Summary
Several racemates has been resolved according to an enzymatic recognition mechanism on an α-chymotrypsin chiral stationary phase. The loadability of the ACHT-CSP has been evaluated both in buffered and non-buffered mobile phases under the enzymatic process and compared with the loading capacity obtained by the structural recognition process.
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Félix, G., Descorps, V. Stereochemical resolution of racemates, in HPLC, using a chiral stationary phase based upon immobilized α-chymotrypsin. II. Enzyme chiral separations. Chromatographia 49, 606–614 (1999). https://doi.org/10.1007/BF02466901
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DOI: https://doi.org/10.1007/BF02466901