Summary
Calcium (Ca) and Magnesium (Mg) are determined by atomic absorption flame spectrometry in isolated cardiac mitochondria from mice receiving subcutaneous injections of DL-isoproterenol HC1 (ISO), and in mitochondria of untreated controls. In the controls, mitochondria were isolated in the presence or absence of ruthenium red. On the absence of ruthenium red in the isolation medium, mitochondrial Ca levels increase by about 300%, while levels of Mg remain unchanged. Focal myocardial necrosis following a single ISO-injection is shown by electron microscopy. Ca and Mg levels are largely unaffected by a single dose of ISO until 24 h after the injection. A slight increase in Ca occurs in the 48 h samples. When multiple injections of ISO are given every 12th hour for 48 h, 72 h and 96 h, respectively, endogenous Ca and Mg increase significantly. It is suggested that this increase might be associated with ISO-induced cardiac hypertrophy rather than with the pharmacological effects of ISO per se.
Similar content being viewed by others
References
Bloom S, Cancilla PAC (1969) Myocytolysis and mitochondrial calcification in rat myocardium after isoproterenol. Am J Pathol 54:373–391
Bloom S, Davis DL (1972) Calcium as mediator of isoproterenol-induced myocardial necrosis. Am J Pathol 69:459–470
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem 72:247–254
Carafoli E (1975) Mitochondria, Ca2+ transport and the regulation of heart contraction and metabolism. J Mol Cell Cardiol 7:83–89
Carafoli E, Crompton M (1978) The regulation of intracellular calcium by mitochondria. In: Scarpa A, Carafoli E (eds) Calcium transport and cell function. Annals of the New York Academy of Sciences, 307. New York, N Y, pp 269–283
Carafoli, E, Lehninger AL (1971) A survey of the interaction of calcium ions with mitochondria from different tissues and species. Biochem J 122:681–690
Crompton M, Carafoli E (1979) The measurements of Ca2+ movements in mitochondria. In: Fleischer S, Packer L (eds) Biomembranes, Part G. Methods in Enzymology, LVI. Acad Press, New York San Francisco London, pp 338–352
Crompton M, Capano M, Carafoli E (1976) The sodium-induced efflux of calcium from heart mitochondria. A possible mechanism for the regulation of mitochondrial calcium. Eur J Biochem 69:453–462
Csapo Z, Dusek J, Rona G (1972) Early alterations of the cardiac muscle cells in isoproterenol-induced necrosis. Arch Pathol 93:356–365
Dhalla NS (1976) Involvement of membrane systems in heart failure due to intracellular calcium overload and deficiency. J Mol Cell Cardiol 8:661–667
Dhalla NS, Ziegelhoffer A, Harrow JAC (1977) Regulatory role of membrane systems in heart function. Can J Physiol Pharmacol 55:1211–1234
Dhalla NS, Das PK, Sharma GP (1978) Subcellular basis of cardiac contractile failure. J Mol Cell Cardiol 10:363–385
Fleckenstein A, Janke J, Døring HJ, Pachinger O (1973) Ca overload as the determinant factor in the production of catecholamine-induced myocardial lesions. In: Bajusz E, Rona G (eds) Recent advances in studies on cardiac structure and metabolism. Vol 2: Cardiomyopathies, Urban & Schwarzenberg München Berlin Wien, pp 455–466
Fleckenstein A, Janke J, Døring HJ, Leder O (1974) Myocardial fiber necrosis due to intracellular Ca overload —a new principle in cardiac pathophysiology. In: Dhalla NS (ed) Recent advances in studies on cardiac structure and metabolism. Vol 4: Myocardial biology, Urban & Schwarzenberg, München Berlin Wien, pp 563–580
Justesen N-PB, Sætersdal T, Engedal H (1978) Calcium in isolated mitochondria from the left ventricular wall. Histochemistry 56:203–211
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137A
Kitazawa T (1976) Physiological significance of Ca uptake by mitochondria in the heart in comparison with that by cardiac sarcoplasmic reticulum. J Biochem 80:1129–1147
Lehninger AL, Reynafarje B, Vercesi A, Tew WP (1978) Transport and accumulation of calcium in mitochondria. In: Scarpa A, Carafoli E (eds) Calcium transport and cell function. Annals of the New York Academy of Sciences, 307: New York, N Y, pp 160–174
Lie R, Pryme I, Sætersdal T (1980) The effects of isoproterenol and dexamethasone on cardiac ribosomes and polysomes. (in press)
McDonald JM, Bruns DE, Jarett L, Davies JE (1977) A rapid microtechnique for the preparation of biological material for the simultaneous analysis of calcium, magnesium and protein. Ann Biochem 82:485–492
Moore CL (1971) Specific inhibition of mitochondrial Ca++ transport by ruthenium red. Biochem Biophys Res Comm 42:298–305
Nirdlinger EL, Bramante PO (1974) Subcellular myocardial ionic shifts and mitochondrial alterations in the course of isoproterenol-induced cardiopathy of the rat. J Mol Cell Cardiol 6:49–60
Reynolds ES (1963) The use of lead citrate as an electron-opaque stain in electron microscopy. J Cell Biol 17:208
Rona G, Chappel CI, Balazs T, Gaudry R (1959) An infarct-like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Arch Pathol 67:443–455
Rossi CS, Washington FD, Carafoli E (1973) The effect of ruthenium red on the uptake and release of Ca2+ by mitochondria. Biochem Biophys Res Comm 50:846–852
Sætersdal T, Engedal H, Røli J, Myklebust R (1980) Calcium and magnesium levels in isolated mitochondria from human cardiac biopsies. Histochemistry 68:8
Tomlinson CW, Lee SL, Dhalla NS (1976) Abnormalities in heart membranes and myofibrils during bacterial infective cardiomyopathy in the rabbit. Circ Res 39:82–92
Varley KG, Dhalla NS (1973) Excitation-contraction coupling in heart. XII. Subcellular calcium transport in isoproterenol-induced myocardial necrosis. Exp Mol Pathol 19:94–105
Author information
Authors and Affiliations
Additional information
This work was supported by grants from The Norwegian Council on Cardiovascular Disease and from The Norwegian Research Council for Science and the Humanities
Rights and permissions
About this article
Cite this article
Sætersdal, T., Engedal, H., Røli, J. et al. Calcium and magnesium levels in isolated cardiac mitochondria from mice injected with isoproterenol. Cell Tissue Res. 215, 13–19 (1981). https://doi.org/10.1007/BF00236245
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00236245