Summary
The median eminence and the pars nervosa of Rana esculenta have a different structure.
The median eminence has 2 different zones: the outer zone situated near the pars distalis and the inner zone under the ependyme. In the outer zone there are, according to the size and the shape of the granules, 5 types of nerve terminals.
1. Endings containing spherical fine dense granules of 800 to 1000 Å in diameter; 2. Endings with spherical granules from 1000 to 1200 Å in diameter; 3. Endings with granules of irregular shape which are bigger than the former (1200 to 1600 Å); 4. Endings with spherical dense granules of about 1200 to 1800 Å in diameter; 5. A few endings containing only clear vesicles. Type 3 and type 4 endings are probably neurosecretory.
The inner zone contains numerous neurosecretory fibres. They are of two types: one with big granules (1600–2400 Å), the second with smaller granules (1300–2000 Å). Non-neurosecretory fibres have also been observed.
The pars nervosa contains two principal types of neurosecretory fibres: one with dense granules of 1600 to 2400 Å in diameter, the other with lighter granules of about 1300 to 2000 Å. In the external zone lining the pars intermedia, aminergic fibres with fine granules have been observed.
Résumé
L'éminence médiane et la pars nervosa de Rana esculenta diffèrent du point de vue de leur structure.
L'éminence médiane se compose de 2 zones différentes: la zone externe placée près du lobe distal et la zone interne située sous l'épendyme. Dans la zone externe, on distingue, d'après la taille et la forme des grains de sécrétion, 5 types de terminaisons.
1. des terminaisons avec de fins granules sphériques denses de 800 à 1000 Å de diamètre; 2. des terminaisons avec des granules de 1000 à 1200 Å de diamètre; 3. des terminaisons avec des grains de forme irrégulière de diamètre supérieur aux précédents (1200 à 1600 Å); 4. des terminaisons avec de volumineux grains denses sphériques d'environ 1200 à 1800 Å de diamètre; 5. un petit nombre de terminaisons ne contenant que des vésicules. Les terminaisons des catégories 3 et 4 sont probablement du type neurosécrétoire.
La zone interne contient de nombreuses fibres neurosécrétrices. Elles sont de 2 types, l'une avec de gros granules (1600–2400 Å), l'autre avec des granules moins volumineux (1300–2000 Å). Des fibres non neurosécrétrices ont également été observées.
Dans la pars nervosa, on rencontre deux types principaux de fibres neurosécrétrices, l'une avec des grains denses de 1600 à 2400 Å de diamètre, l'autre avec des grains moins denses d'environ 1300 à 2000 Å de diamètre. Dans la zone externe bordant la pars intermedia des fibres aminergiques avec de fines granulations ont été observées.
Similar content being viewed by others
Bibliographie
Barer, R., Lederis, K.: Ultrastructure of the rabbit neurohypophysis with special reference to the release of hormones. Z. Zellforsch. 75, 201–239 (1966).
—, Heller, H.: The isolation, identification and properties of the hormonal granules of the neurohypophysis. Proc. roy. Soc. B. 158, 388–416 (1963).
Barry, J., Cotte, G.: Etude préliminaire au microscope électronique de l'éminence médiane du cobaye. Z. Zellforsch. 53, 714–724 (1961).
Bern, H. A., Nishioka, R. S.: Fine structure of the median eminence of some passerine birds. Proc. Zool. Soc. (Calcutta) 18, 107–119 (1965).
Bindler, E., la Bella, F. S., Sanwall, M.: Isolated nerve endings (neurosecretosomes) from the posterior pituitary. Partial separation of vasopressine and ocytocin and the isolation of microvesicles. J. Cell Biol. 34, 185–205 (1967).
Campbell, D. J., Holmes, R. L.: Further studies on the neurohypophysis of the hedgehog (Erinaceus europaeus). Z. Zellforsch. 75, 35–46 (1966).
Craigie, E. H.: Vascular connections of the hypophysis in the leopard frog (Rana pipiens). Anat. Rec. 74, 61–69 (1939).
Dawson, A.B.: Hypothalamo-hypophysial relationships in Rana pipiens demonstrated by Gomori's chrome-alum hematoxylin method. Anat. Rec. 112, 443–444 (1952).
—: Morphological evidence of a possible functional interrelationship between the median eminence and the pars distalis of the Anuran hypophysis. Anat. Rec. 128, 77–89 (1957).
De Robertis, E.: Ultrastructure and function in some neurosecretory systems. In: Neurosecretion, ed. by H. Heller and R. B. Clark, p. 3–20. London: Academic Press 1962.
