Skip to main content
Log in

Immunocytochemical study of the lung of domestic fowl and pigeon: endocrine cells and nerves

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

the presence of endocrine cells and nerves in the lung of 2 avian species (Gallus gallus and Columba livia domestica) has been studied by peroxidase-antiper-oxidase (PAP) and avidin-biotin complex (ABC) immunocytochemical methods at the light-microscopic level. Two immunoreactive cell-types have been identified in the epithelium of the primary and secondary bronchi of chick lung: serotonin- and bombesin-immunoreactive cells; and 3 cell-types, namely, serotonin-, bombesin- and CGRP-(calcitonin gene related peptide) immunoreactive cells, have been located in the bronchial epithelium of pigeon lung. Co-localization of 2 different immunoreactivities within the same cell has not been detected. VIP-immunoreactive nerves have been observed in different locations in chick lung.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barnes PJ (1987) Regulatory peptides in the respiratory system. Experientia 43:832–839

    Google Scholar 

  • Bensch KG, Gordon GB Miller LR (1965) Studies on the bronchial counterpart of the Kultschitzky (argentaffin) cell and innervation of bronchial glands. J Ultrastruc Res 12:668–686

    Google Scholar 

  • Brain SD, Willians TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene-related peptide is a potent vasodilator. Nature 313:54–56

    Google Scholar 

  • Burger RE, Osborne JL, Banzett RB (1974) Intrapulmonary chemoreceptors in Gallus domesticus. Adequate stimulus and functional localization. Respir Physiol 22:87–97

    Google Scholar 

  • Capella C, Hage E, Solcia E, Usellini L (1978) Ultrastructural similarity of endocrine-like cells of the human lung and some related cells of the gut. Cell Tissue Res 186:25–37

    Google Scholar 

  • Cook RD, King AS (1969) A neurite-receptor complex in the avian lung: electron microscopical observations. Experientia 25:1162–1164

    Google Scholar 

  • Cutz E (1982) neuroendocrine cell of the lung — an overview of morphologic characteristics and development. Exp Lung Res 3:185–208

    Google Scholar 

  • Cutz E, Conen PE, (1972) Endocrine-like cells in human fetal lungs. An electron microscopic study. Anat Rec 173:115–122

    Google Scholar 

  • Cutz E, Gillan JE, Track NS (1984) Pulmonary endocrine cells in the developing human lung and during neonatal adaptation. In: Becker KL, Gazdar AF (eds) The endocrine lung in health and disease. Saunders, Philadelphia, pp 210–231

    Google Scholar 

  • Cutz E, Goniakowska-Witalinska L, Chan W (1986) An immunohistochemical study of regulatory peptides in lungs of amphibians. Cell Tissue Res 244:227–233

    Google Scholar 

  • Dayer AM, De Mey J, Will JA (1985) Localization of somatostatin-, bombesin, and serotonin-like immunoreactivity in the lung of the fetal rhesus monkey. Cell Tissue Res 239:621–625

    Google Scholar 

  • Dey RD (1986) Colocalization of VIP and SP in nerves innervating bronchial smooth muscle and glands. Anat Rec 214:32A

    Google Scholar 

  • Dey RD, Shanmon WA, Said SI (1981) Localization of VIP-immunoreactive nerves in airways and pulmonary vessels of dogs, cats and human subjects. Cell Tissue Res 220:231–238

    Google Scholar 

  • Eaton JA, Fedde MR (1980) Demonstrating biogenic amine containing cells in the avian lung by the paraformaldehyde perfusion technique. Poultry Sci 59:921–923

    Google Scholar 

  • Ericson LE, Håkanson R, Larson B, Owman C, Sundler F (1972) Fluorescence and electron microscopy of amine-storing enterochromaffin-like cells in tracheal epithelium of mouse. Z Zellforsch 124:532–545

    Google Scholar 

  • Ghatei MA, Sheppard M, O'Shaunessy DJ, Adrian TE, MacGregor JM, Polak JM, Bloom SR (1982) Regulatory peptides in the mammalian respiratory tract. Endocrinology 111:1248–1254

    Google Scholar 

  • Ghatei MA, Sheppard M, Henzen-Logman S, Blank MA, Polak JM, Bloom SR (1983) Bombesin and vasoactive intestinal polypeptide in the developing lung: marked changes in acute respiratory distress syndrome. J Clin Endocr 57:1226–1232

