Summary
Adventitious bud formation was induced on detached cotyledons and on cotyledons attached to excised embryos of Pinus caribaea and Pseudotsuga menziesii. The embryonic tissue was exposed to the cytokinin 6-benzyl amino purine contained within an agar medium. This exposure resulted in the formation of a meristematic zone, involving both epidermal and sub-epidermal cells, and then nodules on the tissue surface. Some of these nodules were induced to differentiate into bud primordia, and thence shoots, following exposure to a combination of auxin (IAA) and cytokinin. Shoots were produced over the entire surface of detached cotyledons of Ps. menziesii but predominantly on the adaxial surfaces of detached cotyledons of Pi. caribaea and the tips and adaxial surfaces of the cotyledons on the entire embryos of both species. Thus, inter-specific differences in the distribution of competent areas for adventitious bud production were detected in embryos.
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Skidmore, D.I. Adventitious bud development in embryonic tissue of Pinus caribaea and Pseudotsuga menziesii . Trees 2, 32–38 (1988). https://doi.org/10.1007/BF00196977
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DOI: https://doi.org/10.1007/BF00196977