Skip to main content
SpringerLink
Log in

Morphogenetic plastid migration and microtubule organization during megasporogenesis inIsoetes

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The large megasporocytes ofIsoetes provide an exceptional system for studying microtubule dynamics in monoplastidic meiosis where plastid polarity assures coordination of plastid and nuclear division by the intimate association of MTOCs with plastids. Division and migration of the plastid in prophase establishes the tetrahedrally arranged cytoplasmic domains of the future spore tetrad and the four plastid-MTOCs serve as focal points of a unique quadripolar microtubule system (QMS). The QMS is a dynamic structure which functions in plastid deployment and contributes directly to development of both first and second division spindles. The nucleation of microtubules at discrete plastid-MTOCs is compared with centrosomal nucleation of microtubules in animal cells where growth of microtubules involves dynamic instability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AMS:

axial microtubule system

MTOC:

microtubule organizing center

N:

nucleus

QMS:

quadripolar microtubule system

P:

plastid

PPB:

preprophase band of microtubules

References

  • Brown RC, Lemmon BE (1982 a) Ultrastructure of meiosis in the mossRhynchostegium serrulatum I. Prophasic microtubules and spindle dynamics. Protoplasma 110: 23–33

    Google Scholar 

  • — — (1982 b) Ultrastructural aspects of moss meiosis: review of nuclear and cytoplasmic events during prophase. J Hattori Bot Lab 53: 29–39

    Google Scholar 

  • — — (1985 a) Development of stomata inSelaginella: division polarity and plastid movements. Am J Bot 72: 1914–1925

    Google Scholar 

  • — — (1985 b) Preprophasic establishment of division polarity in monoplastidic mitosis of hornworts. Protoplasma 124: 175–183

    Google Scholar 

  • — — (1985 c) A cytoskeletal system predicts division plane in meiosis ofSelaginella. Protoplasma 127: 101–109

    Google Scholar 

  • — — (1986) Three-dimensional relationship of the meiotic spindle to predivision microtubules inSphagnum. J Cell Biol 103 5,2: 55 a

    Google Scholar 

  • — — (1987 a) Division polarity, development and configuration of microtubule arrays in bryophyte meiosis I. Meiotic prophase to metaphase I. Protoplasma 137: 84–99

    Google Scholar 

  • — — (1987 b) Division polarity, development and configuration of microtubule arrays in bryophyte meiosis II. Anaphase I to the tetrad. Protoplasma 138: 1–10

    Google Scholar 

  • — — (1988) Preprophasic microtubule systems and development of the mitotic spindle in hornworts (Bryophyta). Protoplasma 143: 11–21

    Google Scholar 

  • Busby CH, Gunning BES (1988 a) Establishment of plastid-based quadripolarity in spore mother cells of the mossFunaria hygrometrica. J Cell Sci 91: 117–126

    Google Scholar 

  • — — (1988 b) Development of the quadripolar meiotic cytoskeleton in spore mother cells of the mossFunaria hygrometrica. J Cell Sci 91: 127–137

    Google Scholar 

  • — — (1989) Development of the quadripolar meiotic apparatus inFunaria spore mother cells: analysis by means of anti-microtubule drug treatments. J Cell Sci 93: 267–277

    Google Scholar 

  • Cassimeris L, Walker RA, Pryer NK, Salmon ED (1987) Dynamic instability of microtubules. BioEssays 7: 149–154

    Google Scholar 

  • Clayton L, Lloyd CW (1984) The relationship between the division plane and spindle geometry inAllium cells treated with CIPC and griseofulvin: an anti-tubulin study. Eur J Cell Biol 34: 248–253

    Google Scholar 

  • —, Black CM, Lloyd CW (1985) Microtubule nucleating sites in higher plant cells identified by an auto-antibody against pericentriolar material. J Cell Biol 101: 319–324

    Google Scholar 

  • De Mey J, Lambert A-M, Bajer AS, Moeremans M, De Brabander M (1982) The visualization of microtubules in interphase and mitotic plant cells ofHaemanthus endosperm with the immunogold staining methods. Proc Natl Acad Sci USA 79: 1898–1902

