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Analysis ofAllium sulfur amino acids by HPLC after derivatization

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Summary

Two pre-column derivatization procedures coupled with reversed phase HPLC have been compared for the analysis ofS-Alk(en)yl0L-cysteine sulfoxides in variousAllium species. In order to establish external standards some (+/-) sulfoxides were synthesized, using a new method to enhance asymmetric synthesis of the diastereoisomers. The first derivatization method is the formation ofo-phthaldialdehyde/tert.-butylthiol derivatives which can be analyzed using UV detection. The second, presently used for amino acid analysis, is the Waters Pico-Tag method, which employs phenylisothiocyanate as derivatization reagent. As the Pico-Tag method was found to be the most efficient for determination ofS-alk(en)yl-L-cysteine sulfoxides it was used to determine the alliin content of various samples of garlic.

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Authors and Affiliations

  1. Institut de Biocénotique Expérimentale des Agrosystèmes, Université François Rabelais (URA CNRS 1298), Avenue Monge, Parc de Grandmont, 37200, Tours, France

    J. Auger, J. Boscher & N. Mandon

  2. Laboratoire de Botanique et Phytochimie, Faculté de Pharmacie, 92296, Châtenay-Malabry, France

    F. Mellouki, A. Vannereau & L. Cosson

Authors
  1. J. Auger
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  2. F. Mellouki
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  3. A. Vannereau
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  4. J. Boscher
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  5. L. Cosson
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  6. N. Mandon
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Auger, J., Mellouki, F., Vannereau, A. et al. Analysis ofAllium sulfur amino acids by HPLC after derivatization. Chromatographia 36, 347–350 (1993). https://doi.org/10.1007/BF02263889

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  • DOI: https://doi.org/10.1007/BF02263889

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