    Google Scholar 

  • Goniakowska-Witalinska L, Cutz E (1990) Ultrastructure of neuroendocrine cells in the lungs of three anuran species. J Morphol 203:1–9

    Google Scholar 

  • Hage E (1973) Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses. Cell Tissue Res 141:401–412

    Google Scholar 

  • Hage E (1980) Light and electron microscopic characteristic of the various lung endocrine cell types. Invest Cell Pathol 3:345–351

    Google Scholar 

  • Holmgren S, Axelsson M, Jensen J, Aldman G, Sundell K, Jonsson AC (1989) Bombesin-like immunoreactivity and the effect of bombesin in the gut, circulatory system and lung of the caiman, Caiman crocodylus crocodylus and the crocodyle, Crocodylus porosus. Exp Biol 48:261

    Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    Google Scholar 

  • Hung K, Hertweck MS, Hardy JD, Loosli CG (1973) Ultrastructure of nerves and associated cells in bronchiolar epithelium of the mouse lung. J Ultrastruc Res 43:426–437

    Google Scholar 

  • Impicciatore M, Bertaccini G (1973) The bronchoconstrictor action of the tetradecapeptide bombesin in the guinea pig. J Pharm Pharmacol 25:872–875

    Google Scholar 

  • Johnson MD, Gray ME, Stahlman MT (1988) Calcitonin generelated peptide in human fetal lung and in neonatal lung disease. J Histochem Cytochem 36:199–204

    Google Scholar 

  • King AS, McLelland J, Cook RD, King DZ, Walsh C (1974) The ultrastructure of afferent nerve endings in the avian lung. Respir Physiol 22:21–40

    Google Scholar 

  • Laitinen A, Partanen M, Heruonen A, Peto-Huikko M, Laitinen LA (1985) VIP-like immunoreactive nerves in human respiratory tract. Light and electron microscopic study. Histochemistry 82:313–319

    Google Scholar 

  • Lane BP, Europa DL (1965) differential staining of ultrathin sections of Epon embedded tissues for light microscopy. J Histochem Cytochem 13:579–582

    Google Scholar 

  • Lauweryns JM, Cokelaere M (1973) Hypoxia-sensitive neuro-epithelial bodies. Intrapulmonary secretory neuroreceptors, modulated by the CNS. Z Zellforsch 145:521–540

    Google Scholar 

  • Lauweryns JM, Van Ranst L (1987) Calcitonin gene-related peptide immunoreactivity in rat lung: light and electron microscopic study Thorax 42:183–189

    Google Scholar 

  • Lauweryns JM, Cokelaere M, Theuninck P (1972) neuroepithelial bodies in the respiratory mucosa of various mammals. Z Zellforsch 135:569–592

    Google Scholar 

  • Lauweryns JM, Bock V de, Verhofstad AAJ, Steinbusch HWM (1982) Immunohistochemical localization of serotonin in intrapuimonary neuroepithelial bodies. Cell Tissue Res 226:215–223

    Google Scholar 

  • Lauweryns JM, Van Ranst L, Verhofstad AAJ (1986) Ultrastructural localization of serotonin in the intrapulmonary neuroepithelial bodies of neonatal rabbits by use of immuno-electron microscopy. Cell Tissue Res 243:455–459

    Google Scholar 

  • López J, Diaz de Rada O, Sesma P, Vázquez JJ (1983a) Silver methods applied to semithin sections to identify peptide-producing endocrine cells. Anat Rec 205:465–470

    Google Scholar 

  • López J, Sesma P, Vázquez JJ (1983b) Estudio histológico del epitelio del mesobronquio y bronquios secundarios del pulmón de Gallus gallus. Morf Norm Patol 7:215–229

    Google Scholar 

  • Mayor HD, Hampton JC, Rosario BA (1961) A simple method for removing the resin from epoxy-embedded tissue. J Biophys Biochem Cytol 9:909

    Google Scholar 

  • McLelland J, Molony V (1983) Respiration. In: Freeman BM (ed) Physiology and biochemistry of the domestic fowl, vol 4. Academic Press, London, New York, pp 63–89

    Google Scholar 

  • McLelland J, Macfarlane CJ (1986) Solitary granular endocrine cells and neuroepithelial bodies in the lungs of the ringed turtle dove (Streptopelia risoria). J Anat 147:83–93

    Google Scholar 

  • Palmer JBD, Cuss FMC, Ghatei MA, Springall DR, Cadieux A, Bloom SR, Polak JM, Barnes PJ (1985) Calcitonin gene-related peptide is localised to human airways nerves and potently constricts human airway smooth muscle. Thorax 40:713

