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Signal generation and mixing electronics for frequency-domain lifetime and spectral fluorometryThe present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals. The processor then extracts the fluorescence lifetime and fluorescence spectrum of the emitted signal from the phase and amplitude information using a chemometric analysis.
Document ID
20080004472
Acquisition Source
Langley Research Center
Document Type
Other - Patent
Authors
Cruce, Tommy C.
Hallidy, William H.
Chin, Robert C.
Date Acquired
August 24, 2013
Publication Date
November 9, 1999
Subject Category
Instrumentation And Photography
Funding Number(s)
CONTRACT_GRANT: NAS1-20426
CONTRACT_GRANT: NAS1-20162
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Patent
US-PATENT-5,981,957
Patent Application
US-PATENT-APPL-SN-958779

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