    Google Scholar 

  • Dunlop D (1949) Notes on the cytology of some lycopsids. Bull Torrey Bot Club 76: 266–277

    Google Scholar 

  • Fitting H (1900) Bau- und Entwickelungsgeschichte der Makrosporen vonIsoetes undSelaginella und ihre Bedeutung usw. Bot Z 58: 107–165

    Google Scholar 

  • Grolig F, Wagner G (1987) Light-dependent chloroplast reorientations inMougeotia andMesotaenium: biased by pigment-regulated plasmalemma anchorage sites to actin filaments? Bot Acta 101: 2–6

    Google Scholar 

  • Gunning BES (1982) The cytokinetic apparatus: its development and spatial regulation. In: Lloyd CW (ed) Cytoskeleton in plant growth and development. Academic Press, London, pp 229–292

    Google Scholar 

  • Haupt W (1982) Light-mediated movement of chloroplasts. Ann Rev Plant Physiol 33: 205–233

    Google Scholar 

  • Hogan CJ (1987) Microtubule patterns during meiosis in two higher plant series. Protoplasma 138: 126–136

    Google Scholar 

  • Lloyd CW, Pearce KJ, Rawlins DJ, Ridge RW, Shaw PJ (1987) Endoplasmic microtubules connect the advancing nucleus to the tip of legume root hairs, but F-actin is involved in basipetal migration. Cell Motil Cytoskel 8: 27–36

    Google Scholar 

  • Mazia D (1984) Centrosomes and mitotic poles. Exp Cell Res 153: 1–15

    Google Scholar 

  • Menzel D, Schliwa M (1986) Motility in the siphonous green algaBryopsis. II. Chloroplast movement requires organized arrays of both microtubules and actin filaments. Eur J Cell Biol 40: 286–295

    Google Scholar 

  • Schmit AC, Vantard M, De Mey J, Lambert A-M (1983) Aster-like microtubule centers establish spindle polarity during interphasemitosis transition in higher plant cells. Pl Cell Rep 2: 285–288

    Google Scholar 

  • Serlin BS, Ferrell S (1989) The involvement of microtubules in chloroplast rotation in the algaMougeotia. Plant Sci 60: 1–8

    Google Scholar 

  • Sorsa V (1955) Outlines of meiosis in the moss genusSphagnum. Hereditas 41: 250–258

    Google Scholar 

  • — (1956) The quadripolar spindle and the change of orientation of the chromosomes in meiosis ofSphagnum. Ann Acad Sci Fenn [A] 33: 1–64

    Google Scholar 

  • Wick SM (1985 a) The higher plant mitotic apparatus: redistribution of microtubules, calmodulin and microtubule initiation material during its establishment. Cytobios 43: 285–294

    Google Scholar 

  • — (1985 b) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. III. Transition between mitotic/cytokinetic and interphase microtubule arrays. Cell Biol Int Rept 9: 357–371

    Google Scholar 

  • —, Duniec J (1983) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Pre-prophase band development and concomitant appearance of nuclear envelope-associated tubulin. J Cell Biol 97: 235–243

    Google Scholar 

  • — — (1984) Immunofluorescence microscopy of tubulin and microtubule arrays in plants cells. II. Transition between the pre-prophase band and the mitotic spindle. Protoplasma 122: 45–55

    Google Scholar 

  • Witztum A, Parthasarathy MV (1985) Role of actin in chloroplast clustering and banding in leaves ofEgeria, Elodea andHydrilla. Eur J Cell Biol 39: 21–26

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, The University of Southwestern Louisiana, Lafayette, Louisiana

    R. C. Brown & B. E. Lemmon

Authors
  1. R. C. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. E. Lemmon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, R.C., Lemmon, B.E. Morphogenetic plastid migration and microtubule organization during megasporogenesis inIsoetes . Protoplasma 152, 136–147 (1989). https://doi.org/10.1007/BF01323073

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01323073

Keywords

Search

Navigation