Summary
In vivo calcium absorption was studied in normal and rachitic chicks. Cytochalasin B (CB) at a concentration of 25 µg/ml added to the medium inside the duodenal lumen inhibited calcium absorption (20 min) from 82.5±1.9% of calcium absorbed in the controls to 59.2±3% in normal and from 70.0±2.3% to 47.0±2.1% in rachitic chicks. In vitro studies by everted ileal sacs of young rabbits also showed an inhibition of active transport of calcium due to CB. Whereas in the controls the ratio of45Ca concentrations in serosal and mucosal media (60 min) was 7.2±0.32, the ratios were 5.24±0.52; 4.40±0.36; 3.40±0.42; 5.77±0.52; 1.38±0.08; and 1.06±0.02 in the presence of CB at concentrations of 5, 10 and 25 µg/ml; colchicine 10−4M, Na citrate 0.02M, and heat-devitalized conditions, respectively.45Ca concentration in the mucosal scrapings was also affected. It showed an increase from controls (15,101±404 cpm/mg) and correlated with CB concentration: 17,378±489, 19,015±1000, and 20,201±362 at 5, 10, and 25 µg/ml, respectively. Dihydrocytochalasin B also inhibited active calcium transport and caused an increase in45Ca concentration in the mucosal scrapings. Correlated electron microscopic studies showed certain changes in the brush border, especially in some actin microfilaments in the terminal web region. It seems that these morphological alterations may be related to transcytoplasmic movement of calcium.
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References
Lehninger, A.: Mitochondria and calcium ion transport, Biochem. J.119:129–138, 1970
Sampson, H.W., Mathews, J.L., Martin, J.H., Kunin, A.S.: Electron microscopic localization of calcium in the small intestine of normal, rachitic, and vitamin D-treated rats, Calcif. Tissue Res.5:305–316, 1970
Weringer, E.J., Oldham, S.B., Bethune, J.E.: A proposed cellular mechanism for calcium transport in the intestinal epithelial cells, Calcif. Tissue Res.26:71–79, 1978
Jande, S.S., Brewer, L.M.: Effects of vitamin D3 on duodenal absorptive cells of chicks, an electron microscopic study, Z. Anat. Entwickl. Gesch.144:249–265, 1974
DeLuca, H.F.: Vitamin D and calcium transport. Calcium transport and cell function, Ann. N.Y. Acad. Sci.307:356–376, 1978
Wilson, P.W., Lawson, D.E.M.: 1,25-Dihydroxyvitamin D stimulation of specific membrane proteins in chick intestine, Biochim. Biophys. Acta497:805–811, 1977
Wilson, P.W., Lawson, D.E.M.: Incorporation of (3H)leucine into actin-like protein in response to 1,25-dihydroxycholecalciferol in chick intestinal brush borders, Biochem. J.173:627–631, 1978
Wessells, N.K., Spooner, B.S., Ash, J.F., Bradley, M.O., Ludena, M.A., Taylor, E.L., Wrenn, J.T., Yamada, K.M.: Microfilaments in cellular and developmental processes, Science171:135–143, 1971
Spooner, B.S.: Cytochalasin B: towards an understanding of its mode of action, Dev. Biol.35:F13–18, 1973
Fuchs, R., Peterlink, M.: Vitamin D-induced transepithelial phosphate and calcium transport by chick jejunum. Effect of microfilamentous and microtubular inhibitors, FEBS Lett.100:357–359, 1979
Schachter, D., Rosen, S.M.: Active transport of Ca45 by the small intestine and its independence on vitamin D, Am. J. Physiol.196:357–362, 1959
Hurwitz, S., Harrison, H.C., Harrison, H.E.: Effect of vitamin D3 on the in vitro transport of calcium by the chick intestine, J. Nutr.91:319–323, 1967
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixation of high osmolarity for use in electron microscopy, J. Cell Biol.27:137A, 1965
Maupin-Szamier, P., Pollard, T.D.: Actin filament destruction by osmium tetroxide, J. Cell Biol.77:837–852, 1978
Hull, B.E., Staehelin, L.A.: The terminal web: a re-evaluation of its structure and function, J. Cell Biol.81:67–82, 1979
Mizel, S.B., Wilson, L.: Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B, J. Biol. Chem.247:4102–4105, 1972
Kletzien, R.F., Perdue, J.F., Springer, A.: Cytochalasin A and B: inhibition of sugar uptake in cultured cells, J. Biol. Chem.247:2964–2966, 1972
Atlas, S.J., Lin, S.: Dihydrocytochalasin B. Biological effects and binding to 3T3 cells, J. Cell Biol.76:360–370, 1978
Burgess, D.R., Grey, R.D.: Alteration in morphology of developing microvilli elicited by cytochalasin B, J. Cell Biol.62:566–574, 1974
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Jande, S.S., Liskova-Kiar, M. Effects of cytochalasin B and dihydrocytochalasin B on calcium transport by intestinal absorptive cells. Calcif Tissue Int 33, 143–151 (1981). https://doi.org/10.1007/BF02409427
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DOI: https://doi.org/10.1007/BF02409427