Summary
The immunoreactive CRF-neurons of the rat hypothalamus have been examined immunohistochemically employing anti-rat CRF serum. These neurons are confined to the paraventricular nucleus, dorsomedial-lateral hypothalamic area, and suprachiasmatic nucleus, and are, respectively, also immunoreactive to anti-Met-enk, -alpha-MSH, and -VIP sera. Intraventricular administration of colchicine (50 μg/5 μl/rat) induces a dramatic enhancement of the immunostainability of the cell somata, and also accelerates the development of immunoreactivity of other stored peptides, especially in the paraventricular nucleus.
The CRF-neurons respond to adrenalectomy by showing increased immunoreactivity and an increase in the number of cell bodies; in the dorsomedial-lateral area and suprachiasmatic nucleus, there is also an enhanced immunoreactivity for alpha-MSH and VIP, respectively. CRF-cells in the paraventricular nucleus become markedly hypertrophied, but do not show any enhanced immunoreactivity for Met-enk. Since the axons of the paraventricular neurons run to the median eminence, it is probable that they are involved with the endocrine control of hypophysial ACTH release. It is concluded that the CRF-containing neurons in rat hypothalamus consist of three types which are functionally and morphologically different.
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References
Bloom FE, Battenberg ELF, Rivier J, Vale W (1982) Corticotropin releasing factor (CRF): immunoreactive neurones and fibers in rat hypothalamus. Regul Pept 4:43–48
Bugnon C, Fellmann D, Gouget A, Cardot J (1982) Corticoliberin in rat brain: immunocytochemical identification and localization of a novel neuroglandular system. Neurosci Lett 30:25–30
Card JP, Brecha N, Karten HJ, Moore RY (1981) Immunocytochemical localization of vasoactive intestinal polypeptide-containing cells and processes in the suprachiasmatic nucleus of the rat: light and electron microscopic analysis. J Neurosci 1:1289–1303
Cummings S, Elde R, Ells J, Lindall A (1983) Corticotropin-releasing factor immunoreactivity is widely distributed within the central nervous system of the rat: an immunohistochemical study. J Neurosci 3:1355–1368
Daikoku S, Chikamori M, Adachi T, Okamura Y, Nishiyama T, Tsuruo Y (1983) Ontogenesis of hypothalamic immunoreactive ACTH cells in vivo and in vitro: role of Rathke's pouch. Dev Biol 97:81–88
Daikoku S, Okamura Y, Kawano H, Tsuruo Y, Maegawa M, Shibasaki T (1984) Immunohistochemical study on the development of CRF-containing neurons in the hypothalamus of the rat. Cell Tissue Res 238:539–544
Hökfelt T, Lundberg JM, Schultzberg M, Johansson O, Ljungdahl Å, Rehfeld J (1980) Coexistence of peptides and putative transmitters in neurons. In: Costa E, Trabucchi M (eds) Neural peptides and neuronal communication. Raven Press, New York, pp 1–23
Hökfelt T, Fahrenkrug J, Tatemoto K, Mutt V, Werner S, Hulting A-L, Terenius L, Chang KJ (1983) The PHI(PHI-27)/corticotropin-releasing factor/enkephalin immunoreactive hypothalamic neuron: possible morphological basis for integrated control of prolactin, Corticotropin, and growth hormone secretion. Proc Natl Acad Sci USA 80:895–898
Jegou S, Tonon MC, Guy J, Vaudry H, Pelletier G (1983) Biological and immunological characterization of α-melanocyte-stimulating hormone (α-MSH) in two neuronal systems of the rat brain. Brain Res 260:91–98
Kawano H, Daikoku S, Saito S (1982) Immunohistochemical studies of intrahypothalamic somatostatin-containing neurons in rat. Brain Res 242:227–232
Kiss JZ, Mezey E, Skirboll L (1984) Corticotropin-releasing factor-immunoreactive neurons of the paraventricular nucleus become vasopressin positive after adrenalectomy. Proc Natl Acad Sci USA 81:1854–1858
Kobayashi S, Uchida T, Ohashi T, Fujita T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N (1983) Met-enkephalin-Arg-Gly-Leu-like immunoreactivity in adrenal chromaffin cells and carotid body chief cells of the dog and monkey. Biomed Res 4:201–210