Diepen, R.: Vergleichend-anatomische Untersuchungen über das Hypophysen-Hypothalamus-System bei Amphibien und Reptilien. Verh. Anat. Ges. Erg. Bd. Anat. Anz. 50, 79–89 (1952).
Dierickx, K.: The nerve fibres controlling the gonadotropic activity of the hypophysis of Rana temporaria. Z. Zellforsch. 63, 938–949 (1964).
—: The origin of the aldehyde negative nerve fibres of the median eminence of the hypophysis: a gonadotropic centre. Z. Zellforsch. 66, 504–518 (1965).
—: The function of the hypophysis without preoptic neurosecretory control. Z. Zellforsch. 78, 114–130 (1967).
—, Abeele, A., van den: On the relations between the hypothalamus and the anterior pituitary in Rana temporaria. Z. Zellforsch. 61, 78–87 (1959).
Doerr-Schott, J., Follenius, E.: Localisation des fibres aminergiques dans l'hypophyse de Rana esculenta. Etude autoradiographique au microscope électronique. C. R. Acad. Sci. (Paris) 269, 737–740 (1969).
—: Innervation de l'hypophyse intermédiaire de Rana esculenta et identification des fibres aminergiques par autoradiographie au microscope électronique. Z. Zellforsch. 106, 99–118 (1970a).
—: Identification et localisation des fibres aminergiques dans l'éminence médiane de Rana esculenta par autoradiographie au microscope électronique. Z. Zellforsch. 111, 427–436 (1970b).
Duffy, P. E., Menefee, M.: Electron microscopic observations of neurosecretory granules, nerve and glial fibers, and blood vessels in the median eminence of the rabbit. Amer. J. Anat. 117, 251–285 (1965).
Gerschenfeld, H. M., Tramezzani, J. H., De Robertis, E.: Ultrastructure and function in neurohypophysis of the toad. Endocrinology 66, 741–762 (1960).
Green, J. D.: Vessels and nerves of Amphibian hypophysis. Anat. Rec. 99, 21–53 (1947).
Hartmann, J. F.: Electron microscopy of the neurohypophysis in normal and histamine-treated rats. Z. Zellforsch. 48, 291–308 (1958).
Hirano, T.: Neurohypophysial hormones in the median eminence of the bullfrog turtle an duck. Endocr. jap. 13, 59–74 (1966).
Holmes, R. L.: The neurohypophysis of the foetal monkey. Z. Zellforsch. 69, 288–295 (1966).
Jørgensen, C. B.: Brain pituitary relationships in Amphibians, Birds and Mammals: on the origin and nature of the neurons by which hypothalamic control of pars distalis functions are mediated. Arch. Anat. micr. Morph. exp. 54, 261–276 (1965).
—, Larsen, L. O., Rosenkilde, P., Wingstrand, K. G.: Effect of extirpation of median eminence on function of pars distalis of the hypophysis in the toad Bufo bufo (L.). Comp. Biochem. Physiol. 1, 38–43 (1960).
Kobayashi, H., Bern, H. A., Nishioka, R. S., Hyodo, Y.: The hypothalamo-hypophyseal neurosecretory system of the parakeet: Melopsittacus undulatus. Gen. comp. Endocr. 1, 545–564 (1961).
—, Matsui, T.: Synapses in the rat and pigeon median eminences. Endocr. jap. 14, 279 (1967).
La Bella, F. S., Beaulieu, G., Reiffenstein, R. J.: Evidence for the existence of separate vasopressin and ocytocin-containing granules in the neurohypophysis. Nature (Lond). 193, 173 (1962).
Lederis, K.: An electron microscopical study of the human neurohypophysis. Z. Zellforsch. 65, 847–868 (1965).
- Heller, H.: Intracellular storage of vasopressin and oxytocin. Internat. Congr. of Endocrinology p. 115–116, Copenhagen (1960).
Matsui, T.: Fine structure of the posterior median eminence of the pigeon Columba livia domestica. J. Fac. Sci. Univ. Tokyo 11, 49–70 (1966).
—: Fine structure of the median eminence of the rat. J. Fac. Sci. Uni. Tokyo 11, 71–96 (1966).
Mazzi, V.: Alcuni casi di rigenerazione del lobo nervoso dell'ipofisi nel Tritone crestato in seguito all' introduzione di una laminetta di celloidina fra eminenza mediate adenoipofisi. Boll. Soc. ital. Biol. sper. 34, 377–379 (1958).