    Google Scholar 

  • Pastor LM, Ballesta J, Pérez-Tomás R, Marin JA, Hernández F, Madrid JF (1987) Immunocytochemical localization of serotonin in the reptilian lung. Cell Tissue Res 248: 713–715

    Google Scholar 

  • Pastor LM, Ballesta J, Castells MT, Pérez-Tomás R, Marin JA, Madrid JF (1989) A microscopic study of the lung of Testudo graeca (Chelonia). J Anat 164:19–39

    Google Scholar 

  • Polak JM, Bloom SR (1985) Occurrence and distribution of regulatory peptides in the respiratory tract. Rec Res Cancer Res 99:1–16

    Google Scholar 

  • Polak JM, Bloom SR, (1986), Regulatory peptides of the gastrointestinal and respiratory tracts. Archs Int Pharmacodyn 280 [Suppl]:16–49

    Google Scholar 

  • Priestley JV (1984) Pre-embedding ultrastructural immunocytochemistry: Immunoenzyme techniques. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy. Elsevier, New York, pp 37–52

    Google Scholar 

  • Ravazzola M, Orci L, Girgis SI, Galan Galan F, McIntyre I (1981) The lung is the major organ source of calcitonin in the lizard. Cell Biol Int Rep 5:937–944

    Google Scholar 

  • Rogers DC, Haller CJ (1978) Innervation and cytochemistry of the neuro-epithelial bodies in the ciliated epithelium of the toad lung (Bufo marinus). Cell Tissue Res 195:395–410

    Google Scholar 

  • Scheuermann DW, De Groodt-Lasseel MHA, Stilman C, Meisters ML (1983) A correlative light-, fluorescence- and electron-microscopic study of neuroepithelial bodies in the lung of the red-eared turtle, Pseudemys scripta elegans. Cell Tissue Res 234:249–269

    Google Scholar 

  • Scheuermann DW, Timmermans JP, Adriaensen D, De Groodt-Lasseel MHA (1987) Immunoreactivity for calcitonin generelated peptide in neuroepithelial bodies of newborn cat. Cell Tissue Res 249:337–340

    Google Scholar 

  • Sorokin SP, Hoyt RF (1978) PAS-lead hematoxylin as a stain for small granule endocrine cell populations in the lung, other pharyngeal derivatives and the gut. Anat Rec 192:245–260

    Google Scholar 

  • Sorokin SP, Hoyt RF (1989) Neuroepithelial bodies and small-granule cells. In: Massaro D (ed) Lung Cell Biology. Lung biology in health and disease, vol 41. Dekker, New York, pp 191–344

    Google Scholar 

  • Stahlman MT, Gray ME (1984) Ontogeny of neuroendocrine cells in human fetal lung. I. An electron microscopic study. Lab Invest 51:449–463

    Google Scholar 

  • Stefanini M, Martino C de, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173–174

    Google Scholar 

  • Sternberger LA (1979) Immunochemistry. 2nd edn. Wiley, New York

    Google Scholar 

  • Walsh C, McLelland J (1974) Granular “endocrine” cells in avian respiratory epithelia. Cell Tissue Res 153:269–276

    Google Scholar 

  • Wasano K, Yamamoto T (1979) APUD-type recepto-secretory cells in the chicken lung. Cell Tissue Res 201:197–205

    Google Scholar 

  • Wasano K, Yamamoto T (1981) A scanning and transmission electron-microscopic study on neuro-epithelial bodies in the neonatal mouse lung. Cell Tissue Res 216:481–490

    Google Scholar 

  • Wharton J, Polak JM, Bloom SR, Ghatei MA, Solcia E, Brown MR, Pearse AGE (1978) Bombesin-like immunoreactivity in the lung. Nature 273:769–770

    Google Scholar 

  • Zaccone G, Tagliaferro L, Goniakowska-Witalinska L, Fasulo S, Ainis L, Mauceri A (1989) Serotonin-like immunoreactive cells in the pulmonary epithelium of ancient fish species. Histochemistry 92:61–63

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

López, J., Barrenechea, M.A., Burrell, M.A. et al. Immunocytochemical study of the lung of domestic fowl and pigeon: endocrine cells and nerves. Cell Tissue Res 273, 89–95 (1993). https://doi.org/10.1007/BF00304615

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00304615

Key words

Navigation