Abstract
Kin28/Ccl1, a cyclin-dependent kinase, is essential for the in vivo phosphorylation of the C-terminal domain of the largest subunit of RNA polymerase II in Saccharomyces cerevisiae. In a search for mutations co-lethal with a thermosensitive kin28 mutation, we have identified genes whose products interact functionally with Kin28. In the present work, we have studied a new complementation group of synthetic lethal mutations. The corresponding gene, RIG2, encodes a predicted RING finger protein. Rig2 is likely to be a homolog of MAT1 of higher eukaryotes which forms a ternary complex with MO15(cdk7) and cyclin H. Our genetic data suggest that Rig2 is a component of transcription factor TFIIH. Transcription activity in a rig2-ts mutant is impeded at restrictive temperature. However, none of the rig2-ts mutants obtained was UV sensitive, suggesting that Rig2 is dispensable for nucleotide excision repair.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 2 October 1996 / Accepted: 27 January 1997
Rights and permissions
About this article
Cite this article
Faye, G., Simon, M., Valay, J. et al. Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast. Mol Gen Genet 255, 460–466 (1997). https://doi.org/10.1007/s004380050518
Issue Date:
DOI: https://doi.org/10.1007/s004380050518