Summary
Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes ofClivia andLilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternac as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes ofCanna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex “zone of exclusion” which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.
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The work has been supported in part by the Deutsche Forschungsgemeinschaft.
We thank Miss Marianne Winter for technical assistance and Drs. H. Falk (University of Freiburg) and H. H. Mollenhauer (Purdue University, Lafayette, U.S.A.) as well as W. Herth and H. Zentgraf for helpful discussions. We are indebted to Dr. G. Schreiber (University of Freiburg) for kind provision with the Morris hepatoma material and to Mrs. Doris Stach for the drawing.
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Franke, W.W., Kartenbeck, J., Krien, S. et al. Inter- and intracisternal elements of the Golgi apparatus. Z.Zellforsch 132, 365–380 (1972). https://doi.org/10.1007/BF02450714
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DOI: https://doi.org/10.1007/BF02450714