Monroe, B. G., Scott, D. E.: Ultrastructural changes in the neural lobe of the hypophysis of the rat during lactation and suckling. J. Ultrastruct. Res. 14, 497–517 (1966).
Nishioka, R. S., Howard, A., Bern, H. A., Mewaldt, R.: Ultrastructural aspects of the neurohypophysis of the white-crowned sparrow, Zonotrichia leucophrys gambelli, with special reference to the relation of neurosecretory axons to ependyma in the pars nervosa. Gen. comp. Endocr. 4, 304–313 (1964).
Oota, Y.: Fine structure of the median eminence and the pars nervosa of the mouse. J. Fac. Sci. Uni. Tokyo 10, 155–168 (1963).
—: Fine structure of the median eminence and the pars nervosa of the turtle, Clemmys japonica. J. Fac. Sci Uni. Tokyo 10, 169–170 (1963).
—, Kobayashi, H.: Fine structure of the median eminence and the pars nervosa of the bullfrog, Rana catesbeiana. Z. Zellforsch. 60, 667–687 (1963).
Rinne, U. K.: Ultrastructure of the median eminence of the rat. Z. Zellforsch. 74, 98–122 (1966).
Rodríguez, E. M.: Differences between median eminence and neural lobe in Amphibians. Z. Zellforsch. 74, 308–316 (1966).
—: Ultrastructure of the neurohaemal region of the toad median eminence. Z. Zellforsch. 93, 182–212 (1969).
—, La Pointe, J.: Histology and ultrastructure of the neural lobe of the lizard, Klauberina riversiana. Z. Zellforsch. 95, 37–57 (1969).
—, Piezzi, R. S.: The effects of adenohypophysectomy on the hypothalamic hypophysial neurosecretory system and the adrenal gland of the toad Bufo arenarum Hensel. Z. Zellforsch. 80, 93–107 (1967).
—, Vega, J. A., La Malfa, J. A.: The different origins of the hypothalamo-hypophysial tracts of the toad Bufo arenarum H. Gen. comp. Endocr. 9, 487 (1967).
—: The different origins of the neurosecretory hypothalamo-hypophysial tracts of the toad Bufo arenarum Hensel. Gen. comp. Endocr. 14, 248–255 (1970).
Röhlich, P., Vigh, B., Teichmann, I., Aros, B.: Electron microscopy of the median eminence of the rat. Acta Biol. Acad. Sci. hung. 15, 431–457 (1965).
Roth, L. M., Luse, S. A.: Fine structure of the neurohypophysis of the opossum. J. Cell Biol. 20, 3, 459–472 (1964).
Schneider, A. P. G., McCann, S. M.: Possible role of dopamine as transmitter to promote discharge of LH releasing factor. Endocrinology 85, 121–132 (1969).
Shiozaki, N.: Studies on hypothalamic neurosecretion in Amphibians under several experimental conditions. Kitakanto med. J. 9, 197–212 (1959).
Smoller, G. G.: Ultrastructural studies on the developing neurohypophysis of the pacific treefrog Hyla regilla. Gen. comp Endocr. 7, 44–73 (1967).
Uemura, H., Kobayashi, H.: The hypothalamo-hypophysial neurosecretory system of the neotenous salamander, Necturus maculosus. Acta Anat. nippon 37, 218–229 (1962).
Voitkevich, A. A.: Neurosecretory control of the Amphibian metamorphosis. Gen. comp. Endocr., Suppl. 1, 133–147 (1962).
Wilson, L. D., Weinberg, J. A., Bern, H. A.: The hypothalamic neurosecretory system of the tree frog Hyla regilla. J. comp. Neurol. 107, 253–271 (1957).
Zambrano, D., De Robertis, E.: Ultrastructural changes of the neurohypophysis of the rat after castration. Z. Zellforsch. 86, 14–25 (1968).
Ziegler, B.: Licht- und Elektronenmikroskopische Untersuchungen an pars intermedia und Neurohypophyse der Ratte. Z. Zellforsch. 59, 486–506 (1963).
Author information
Authors and Affiliations
Additional information
Je tiens à exprimer mes vifs remerciements, à Monsieur le Professeur E. Follenius pour l'intérêt constant qu'il prend à ce travail. Je remercie également Madame R. O. Clauss, collaboratrice technique et Madame Schwoerer, photographe, pour leur aide précieuse.
Rights and permissions
About this article
Cite this article
Doerr-Schott, J. Etude au microscope électronique de la neurohypophyse de Rana esculenta L.. Z. Zellforsch. 111, 413–426 (1970). https://doi.org/10.1007/BF00342491
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00342491