahweol (KW) and cafestol (CFS), two structural analogs of coffee diterpenes, were evaluated and compared for their effects on melanogenesis using B16F10 mouse melanoma cells and primary human melanocytes derived from Asian and African American skin. To the best of our knowledge, there are no reports of the effects of KW and CFS on melanogenesis yet. We first screened nontoxic concentrations of both compounds using an MTS assay after 72 h incubations and subsequently tested their effects on melanin synthesis and export. Cellular tyrosinase activity and cell-free mushroom tyrosinase activity were assayed to study the mechanisms of melanogenesis suppression. Human melanocytes from a moderately pigmented donor (HEMn-MP cells) and from a darkly pigmented donor (HEMn-DP cells) were next examined, and effects on cellular viability, melanin content, cellular tyrosinase activity, and melanin export (quantitated via dendricity) were similarly examined for both compounds. Our results show that KW and CFS did not significantly affect intracellular melanin content but suppressed extracellular melanin in B16F10 cells and dendritic parameters in human melanocytes, indicating their unique capacity to target extracellular melanogenesis and melanin export. Although KW showed a greater extracellular melanogenesis inhibitory capacity in B16F10 cells, in both primary melanocyte cells, CFS emerged as a potent inhibitor of melanin export compared to KW. Together, these results reveal novel modes of action of both compounds and indicate a promise to use CFS as a novel candidate for treating hyperpigmentation disorders of the human skin for clinical and cosmetic use. Additional research is necessary to shed light on the molecular pathways and the efficacy of melanogenesis inhibition by CFS in 3D human skin equivalents and in vivo studies. Full article
(This article belongs to the Section Natural Products)
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13 pages, 515 KiB  
Review
Radiologists’ Communicative Role in Breast Cancer Patient Management: Beyond Diagnosis
by Luciano Mariano, Luca Nicosia, Adriana Sorce, Filippo Pesapane, Veronica Coppini, Roberto Grasso, Dario Monzani, Gabriella Pravettoni, Giovanni Mauri, Massimo Venturini, Maria Pizzamiglio and Enrico Cassano
Healthcare 2024, 12(11), 1145; https://doi.org/10.3390/healthcare12111145 (registering DOI) - 5 Jun 2024
Abstract
In the landscape of cancer treatment, particularly in the realm of breast cancer management, effective communication emerges as a pivotal factor influencing patient outcomes. This article delves into the nuanced intricacies of communication skills, specifically spotlighting the strategies embraced by breast radiologists. By [...] Read more.
In the landscape of cancer treatment, particularly in the realm of breast cancer management, effective communication emerges as a pivotal factor influencing patient outcomes. This article delves into the nuanced intricacies of communication skills, specifically spotlighting the strategies embraced by breast radiologists. By examining the ramifications of communication on patient experience, interdisciplinary collaboration, and legal ramifications, this study underscores the paramount importance of empathetic and comprehensive communication approaches. A special emphasis is placed on the utilization of the SPIKES protocol, a structured method for conveying sensitive health information, and the deployment of strategies for navigating challenging conversations. Furthermore, the work encompasses the significance of communication with caregivers, the integration of artificial intelligence, and the acknowledgement of patients’ psychological needs. By adopting empathetic communication methodologies and fostering multidisciplinary collaboration, healthcare practitioners have the potential to enhance patient satisfaction, promote treatment adherence, and augment the overall outcomes within breast cancer diagnosis. This paper advocates for the implementation of guidelines pertaining to psychological support and the allocation of sufficient resources to ensure the provision of holistic and patient-centered cancer care. The article stresses the need for a holistic approach that addresses patients’ emotional and psychological well-being alongside medical treatment. Through thoughtful and empathetic communication practices, healthcare providers can profoundly impact patient experiences and breast cancer journeys in a positive manner. Full article
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15 pages, 918 KiB  
Article
Social and Economic Determinants of Life Expectancy at Birth in Eastern Europe
by Viorel Țarcă, Elena Țarcă and Mihaela Moscalu
Healthcare 2024, 12(11), 1148; https://doi.org/10.3390/healthcare12111148 - 5 Jun 2024
Abstract
Life expectancy at birth is considered a parameter of the social development, health system, or economic development of a country. We aimed to investigate the effects of GDP per capita (as the economic factor), health care expenditure, the number of medical doctors (as [...] Read more.
