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Immunogold labelling of estradiol receptor in MCF7 cells

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Abstract

The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an estradiol-induced translocation of this ligand-dependent transcription regulator.

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  1. Max-Planck-Institut für Experimentelle Endokrinologie, Postfach 610309, D-30603, Hannover, Germany

    Walter D. Sierralta, Ingrid Bönig & Hubert H. Thole

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  1. Walter D. Sierralta
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  2. Ingrid Bönig
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  3. Hubert H. Thole
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Sierralta, W.D., Bönig, I. & Thole, H.H. Immunogold labelling of estradiol receptor in MCF7 cells. Cell Tissue Res 279, 445–452 (1995). https://doi.org/10.1007/BF00318156

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  • DOI: https://doi.org/10.1007/BF00318156

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