Summary
The ultrastructural changes in the adrenal zona glomerulosa and renal juxtaglomerular apparatus have been examined during normal pregnancy in sheep. As pregnancy progressed, increasing numbers of cells in the adrenal zona glomerulosa displayed mitochondria with straight tubular “rod-like” structures replacing their normal lamelliform cristae; groups of cells showing these mitochondrial changes were predominantly located in the middle and superficial regions of the zona glomerulosa, but at all stages remained interspersed with cells with apparently normal mitochondria. In the same animals, the renal juxtaglomerular index was raised, reflecting an increase in renin storage, and juxtaglomerular myoepithelioid cells showed increased numbers of cytoplasmic granules, but no apparent increase in granular endoplasmic reticulum and Golgi profiles; there were no distinguishing morphological changes in juxtaglomerular peripolar cells.
These findings provide morphologic evidence of stimulation of the adrenal zona glomerulosa in association with increased juxtaglomerular renin storage during pregnancy. The mitochondrial changes observed in an increasing proportion of cells in the zona glomerulosa closely resemble those seen in sodium-depleted animals, and may reflect the altered steroidogenic capacity of the adrenal gland in pregnant sheep. The finding of groups of cells displaying altered mitochondria lying next to cells with normal mitochondria suggests the presence of cells with different sensitivities to stimuli for aldosterone production or may indicate the presence of different cell types in the zona glomerulosa responding to different stimuli.
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References
Bowie DJ (1936) Method for staining pepsinogen granules in gastric glands. Anat Rec 64:357–367
Broughton-Pipkin F, Lumbers ER, Mott JC (1974) Factors influencing plasma renin and angiotensin II in the conscious pregnant ewe and its foetus. J Physiol (Lond) 243:619–636
Cook WF (1971) Cellular localisation of renin. In: Fischer JW (ed) Kidney hormones. Academic Press, London, pp 117–128
Davis JO (1967) The regulation of aldosterone secretion. In: Eisenstein AB (ed) The adrenal cortex. Little, Brown and Co, Boston, pp 203–247
Fleischman AR, Oakes, GK, Epstein MF, Catt KJ, Chez RA (1975) Plasma renin activity during ovine pregnancy. Am J Physiol 228:901–904
Glickman JA, Patrick JE, Challis JRG (1980) Increase in the response to adrenocorticotrophin of isolated maternal adrenal cells from sheep in late pregnancy. J Endocrinol 86:93–100
Hartroft PM (1968) The juxtaglomerular complex as an endocrine gland. In: Bloodworth JMB (ed) Endocrine pathology. Williams and Wilkins, Baltimore, pp 641–677
Hill PA, Coghlan JP, Scoggins BA, Ryan GB (1981) Juxtaglomerular peripolar cell secretory activity during sodium depletion and in toxemia of pregnancy in sheep (abst). VIIIth Int Cong Nephrol, Athens, p 4
Hill PA, Coghlan JP, Butkus A, Ryan GB (1983) Structural and functional studies of the adrenal zona glomerulosa in sodium-depleted and sodium-loaded sheep. Cell Tissue Res (accepted for publication)
Kaufman CF, Bergman EN (1978) Renal function studies in normal and toxaemic pregnant sheep. Cornell Vet 68:124–137
Lendrum AC, Fraser DS, Slidders W, Henderson R (1962) Studies on the character and staining of fibrin. J Clin Pathol 15:401–413
Mrsević D, Lacković V, Mujović S, Stefanović S (1980) Morphological aspect of the juxtaglomerular apparatus and adrenal zona glomerulosa during pregnancy in rabbits. Acta Anat 107:418–423
Munger BL, Roth SI (1963) The cytology of the normal parathyroid glands of man and Virginia deer. A light and electron microscopic study with morphologic evidence of secretory activity. J Cell Biol 16:379–400
Nolten WE, Ehrlich EN (1980) Sodium and mineralocorticoids in normal pregnancy. Kidney Int 18:162–172
Parks HF (1962) Morphological study of extrusion of secretory materials by the parotid glands of mouse and rat. J Ultrastruct Res 6:449–465
Peter S, Lazar J, Gross F, Forssmann WG (1974) Studies on the juxtaglomerular apparatus. II. Quantitative morphology after adrenalectomy. Cell Tissue Res 151:457–469
Ryan GB, Coghlan JP, Scoggins BA (1979) The granulated peripolar epithelial cell: a potential secretory component of the renal juxtaglomerular complex. Nature 277:655–656
Smiciklas HA, Pike RL, Schraer H (1971) Ultrastructure of adrenal glands in sodium-deficient pregnant rats. J Nutr 101:1045–1056
Wardlaw JM, Pike RL (1963) Some effects of high and low sodium intake during pregnancy in the rat. IV. Granulation of renal juxtaglomerular cells and zona glomerulosa width. J Nutr 80:355–364
Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26:235–302
Weinberger MH, Kramer NJ, Petersen LP, Cleary RE, Youn PC (1976) Sequential changes in the renin-angiotensin-aldosterone systems and plasma progesterone concentration in normal and abnormal human pregnancy. In: Lindheimer MD, Katz AI, Zuspan FP (eds) Hypertension in pregnancy. John Wiley, New York, pp 263–270
Weir RJ, Brown JJ, Fraser R, Lever AF, Logan RW, McIlwaine GM, Morton JJ, Robertson JIS, Tree M (1975) Relationship between plasma renin, renin-substrate, angiotensin II, aldosterone and electrolytes in normal pregnancy. J Clin Endocrinol Metab 40:108–115
Wintour EM, Blair-West JR, Brown EH, Coghlan JP, Denton DA, Nelson J, Oddie CJ, Scoggins BA, Whipp GT, Wright RD (1976) The effect of pregnancy on mineraloand gluco-corticoid secretion in the sheep. Clin Exp Pharmacol Physiol 3:331–342
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Hill, P.A., Coghlan, J.P., McDougall, J.G. et al. Structural studies of the adrenal zona glomerulosa and renal juxtaglomerular apparatus in pregnant sheep. Cell Tissue Res. 232, 177–187 (1983). https://doi.org/10.1007/BF00222382
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DOI: https://doi.org/10.1007/BF00222382