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Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes

Nature volume 306, pages 557–561 (1983)Cite this article

Abstract

DNA sequences containing the 5′-flanking regions of the insulin and chymotrypsin genes were linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. The insulin gene recombinant elicits preferential expression of CAT activity when introduced into cells producing insulin; similarly, the chymotrypsin gene recombinant elicits preferential expression in chymotrypsin-producing cells. Sequences located upstream of previously defined transcriptional control elements are essential for efficient expression in both cases.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, California, 94143, USA

    Michael D. Walker, Thomas Edlund, Anne M. Boulet & William J. Rutter

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  1. Michael D. Walker
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  2. Thomas Edlund
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  3. Anne M. Boulet
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  4. William J. Rutter
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Walker, M., Edlund, T., Boulet, A. et al. Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes. Nature 306, 557–561 (1983). https://doi.org/10.1038/306557a0

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