Summary
An ultrastructural investigation of the monoplastidic microsporocytes ofSelaginella arenicola revealed a unique cytoskeletal array that predicts the future division plane before nuclear division takes place. By midprophase of the first meiotic division, the single plastid has divided once and the two plastids lie on opposite sides of the nucleus which is elongated in the plane of the incipient metaphase I spindle. A cytoplasmic structure, the procytokinetic plate (PCP), predicts the division plane of of both plastid and cytoplasm. The PCP consists of a distinct concentration of vesicles lying in the future division plane and an elaborate system of microtubules aligned parallel to the long axis of plastids and nucleus. Microtubules of the axially aligned system appear to terminate in clusters of vesicles in the central zone of the PCP. The PCP with axially aligned microtubules is as predictive of the division plane in these meiotic cells as is the girdling preprophase band of microtubules in mitotic cells.
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Brown, R. C., Lemmon, B. E., 1982 a: Ultrastructure of meiosis in the mossRhynchostegium serrulatum. I. Prophasic microtubules and spindle dynamics. Protoplasma110, 23–33.
— —, 1982 b: Ultrastructural aspects of moss meiosis: Review of nuclear and cytoplasmic events during prophase. J. Hattori Bot. Lab.53, 29–39.
— —, 1984: Plastid apportionment and preprophase microtubule bands in monoplastidic root meristem cells ofIsoetes andSelaginella. Protoplasma123, 95–103.
— —, 1985: Preprophasic establishment of division polarity in monoplastidic mitosis of hornworts. Protoplasma124, 175–183.
Buchen, B., Sievers, A., 1981: Sporogenesis and pollen grain formation. In: Cytomorphogenesis in plants (Kiermayer, O., ed.), pp. 349–376. Wien-New York: Springer.
Butterfass, T., 1979: Patterns of chloroplast reproduction. Wien-New York: Springer.
Dill, F. J., 1964: Dictyotene stage of meiosis in mosses. Science144, 541–543.
Esau, K., Gill, R. H., 1969: Structural relations between nucleus and cytoplasm during mitosis inNicotiana tabacum mesophyll. Canad. J. Bot.47, 581–591 + 11 plates.
Euteneuer, U., McIntosh, J. R., 1980: Polarity of midbody and phragmoplast microtubules. J. Cell Biol.87, 509–515.
Furuya, M., 1984: Cell division patterns in multicellular plants. Ann. Rev. Plant Physiol.35, 349–373.
Galatis, B., Apostolakos, P., Katsaros, Chr., 1983: Microtubules and their organizing centres in differentiating guard cells ofAdiantum capillus-veneris. Protoplasma115, 176–192.
Goosen-De Roo, L., Bakhuizen, R., van Spronsen, P. C., Libbenga, K. R., 1984: The presence of extended phragmosomes containing cytoskeletal elements in fusiform cambial cells ofFraxinus excelsior L. Protoplasma122, 145–152.
Gunning, B. E. S., 1982: The cytokinetic apparatus: Its development and spatial regulation. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 229–292. London-New York: Academic Press.
Hardham, A. R., Gunning, B. E. S., 1978: Structure of cortical microtubule arrays in plant cells. J. Cell Biol.77, 14–34.
Hepler, P. K., Jackson, W. T., 1968: Microtubules and early stages of cell-plate formation in the endosperm ofHaemanthus katherinae Baker. J. Cell Biol.38, 437–446.
Klasterska, I., 1976: A new look on the role of the diffuse stage in problems of plant and animal meiosis. Hereditas82, 193–203.
—,Ramel, C., 1979: Prophase of plant meiosis: Sequences and interpretations of stages. Genetica51, 15–20.
Lambert, A.-M., Bajer, A. S., 1972: Dynamics of spindle fibers and microtubules during anaphase and phragmoplast formation. Chromosoma (Berl.)39, 101–144.
Lenoir, M., 1932: Evolution des chromosomes heterotypiques pendant la diacinese chez l'Equisetumpalustre (L.). C. R. Soc. Biol. Paris110, 77–78.
Lloyd, C. W., Barlow, P. W., 1982: The co-ordination of cell division and elongation: The role of the cytoskeleton. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 203–228. London-New York: Academic Press.
Marchant, H. J., Pickett-Heaps, J. D., 1973: Mitosis and cytokinesis inColeochaete scutata. J. Phycol.9, 461–471.
O'Brien, T. P., 1983: The preprophase band of microtubules: does it block cleavage? Cytobios37, 101–105.
Palevitz, B. A., Hepler, P. K., 1974: The control of the plane of division during stomatal differentiation inAllium. I. Spindle reorientation. Chromosoma46, 297–326.
Pickett-Heaps, J. D., 1967: Ultrastructure and differentiation inChara sp. I. Vegetative cells. Aust. J. biol. Sci.20, 539–551.
Schnepf, E., 1984: Preand postmitotic reorientation of microtubule arrays in youngSphagnum leaflets: Transitional stages and initiation sites. Protoplasma120, 100–112.
Wick, S. M., Duniec, J., 1984: Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between preprophase band and the mitotic spindle. Protoplasma122, 45–55.
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Brown, R.C., Lemmon, B.E. A cytoskeletal system predicts division plane in meiosis ofSelaginella . Protoplasma 127, 101–109 (1985). https://doi.org/10.1007/BF01273706
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DOI: https://doi.org/10.1007/BF01273706