Life expectancy at birth is considered a parameter of the social development, health system, or economic development of a country. We aimed to investigate the effects of GDP per capita (as the economic factor), health care expenditure, the number of medical doctors (as social factors), and CO2 emissions (as the environmental factor) on life expectancy. We used panel data analysis for 13 Eastern European countries over the 2000–2020 period. After performing the analysis, we used a cross-country fixed-effects panel (GLS with SUR weights). According to our model, a one percent increase in health expenditure (as % of GDP) increases life expectancy at birth by 0.376 years, whereas each additional medical doctor per 10,000 inhabitants increases life expectancy at birth by 0.088 years on average. At the same time, each additional 10,000 USD per capita each year would increase life expectancy at birth by 1.8 years on average. If CO2 emissions increase by 1 metric ton per capita, life expectancy at birth would decrease by 0.24 years, suggesting that higher carbon emissions are capable of reducing longevity. Every European country has to make special efforts to increase the life expectancy of its inhabitants by applying economic and health policies focused on the well-being of the population. Full article
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10 pages, 973 KiB  
Brief Report
Transcriptomic Profile of Mouse Brain Ageing in Early Developmental Stages
by Karolina Kulis, Kevin Tabury, Mohammed Abderrafi Benotmane and Joanna Polanska
Brain Sci. 2024, 14(6), 581; https://doi.org/10.3390/brainsci14060581 (registering DOI) - 5 Jun 2024
Abstract
Ageing is a continuous process that can cause neurodevelopmental changes in the body. Several studies have examined its effects, but few have focused on how time affects biological processes in the early stages of brain development. As studying the changes that occur in [...] Read more.
Ageing is a continuous process that can cause neurodevelopmental changes in the body. Several studies have examined its effects, but few have focused on how time affects biological processes in the early stages of brain development. As studying the changes that occur in the early stages of life is important to prevent age-related neurological and psychiatric disorders, we aim to focus on these changes. The transcriptomic markers of ageing that are common to the analysed brain regions of C57Bl/6J mice were identified after conducting two-way ANOVA tests and effect size analysis on the time courses of gene expression profiles in various mouse brain regions. A total of 16,374 genes (59.9%) significantly changed their expression level, among which 7600 (27.8%) demonstrated tissue-dependent differences only, and 1823 (6.7%) displayed time-dependent and tissue-independent responses. Focusing on genes with at least a large effect size gives the list of potential biomarkers 12,332 (45.1%) and 1670 (6.1%) genes, respectively. There were 305 genes that exhibited similar significant time response trends (independently of the brain region). Samples from an 11-day-old mouse embryo validated the identified early-stage brain ageing markers. The overall functional analysis revealed tRNA and rRNA processing in the mitochondrion and contact activation system (CAS), as well as the kallikrein/kinin system (KKS), together with clotting cascade and defective factor F9 activation being affected by ageing. Most ageing-related pathways were significantly enriched, especially those that are strongly connected to development processes and neurodegenerative diseases. Full article
(This article belongs to the Section Developmental Neuroscience)
18 pages, 3890 KiB  
Article
Conditional Knockout of Neurexins Alters the Contribution of Calcium Channel Subtypes to Presynaptic Ca2+ Influx
by Johannes Brockhaus, Iris Kahl, Mohiuddin Ahmad, Daniele Repetto, Carsten Reissner and Markus Missler
Cells 2024, 13(11), 981; https://doi.org/10.3390/cells13110981 - 5 Jun 2024
Abstract
Presynaptic Ca2+ influx through voltage-gated Ca2+ channels (VGCCs) is a key signal for synaptic vesicle release. Synaptic neurexins can partially determine the strength of transmission by regulating VGCCs. However, it is unknown whether neurexins modulate Ca2+ influx via all VGCC [...] Read more.
Presynaptic Ca2+ influx through voltage-gated Ca2+ channels (VGCCs) is a key signal for synaptic vesicle release. Synaptic neurexins can partially determine the strength of transmission by regulating VGCCs. However, it is unknown whether neurexins modulate Ca2+ influx via all VGCC subtypes similarly. Here, we performed live cell imaging of synaptic boutons from primary hippocampal neurons with a Ca2+ indicator. We used the expression of inactive and active Cre recombinase to compare control to conditional knockout neurons lacking either all or selected neurexin variants. We found that reduced total presynaptic Ca2+ transients caused by the deletion of all neurexins were primarily due to the reduced contribution of P/Q-type VGCCs. The deletion of neurexin1α alone also reduced the total presynaptic Ca2+ influx but increased Ca2+ influx via N-type VGCCs. Moreover, we tested whether the decrease in Ca2+ influx induced by activation of cannabinoid receptor 1 (CB1-receptor) is modulated by neurexins. Unlike earlier observations emphasizing a role for β-neurexins, we found that the decrease in presynaptic Ca2+ transients induced by CB1-receptor activation depended more strongly on the presence of α-neurexins in hippocampal neurons. Together, our results suggest that neurexins have unique roles in the modulation of presynaptic Ca2+ influx through VGCC subtypes and that different neurexin variants may affect specific VGCCs. Full article
(This article belongs to the Special Issue Diving Deep into Synaptic Transmission)
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10 pages, 567 KiB  
Article
Prevalence of Genetic Mutations in Patients with Metastatic Prostate Cancer in a Cohort of Mexican Patients
by Orión Erenhú Rodríguez González, Edgar Iván Bravo Castro, Jesus Eduardo Osorio, Habiram Pacheco Guerrero, Brenda Suaste Carmona, Luis David Arreola Peralta, Noe Esaul Martinez Juárez, Juan Samuel Izquierdo Luna, José de Jesús Oswaldo Islas García, Omar Dimas Victorio Vargas, Rafael Alberto Valdez Flores, Jesús Javier Torres Gómez and José Gadú Campos Salcedo
Soc. Int. Urol. J. 2024, 5(3), 172-181; https://doi.org/10.3390/siuj5030027 - 5 Jun 2024
Abstract
Background: Prostate cancer is a malignant neoplasm of the male genitourinary system with the highest incidence worldwide. Susceptibility genes related to aggressiveness and prognosis, such as BRCA1/2, ATM, PTEN, have been identified. Currently, reports related to germline mutations in patients with prostate cancer [...] Read more.