Makara GB, Stark E, Kárteszi M, Palkovitz M, Rappay GY (1981) Effects of paraventricular lesions on stimulated ACTH release and CRF in stalk-median eminence of the rat. Am J Physiol 240:E441-E446
Merchenthaler I, Vigh S, Petrusz P, Schally AV (1983) The paraventriculo-infundibular Corticotropin releasing factor (CRF) pathway as revealed by immunocytochemistry in long-term hypophysectomized or adrenalectomized rats. Regul Pept 5:295–305
Miller RJ, Pickel VM (1980) The distribution and functions of the enkephalins. J Histochem Cytochem 28:903–917
O'Donohue TL, Dorsa DM (1982) The opiomelanotropinergic neuronal and endocrine systems. Peptides 3:353–395
Olschowka JA, O'Donohue TL, Mueller GP, Jacobowitz DM (1982) The distribution of corticotropin releasing factor-like immunoreactive neurons in rat brain. Peptides 3:995–1015
Paull WK, Gibbs FP (1983) The corticotropin releasing factor (CRF) neurosecretory system in intact, adrenalectomized, and adrenalectomized-dexamethasone treated rats. Histochemistry 78:303–316
Paull WK, Schöler J, Arimura A, Meyers CA, Chang JK, Chang D, Shimizu M (1982) Immunocytochemical localization of CRF in the ovine hypothalamus. Peptides 1:183–191
Proulx-Ferland L, Labrie F, Dumont D, Côté J (1982) Corticotropin-releasing factor stimulates secretion of melanocyte-stimulating hormone from the rat pituitary. Science 217:62–63
Rivier C, Brownstein M, Spiess J, Rivier J, Vale W (1982) In vivo corticotropin-releasing factor-induced secretion of adrenocorticotropin, β-endorphin, and corticosterone. Endocrinology 110:272–278
Sawchenko PE, Swanson EW, Vale WW (1984) Co-expression of corticotropin-releasing factor and vasopressin immunoreactivity in parvocellular neurosecretory neurons of the adrenalectomized rat. Proc Natl Acad Sci USA 81:1883–1887
Sims KB, Hoffman DL, Said SI, Zimmerman EA (1980) Vasoactive intestinal polypeptide (VIP) in mouse and rat brain: an immunocytochemical study. Brain Res 186:165–183
Swanson EW, Sawchenko PE, Rivier J, Vale WW (1983) Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study. Neuroendocrinology 36:165–186
Tatemoto K, Mutt V (1981) Isolation and characterization of the intestinal peptide porcine PHI(PHI-27), a new member of the glucagon-secretin family. Proc Natl Acad Sci USA 78:6603–6607
Tramu G, Croix C, Pillez A (1983) Ability of the CRF immunoreactive neurons of the paraventricular nucleus to produce a vasopressin-like material. Neuroendocrinology 37:467–469
Tsuruo Y, Kawano H, Nishiyama T, Hisano S, Daikoku S (1983) Substance P-like immunoreactive neurons in the tuberoinfundibular area of rat hypothalamus. Light and electron microscopy. Brain Res 289:1–9
Turkelson CM, Arimura A, Culler MD, Fishback JB, Groot K, Kanda M, Euciano M, Thomas CR, Chang D, Chang JK, Shimizu M (1981) In vivo and in vitro release of ACTH by synthetic CRF. Peptides 2:425–429
Turner JD, Keith AB, Smith AI, McDermott JR, Biggins JA, Edwardson JA (1983) Studies on the characterisation of α-MSHlike immunoreactivity in rat hypothalamus. Regul Pept 5:283–293
Vacca LL, Abrahams SJ, Naftchi NE (1980) A modified peroxidase-antiperoxidase procedure for improved localization of tissue antigens: localization of substance P in rat spinal cord. J Histochem Cytochem 28:297–307
Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213:1394–1397
Yanaihara N, Sakagami M, Sato H, Yamamoto K, Hashimoto T, Yanaihara C, Ito Z, Yamaguchi K, Abe K (1977) Immunological aspects of secretin, substance P, and VIP. Gastroenterology 72:803–810
Yanaihara N, Yanaihara C, Nokihara K, Iguchi K, Fukata S, Tanaka M, Yamamoto Y, Mochizuki T (1984) Immunochemical study on PHI/PHM with use of synthetic peptides. Peptides 5:247–254
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Daikoku, S., Okamura, Y., Kawano, H. et al. CRF-containing neurons of the rat hypothalamus. Cell Tissue Res. 240, 575–584 (1985). https://doi.org/10.1007/BF00216347
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DOI: https://doi.org/10.1007/BF00216347