Abstract
We describe a technique, genetically directed representational difference analysis (GDRDA), for specifically generating genetic markers linked to a trait of interest. GDRDA is applicable, in principle, to virtually any organism, because it requires neither prior knowledge of the chromosomal location of the gene controlling the trait nor the availability of a pre–existing genetic map. Based on a subtraction technique described recently called representational difference analysis, GDRDA uses the principles of transmission genetics to create appropriate Tester and Driver samples for subtraction. We demonstrate the usefulness of GDRDA by, for example, successfully targeting three polymorphisms to an interval of less than 1 cM of the mouse nude locus of chromosome 11.
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References
Collins, F. Positional cloning: let's not call it reverse anymore. Nature Genet. 1, 3–6 (1992).
Lisitsyn, N., Lisitsyn, N. & Wigler, M. Cloning the difference between two complex genomes. Science 259, 946–951 (1993).
Snell, G.D. Histocompatibility genes of the mouse. II. Production and analysis of isogenic resistant lines. J. natn. Cancer Inst. 21, 843–877 (1958).
Hillyard, A.L., Doolittle, D.P., Davisson, M.T., Maltais, L. & Roderick, T.H. Locus map of the mouse with comparative map points of human on mouse. (GBASE Electronic Database, Jackson Laboratory, Bar Harbor, Maine, March, 1993).
Williams, J.G.K., Reiter, R.S., Young, R.M. & Scolnick, P.A. Genetic Mapping of mutations using phenotypic pools and mapped RAPD markers. Nucl. Adds Res. 21, 2697–2702 (1993).
Lyon, M.F. & Searle, A.G. Genetic Variants and Strains of the Laboratory Mouse 2nd edn (Oxford Univeristy Press, Oxford, 1989).
Brown, T. in Current Protocols in Molecular Biology (eds Ausubel, F. et al.) 2.9.1–2.9.15 (Green, New York, 1993).
Dietrich, W. et al. A Genetic Map of the Mouse Suitable for Typing lntraspecific Crosses. Genetics 131, 423–447 (1992).
Whitehead Institute/MIT Center for Genome Research, Mouse Genome Public Electronic Database, “genome_database@genome.wi.mit.edu” (July, (1993).
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Lisitsyn, N., Segre, J., Kusumi, K. et al. Direct isolation of polymorphic markers linked to a trait by genetically directed representational difference analysis. Nat Genet 6, 57–63 (1994). https://doi.org/10.1038/ng0194-57
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DOI: https://doi.org/10.1038/ng0194-57
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