Summary
The existence of cholecystokinin in the posterior hypophysis and its hypothalamic origin have been unequivocally demonstrated. Immunocytochemical evidence is presented for the coexistence of gastrin-cholecystokinin and oxytocin-neurophysin I immunoreactivities in some magnocellular neurons of the supraoptic and paraventricular nuclei both in rat and bovine hypothalamus.
This is a preview of subscription content, log in via an institution to check access.
References
Beinfeld MC, Meyer DK, Brownstein MJ (1980) Cholecystokinin octapeptide in rat hypothalamoneurohypophyseal system. Nature 288:376–378
Buffa R, Crivelli O, Fiocca R, Fontana P, Solcia E (1979) Complement-mediated unspecific binding of immunoglobulins to some endocrine cells. Histochemistry 63:15–21
Dierickx K (1980) Immunocytochemical localization of the vertebrate cyclic nonapeptide neurohypophyseal hormones and neurophysins. Int Rev Cytol 62:119–185
Dockray GJ (1976) Immunochemical evidence of cholecystokinin-like peptides in brain. Nature 264:568–570
Goltermann NR, Rehfeld JF, Petersen HR (1980) Concentration and in vivo synthesis of cholecystokinin in subcortical regions of the rat brain. J Neurochem 35:479–483
Grube D (1980) Immunoreactivities of gastrin (G-) cells. II. Non-specific binding of immunoglobulins to G-cells by ionic interactions. Histochemistry 66:149–167
Grube D, Weber E (1980) Immunoreactivities of gastrin (G-) cells. I. Dilution-dependent staining of G-cells by antisera and non-immune sera. Histochemistry 65:223–237
Hökfelt T, Lunberg JM, Schultzberg M, Johansson O, Ljundahl A, Rehfeld J (1980) Coexistence of peptides and putative transmitters in neurons. In: Costa E, Trabucchi M (eds) Neural peptides and neuronal communication. Raven Press, New York, p 23
Larsson LI, Rehfeld JF (1979) Localization and molecular heterogeneity of cholecystokinin in the central and peripheral nervous system. Brain Res 165:201–218
Lorén I, Alumets J, Håkanson R, Sundler F (1979) Distribution of gastrin and CCK-like peptides in rat brain. Histochemistry 59:249–257
Martin R, Voigt KH (1981) Enkephalins co-exist with oxytocin and vasopressin in nerve terminals of rat neurohypophysis. Nature 289:502–504
Rehfeld JF (1978) Localization of gastrins to neuro- and adenohypophysis Nature 271:771–773
Robberecht P, Deschodt-Lanckman M, Vanderhaeghen JJ (1978) Demonstration of biological activity of brain gastrin-like peptide material in the human: its relationship with the COOH-terminal octapeptide of cholecystokinin. Proc Natl Acad Sci USA 75:524–528
Sternberger LA, Hardy jr PH, Cuculis JJ, Meyer (1970) The unlabeled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Toubeau G, Desclin J, Parmentier M, Pasteeis JL (1979) Compared localizations of prolactin-like and adrenocorticotropin immunoreactivities within the brain of the rat. Neuroendocrinology 29:374–384
Vandesande F, Dierickx K, De Mey J (1977) The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis. Cell Tissue Res 180:443–452
Vanderhaeghen JJ, Signeau JC, Gepts W (1975) New peptide in the vertebrate CNS reacting with antigastrin antibodies. Nature 257:604–605
Vanderhaeghen JJ, Lotstra F, De Mey J, Gilles C (1980) Immunohistochemical localization of cholecystokinin and gastrin-like peptide in the brain and hypophysis of the rat. Proc Natl Acad Sci USA 77:1190–1194
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vanderhaeghen, J.J., Lotstra, F., Vandesande, F. et al. Coexistence of cholecystokinin and oxytocin-neurophysin in some magnocellular hypothalamo-hypophyseal neurons. Cell Tissue Res. 221, 227–231 (1981). https://doi.org/10.1007/BF00216585
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216585