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Laser temperature jump experiments with fluorescence polarization and turbidity detection on the phase transition of single shell vesicles of dimyristoylphosphatidylcholine

  • Colloid Science
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Abstract

The iodine-laser temperature-jump technique has been used to investigate the main phase transition in single shell vesicles of dimyristoylphosphatidylcholine. The probe molecules DPH and TMA-DPH were incorporated into the lipid bilayer and laserT-jump experiments with turbidity and flourescence polarization detection were performed. We found three well separated relaxation processes between 5 μs and 10 ms. The relaxation signals showed strong cooperativity in the relaxation times as well as in their corresponding amplituedes. We attributed the relaxation to the formation and dissolution of clusters of different order inside the bilayer.

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Author notes

  1. A. Genz

    Present address: Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, F.R.G.

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  1. Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, F.R.G.

    J. F. Holzwarth

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  1. A. Genz
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  2. J. F. Holzwarth
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Genz, A., Holzwarth, J.F. Laser temperature jump experiments with fluorescence polarization and turbidity detection on the phase transition of single shell vesicles of dimyristoylphosphatidylcholine. Colloid & Polymer Sci 263, 484–493 (1985). https://doi.org/10.1007/BF01458339

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  • DOI: https://doi.org/10.1007/BF01458339

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