Abstract
A tandem DNA duplication carried on a ColE1-derived plasmid segregates at high frequency upon generalized transduction by phage P22 HT. Transductional segregation of the plasmid-borne duplication can be promoted either by RecA or by the Erf function of P22, indicating that transductional segregation is a consequence of the recombination events that re-circularize the plasmid in the recipient cell. RecA-mediated and Erf-mediated transduction give similar frequencies of duplication segregation and yield the same types of segregation products, indicating that two distinct recombination machineries (RecA + RecBCD and Erf + RecBCD) perform similar or identical recombination reactions on plasmid DNA substrates transduced by bacteriophage P22 HT.
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Received: 4 September 1997 / Accepted: 23 March 1998
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Garzón, A., Cano, D. & Casadesús, J. The P22 Erf protein and host RecA provide alternative functions for transductional segregation of plasmid-borne duplications. Mol Gen Genet 259, 39–45 (1998). https://doi.org/10.1007/s004380050786
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DOI: https://doi.org/10.1007/s004380050786