Background: Prostate cancer is a malignant neoplasm of the male genitourinary system with the highest incidence worldwide. Susceptibility genes related to aggressiveness and prognosis, such as BRCA1/2, ATM, PTEN, have been identified. Currently, reports related to germline mutations in patients with prostate cancer in Latin American populations are very limited or absent. In the Mexican population, reports are also limited, especially in the context of metastatic prostate cancer. Determining the prevalence of these mutations is relevant to predict the potential aggressiveness of tumors and allow the use of targeted therapies, such as PARPi inhibitors. Objective: Determine the prevalence of germline mutations in patients with metastatic prostate cancer and establish their clinical characteristics at diagnosis. Material and Methods: Sixty-nine patients with metastatic PCa underwent testing and genetic analysis using the Comprehensive Multi-Cancer Hereditary Cancer Panel. The prevalence of germline mutations was assessed, and the cohort was divided into two groups for the evaluation and analysis of clinical characteristics between the mutated and non-mutated populations. Results: We identified mutations in 15 out of 69 patients (21.73%), while 54 patients (78.26%) had no mutations. Pathogenic mutations were observed in 15.9% of patients, Variants of Uncertain Significance (VUS) in 34.78%, and 5.79% had both. The most frequent mutations included ATM (11.54%), BRCA1 (11.54%), BRCA2 (7.69%), FANCA (7.69%), and FANCM (7.69%). No statistically significant differences were found in PSA levels, age at diagnosis, and resistance to castration between the two groups. Conclusions: Our study unveiled a mutation rate of 21.73%, marked by a significant prevalence of ATM, FANCA, FANCM, and Variants of Uncertain Significance (VUS). This pattern deviates from findings in other series, underscoring the necessity for improved access to clinical genetic testing in our population. Full article
29 pages, 2444 KiB  
Review
Integrating OLAP with NoSQL Databases in Big Data Environments: Systematic Mapping
by Diana Martinez-Mosquera, Rosa Navarrete, Sergio Luján-Mora, Lorena Recalde and Andres Andrade-Cabrera
Big Data Cogn. Comput. 2024, 8(6), 64; https://doi.org/10.3390/bdcc8060064 (registering DOI) - 5 Jun 2024
Abstract
The growing importance of data analytics is leading to a shift in data management strategy at many companies, moving away from simple data storage towards adopting Online Analytical Processing (OLAP) query analysis. Concurrently, NoSQL databases are gaining ground as the preferred choice for [...] Read more.
The growing importance of data analytics is leading to a shift in data management strategy at many companies, moving away from simple data storage towards adopting Online Analytical Processing (OLAP) query analysis. Concurrently, NoSQL databases are gaining ground as the preferred choice for storing and querying analytical data. This article presents a comprehensive, systematic mapping, aiming to consolidate research efforts related to the integration of OLAP with NoSQL databases in Big Data environments. After identifying 1646 initial research studies from scientific digital repositories, a thorough examination of their content resulted in the acceptance of 22 studies. Utilizing the snowballing technique, an additional three studies were selected, culminating in a final corpus of twenty-five relevant articles. This review addresses the growing importance of leveraging NoSQL databases for OLAP query analysis in response to increasing data analytics demands. By identifying the most commonly used NoSQL databases with OLAP, such as column-oriented and document-oriented, prevalent OLAP modeling methods, such as Relational Online Analytical Processing (ROLAP) and Multidimensional Online Analytical Processing (MOLAP), and suggested models for batch and real-time processing, among other results, this research provides a roadmap for organizations navigating the integration of OLAP with NoSQL. Additionally, exploring computational resource requirements and performance benchmarks facilitates informed decision making and promotes advancements in Big Data analytics. The main findings of this review provide valuable insights and updated information regarding the integration of OLAP cubes with NoSQL databases to benefit future research, industry practitioners, and academia alike. This consolidation of research efforts not only promotes innovative solutions but also promises reduced operational costs compared to traditional database systems. Full article
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