Abstract
Acid phosphatase activity was mainly localized in the lysosomes in all the regions of the outer epithelium. The transitional portion of the outer epithelium showed more intense activity than the other regions. During shell regeneration the activity of this portion decreased to a minimum level at 12 hours and was restored to normal at 72 hours. The other regions showed no change of activity during shell regeneration. It is postulated that the acid phosphatase in the transitional protion is responsible for conferring calcifiability to the organic matrix of the shell.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bevelander, G., Nakahara, H.: Correlation of lysosomal activity and ingestion by the mantle epithelium. Biol. Bull.131, 76–82 (1966)
Etherton, J. E., Botham, C. M.: Factors affecting lead capture methods for the fine localization of rat lung acid phosphatase. Histochem. J.2, 507–519 (1970)
Ganagarajah, M., Saleuddin, A. S. M.: Electron histochemistry of the outer mantle epithelium inHelix during shell regeneration. Proc. Malac. Soc. (Lond.)40, 71–77 (1972)
Greenspan, J. S., Blackwood, H. J. J.: Histochemical studies of the chondrocyte function in the cartilage mandibular condyle of the rat. In: Calcified tissue 1965. Proc. 3rd European Symp. on Calcified Tissue (H. Fleish, H. J. J. Blackwood, M. Owen, eds.), p. 40–44. New York: Springer 1966
Kado, Y.: Studies on shell formation in molluscs. J. Sci., Hiroshima Univ., Series B, Div. 119, 163–210 (1960)
Kapur, S. P., Gibson, M. A.: A histological study of the development of the mantle edge and shell inHelisoma duryi eudiscus (Pilsbry). Canad. J. Zool.45, 1169–1181 (1967)
Kapur, S. P., Gibson, M. A.: A histochemical study of the development of the mantle edge and shell in the freshwater gastropod,Helisoma duryi eudiscus (Pilsbry). Canad. J. Zool.46, 489–491 (1968)
Matsuzawa, T., Anderson, H. C.: Phosphatases of epiphyseal cartilage studied by electron microscopic cytochemical methods. J. Histochem. Cytochem.19, 801–808 (1971)
Matukas, V. J., Krikos, G. A.: Evidence for changes in protein polysaccharide associated with the onset of calcification in cartilage. J. Cell Biol.39, 43–48 (1968)
McLean, F. C., Urist, M. R.: In: Bone: Fundamentals of the physiology of skeletal tissue 3rd. ed., p. 25–29. Chicago: The University of Chicago Press 1968
Nakahara, H., Bevelander, G.: Ingestion of particulate matter by the outer surface cells of the mollusc mantle. J. Morph.122, 139–146 (1967)
Saleuddin, A. S. M.: Electron microscopic study of mantle of normal and regeneratingHelix. Canad. J. Zool.48, 409–416 (1970)
Timmermans, L. P. M.: Studies on shell formation in molluscs. Netherlands J. Zool.19, 417–523 (1969)
Tse, V., Saleuddin, A. S. M.: Studies on shell regeneration inHelisoma durgi. Proc. Can. Federa. Biol. Soc.14, 82 (abstract) (1971)
Wong, V., Saleuddin, A. S. M.: Fine structure of normal and regenerated shell ofHelisoma duryi duryi. Canad. J. Zool.50, 1563–1568 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chan, J.F.Y., Saleuddin, A.S.M. Acid phosphatase in the mantle of the shell-regenerating snailHelisoma duryi duryi . Calc. Tis Res. 15, 213–220 (1974). https://doi.org/10.1007/BF02059058
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02059058