Abstract
Despite the importance of the vascular cambial differentiation, little is known about its regulation. In order to address this problem we attempted to biochemically characterize differentiating xylem and phloem elements during the early stages of development. By applying techniques of quantitative histochemistry we show that the total pool size of pyridine nucleotides is similar in the phloem (PD) and xylem (XD) oriented derivatives of the cambial zone of trees ofRobinia pseudoacacia L. Within the PD zone, the amount of NAD + NADH exceeded that of NADP + NADPH [around 600 versus 200 pmol (mg dry weight)-1], possibly indicative of a preponderance of catabolic pathways (ratio of NADH∶NAD about 1). In contrast, the NADP(H) system dominated in the XD zone. This coincided with a high activity of NAD kinase. In addition, the extractable activities of the key enzymes of the oxidative pentose phosphate pathway, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, were greatly increased. At a ratio of NADPH∶NADP of approximately 1, this could be indicative of increased rates of reductive biosyntheses, and could thus well be involved in early steps of the formation of phenols and lignin monomers. Taken together, this first approach clearly shows that phloem-oriented and xylem-oriented cambial descendents exhibit distinct differences in their biochemical patterns even in early stages of differentiation.
Similar content being viewed by others
References
Ashihara H, Komamine A (1974) Regulation of the activities of some enzymes of the pentose phosphate pathway inPhaseolus mungo. Z Pflanzenphysiol 36: 52–59
Barnett JR (1992) Reactivation of the cambium inAesculus hippocastanum L.: a transmission electron microscopic study. Ann Bot 70: 169–177
Bonzon M, Simon P, Greppin H, Wagner E (1983) Pyridine nucleotides and redox-charge evolution during the induction of flowering in spinach leaves. Planta 159: 254–260
Brinkman FG, Donkers CK, Smelt JJ, van der Plas LHW (1982) Respiration rate and redox state of NAD during wound induced differentiation of potato tuber tissue (Solanum tuberosum L.). Plant Sci Lett 27: 29–36
Brown DCW, Thorpe TA (1980) Adenosine phosphate and nicotinamide adenine dinucleotide pool sizes during shoot initiation in tobacco callus. Plant Physiol 65: 587–590
Catesson AM (1989) Specific characters of vessel primary walls during the early stages of wood differentiation. Biol Cell 67: 221–226
Catesson AM (1994) Cambial ultrastructure and biochemistry: changes in relation to vascular tissue differentiation and the seasonal cycle. Int J Plant Sci 155:251–261
Egger B, Hampp R (1993) Invertase, sucrose synthase and sucrose phosphate synthase in lyophilized spruce needles; microplate reader assays. Trees 7: 98–103
Gabriel MK, McGuinness ET (1984) NAD+ kinase: molecular weight determination by low-angle laser-light scattering. FEBS Lett 175: 419–421
Gander JE (1982) 2. Polyhydroxy acids: relation to hexose phosphate metabolism. In: Loewus FA, Tanner W (eds) Plant carbohydrates I. Intracellular carbohydrates. Encyclopaedia of plant physiology, new series, vol 13 A. Springer, Berlin Heidelberg New York, pp77–102
Guttenberger M, Neuhoff V, Hampp R (1991) A dot-blot assay for quantitation of nanogram amounts of protein in the presence of carrier ampholytes and other possible interfering substances. Anal Biochem 196:99–103
Guttenberger M, Schaeffer C, Hampp R (1993) Kinetic and electrophoretic characterization of NADP dependent dehydrogenases from root tissues of Norway spruce [Picea abies (L.) Karst.] employing a rapid one-step extraction procedure. Trees 8: 191–197
Hampp R, Goller M, Füllgraf H (1984) Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymatic cycling. Plant Physiol 75: 1017–1021
Hampp R, Rieger A, Outlaw WH Jr (1990) Microdissection and biochemical analysis of plant tissues. In: Linskens HF, Jackson JF (eds) Physical methods in plant sciences. Springer, Berlin Heidelberg New York, pp 124–147
Heber UW, Santarius KA (1965) Compartmentation and reduction of pyridine nucleotides in relation to photosynthesis. Biochim Biophys Acta 109: 390–408
Kato T, Berger SJ, Carter JA, Lowry OH (1973) An enzymatic cycling method for nicotinamide-adenine dinucleotide with malic and alcohol dehydrogenases. Anal Biochem 53: 86–97
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Lieckfeldt E, Jung SJ, Peine G, Hoffmann P (1987) Pyridine nucleotides in selected plant species: ecological and evolutionary aspects. Biochem Physiol Pflanzen 182: 393–405
Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic Press, New York
Meyer R, Wagner KG (1986) Nucleotide pools in leaf and root tissue of tobacco plants: influence of leaf senescence. Physiol Plant 67: 666–672
Miller AR, Crawford DL, Roberts LW (1985) Lignification and xylogenesis inLactuca pith explants cultured in vitro in the presence of auxin and cytokinin: a role of endogenous ethylene. J Exp Bot 36: 110–118
Northcote DH (1995) Aspects of vascular tissue differentiation in plants: parameters that may be used to monitor the process. Int J Plant Sci 156: 245–256
Oka KI, Takahashi T, Hori SH (1981) Differential effects of the NAPDH/NADP ratio on the activities of hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase. Biochim Biophys Acta 662: 318–325
Pryke JA, apRees T (1977) The pentose phosphate pathway as a source of NADPH for lignin synthesis. Phytochem 16: 557–560
Pryke JA, apRees T (1976) Activity of pentose phosphate pathway during lignification. Planta 132: 279–284
Sagisaka S, Asada M (1981) Coordinate and noncoordinate changes in enzyme activities in pentose phosphate cycle in poplar: a control of enzyme activities in differentiated xylem. Plant Cell Physiol 22: 1459–1468
Sauer A, Robinson DG (1985) Calmodulin dependent NAD-kinase is associated with both the outer and inner mitochondrial membranes in maize roots. Planta 166: 227–233
Sies H (1982) Nicotinamide nucleotide compartmentation. In: Sies H (ed) Metabolic compartmentation. Academic Press, London, pp 205–231
Simon P, Dieter P, Bonzon M, Greppin H, Marmé D (1982) Calmodulin-dependent and independent NAD kinase activities from cytoplasmic and chloroplastic fractions of spinach (Spinacia oleracea L.). Plant Cell Rep 1: 119–122
Simon P, Bonzon M, Greppin H, Marmé D (1984) Subchloroplastic localization of NAD kinase activity: evidence for a Ca2+, calmodulin-dependent activity at the envelope and for a Ca2+, calmodulin-independent activity in the stroma of pea chloroplasts. FEBS Lett 167: 332–338
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Magel, E., Bleuel, H., Hampp, R. et al. Pyridine nucleotide levels and activities of dehydrogenases in cambial derivatives ofRobinia pseudoacacia L.. Trees 10, 325–330 (1996). https://doi.org/10.1007/BF02340779
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02340779