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Journal Article

From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses

Authors
/persons/resource/enwosu

Nwosu,  Ebuka Canisius
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

Roeser,  Patricia
External Organizations;

/persons/resource/syang

Yang,  Sizhong
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/lganzert

Ganzert,  L.
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

Dellwig,  Olaf
External Organizations;

/persons/resource/sytopi

Pinkerneil,  Sylvia
4.3 Climate Dynamics and Landscape Evolution, 4.0 Geosystems, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/brau

Brauer,  A.
4.3 Climate Dynamics and Landscape Evolution, 4.0 Geosystems, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

Dittmann,  Elke
External Organizations;

/persons/resource/dwagner

Wagner,  D.
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

/persons/resource/sliebner

Liebner,  Susanne
3.7 Geomicrobiology, 3.0 Geochemistry, Departments, GFZ Publication Database, Deutsches GeoForschungsZentrum;

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Fulltext (public)

5007539.pdf
(Publisher version), 5MB

Supplementary Material (public)
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Citation

Nwosu, E. C., Roeser, P., Yang, S., Ganzert, L., Dellwig, O., Pinkerneil, S., Brauer, A., Dittmann, E., Wagner, D., Liebner, S. (2021): From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses. - Microorganisms, 9, 8, 1778.
https://doi.org/10.3390/microorganisms9081778


Cite as: https://gfzpublic.gfz-potsdam.de/pubman/item/item_5007539
Abstract
Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from sediments is crucial, especially in lakes lacking long-term monitoring data. To extend the resolution of sediment record interpretation, we used high-throughput sequencing, amplicon sequence variant (ASV) analysis, and quantitative PCR to compare pelagic cyanobacterial composition to that in sediment traps (collected monthly) and surface sediments in Lake Tiefer See. Cyanobacterial composition, species richness, and evenness was not significantly different among the pelagic depths, sediment traps and surface sediments (p > 0.05), indicating that the cyanobacteria in the sediments reflected the cyanobacterial assemblage in the water column. However, total cyanobacterial abundances (qPCR) decreased from the metalimnion down the water column. The aggregate-forming (Aphanizomenon) and colony-forming taxa (Snowella) showed pronounced sedimentation. In contrast, Planktothrix was only very poorly represented in sediment traps (meta- and hypolimnion) and surface sediments, despite its highest relative abundance at the thermocline (10 m water depth) during periods of lake stratification (May–October). We conclude that this skewed representation in taxonomic abundances reflects taphonomic processes, which should be considered in future DNA-based paleolimnological investigations. View Full-Text