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The yeast tRNATyr gene intron is essential for correct modification of its tRNA product

Nature volume 302, pages 681–687 (1983)Cite this article

Abstract

Precise deletion of the intervening sequence of a yeast tRNATyr ochre suppressor gene (SUP6) significantly reduced its suppressor activity relative to that of the unaltered gene. This is probably the result of the absence of the pseudouridine modification, normally present at the middle anticodon position of mature suppressor tRNA, in tRNA synthesized in vivo from the deleted gene.

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Author information

Author notes

  1. John Abelson

    Present address: Division of Biology, California Institute of Technology, Pasadena, California, 91125, USA

Authors and Affiliations

  1. Department of Biology, University of California at San Diego, La Jolla, California, 92093, USA

    Peter F. Johnson

  2. Department of Chemistry, University of California at San Diego, La Jolla, California, 92093, USA

    John Abelson

  3. The Agouron Institute, 505 Coast Boulevard South, La Jolla, California, 92037, USA

    John Abelson

Authors
  1. Peter F. Johnson
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  2. John Abelson
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Johnson, P., Abelson, J. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product. Nature 302, 681–687 (1983). https://doi.org/10.1038/302681a0

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