Summary
To investigate calcium distribution in cardiac muscle cells, two methods, one using oxalate and another using lead acetate, were used concomitantly to determine the subcellular localization of calcium. Particular attention was paid to the specificity of the methods employed. Chemical and physical analyses of the electron-opaque deposits of the reaction end-products was performed by chelation with EGTA or X-ray and electron diffraction. Results obtained show that the distribution of the calcium deposits in the cardiac muscle cells is more complex than that described for striated muscle fibers. The implications of these findings are discussed and an original viewpoint on the calcium distribution and movement in cardiac muscle cells is presented.
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References
Azzi, A., Chance, B.: Localization of primary steps in ion movements in membranes. Ann. N.Y. Acad. Sci. 147, 812–815 (1969).
Barr, L.: Electrical transmission between the cells of vertebrate cardiac muscle. In: Comparative physiology of the heart: current trends. (F. V. McCann ed.), pp. 102–110. Basel: Birkhäuser 1969.
Baskin, R. J., Deamer, D. W.: Comparative ultrastructure and calcium transport in heart and skeletal muscle microsomes. J. Cell Biol. 43, 610–617 (1969).
Bianchi, C. P.: Cell calcium. London: Butterworths 1968.
Bowmann, R. W.: Mitochondrial connections in canine myocardium. Tex. Rep. Biol. Med. 25, 517–524 (1967).
Carasso, N., Favard, P.: Mise en évidence du calcium dans les myonèmes pédonculaires de ciliés péritriches. J. Microscopie 5, 759–770 (1966).
Catalina, F., Cifuentes, L.: Calcium oxalate: crystallographic analysis in solid aggregates in urinary sediments. Science 169, 183–184 (1970).
Chimoskey, J. E., Gergely, J.: Effect of ions on sarcoplasmic reticulum fragments. Arch. Biochem 128, 601–605 (1968).
Constantin, L. L., Franzini-Armstrong, C., Podolsky, R. J.: Localization of calcium-accumulating structures in striated muscle fibres. Science 147, 158–159 (1965).
Diculescu, I., Ionescu, N., Popescu, L. M.: In press.
Fanburg, B., Finkel, R. M., Martonosi, A.: The role of calcium in the mechanism of relaxation of cardiac muscle. J. biol. Chem 239, 2298–2306 (1964).
Fawcett, D. W.: The sarcoplasmic reticulum of skeletal and cardiac muscle. Circulation 24, 336–348 (1961).
—, McNutt, M. S.: The ultrastructure of the cat myocardium. I. Ventricular papillary muscle. J. Cell Biol. 42, 1–45 (1969).
Forssmann, W. G., Girardier, L.: Untersuchungen zur Ultrastruktur des Rattenherzmuskels mit besonderer Berücksichtigung des sarcoplasmatischen Retikulums. Z. Zellforsch. 72, 249–275 (1966).
Frank, G. B.: Calcium and the initiation of contraction. Circulat. Res. 14, Suppl. II (1964).
Grossman, A., Furchgott, R. F.: The effects of external calcium concentration on the distribution and exchange of calcium in resting and beating guinea pig auricles. J. Pharmacol. exp. Ther. 143, 107–114 (1964).
Hasselbach, W.: Relaxation and the sarcotubular calcium pump. Fed. Proc. 23, 909–912 (1964).
Heuman, H. G.: Calciumakkumulierende Strukturen in einem glatten Wirbellosenmuskel. Protoplasma (Wien) 67, 111–115 (1969).
Langer, G. A.: Ion fluxes in cardiac excitaion and contraction and their relation to myocardial contractility. Phys. Rev. 48, 708–757 (1968).
Legato, M. J., Langer, G. A.: The subcellular localization of calcium ion in mammalian myocardium. J. Cell Biol. 41, 401–423 (1969).
Luft, J.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Nayler, W. G.: Effect of caffeine on cardiac contractile activity and radiocalcium movement. Amer. J. Physiol. 204, 969–974 (1963).
—: Calcium and other divalent ions in contraction of cardiac muscle. In: Muscle (W. M. Paul, E. E. Daniel, C. M. Kay and G. Monckton eds.), p. 184. London: Pergamon Press, Discussion 1965.
—: Calcium exchange in cardiac muscle: a basic mechanism of drug action. Amer. Heart J. 73, 379–394 (1967).
—, Chipperfield, D.: Effect of skeletal muscle potentiators on Ca++ in cardiac sarcoplasmic reticulum. Amer. J. Physiol. 217, 609–614 (1969).
—, Emery, P. E.: Nicotine reduced contractures in depolarized cardiac muscle. Arch. int. Pharmacodyn. 153, 283–288 (1965).
Nelson, D. A., Benson, E. S.: On the structural continuities of the transverse tubular system of rabbit and human myocardial cells. J. Cell Biol. 16, 297–313 (1963).
Niedergerke, R.: The rate of action of calcium ions on the contraction of the heart. J. Physiol. (Lond.) 138, 506–515 (1957).
Page, S.: Structure of the sarcoplasmic reticulum in vertebrate muscle. Brit. med. Bull. 24, 170–173 (1968).
Pease, A. C., Jenden, D. J., Howell, J. N.: Calcium uptake in glycerol-extracted rabbit psoas muscle fibres. II. Electron microscopic localization of uptake sites. J. cell. comp. Physiol. 65, 141–145 (1965).
Rabinovitz, M., Nair, K. G., Zak, R.: Cellular and subcellular basis of cardiac hypertrophy. Med. Clin. N. Amer. 54, 211–220 (1970).
Reynafarje, B., Lehninger, A. L.: High affinity and low affinity binding of Ca++ by rat liver mitochondria. J. biol. Chem. 244, 584–593 (1969).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963).
Scarpa, A., Azzone, G. F.: Ion transport in liver mitochondria. VI. The role of surface binding on aerobic Ca++ translocation. J. biol. Chem. 243, 5132–5138 (1968).
Sillen, L., Martell, A.: Stability constants of metal-ion complexes, Special Publ. 17, London: Chemical Society 1964.
Simpson, F. O., Oertelis, S. J.: The fine structure of sheep myocardial cells; sarcolemmal invaginations and the transverse tubular system. J. Cell Biol. 12, 91–100 (1962).
Smith, D. S.: Skeletal, cardiac, and smooth muscle. In: Electron microscopic anatomy (S. Kurtz, ed.), pp. 267–294. New York: Academic Press 1964.
Sperelakis, N.: Lack of electrical coupling between contiguous myocardial cells in vertebrate hearts. In: Comparative physiology of the heart: current trends (F. V. McCann, ed.), pp. 135–165. Basel: Birkhäuser 1969.
Tobian, L., Duke, M.: Increased calcium and water concentrations in the left ventricle of hypertensive rats. Amer. J. Physiol. 217, 522–524 (1969).
Weber, A., Herz, R., Reiss, J.: The nature of the cardiac relaxing factor. Biochim. biophys. Acta (Amst.) 131, 188–194 (1967).
Winegrad, S.: Autographic studies of intracellular calcium in frog skeletal muscle. J. gen. Physiol. 48, 455–479 (1965).
—, Shanes, A. M.: Calcium flux and contractility in guinea pig atria. J. gen. Physiol. 45, 371–394 (1962).
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Diculescu, I., Popescu, L.M., Ionescu, N. et al. Ultrastructural study of calcium distribution in cardiac muscle cells. Z. Zellforsch. 121, 181–198 (1971). https://doi.org/10.1007/BF00340671
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DOI: https://doi.org/10.1007/BF00340671