Summary
Virulent mutants of phage Mu have been isolated after mutagenesis. The virulent phenotype results from most probably 2 mutations located in the c-A region of the Mu genome.
Vir mutants are trans-dominant; they induce the resident prophage upon infection in broth of any Mu lysogen. They however form plaques only on certain lysogens, that are monolysogenic for a mutant prophage. We further isolated secondary mutations in Mu Vir which suppress the virulent phenotype.
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References
Adams, M.H.: In: Bacteriophages. New York: Interscience Publishers 1959
Appleyard, R.K.: Segregation of lambda lysogenicity during bacterial recombination in Escherichia coli K12. Genetics 39, 429–439 (1954)
Botstein, D., Lew, K.K., Jarvik, V., Swanson, C.A. Jr.: Role of antirepressor in the bipartite control of repression and immunity by bacteriophage P22. J. molec. Biol. 91, 439–462 (1975)
Bukhari, A.I.: Bacteriophage Mu as a transposition element. Ann. Rev. Genet. 10, 389–412 (1976)
Campbell A.: Sensitive mutants of bacteriophage lambda. Virology 14, 22–32 (1961)
Chesney, R.H., Scott, J.R.: Superinfection immunity and prophage repression in phage P1-II Mapping of the immunity-difference and ampicillin-resistant loci of P1 and Øamp. Virology 67, 375–384 (1975)
Couturier, M.: The intergration and excision of the bacteriophage Mu-1. Cell 7, 155–163 (1976)
Couturier, M., van Vliet, F.: Vegetative recombination in bacteriophage Mu-1. Virology 60, 1–8 (1974)
Faelen, M., Toussaint, A.: Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage. Virology 54, 117–124 (1973)
Faelen, M., Toussaint, A., Couturier, M.: Mu-1 promoted integration of a λgal phage in the chromosome of E. coli K12. Molec. gen. Genet. 113, 367–370 (1971)
Faelen, M., Toussaint, A., de Lafonteyne, J.: Model for the enhancement of λ-gal integration into partially induced Mu-1 lysogens. J. Bact. 121, 873–882 (1975)
Fry, B.A.: Conditions for the infection of E. coli with lambda phage and for establishment of lysogeny. J. gen. Microbiol. 21, 676–689 (1959)
Gottesman, M., Yarmolinsky, M.: Integration negative mutants of bacteriophage lambda. J. molec. Biol. 31, 487–505 (1968)
Howe, M.M.: Prophage deletion mapping of bacteriophage Mu-1. Virology 54, 93–101 (1973)
Howe, M.M., Bade, E.G.: Molecular biology of bacteriophage Mu. Science 190, 624–632 (1975)
Lennox, E.S.: Transduction of linked genetic characters of host by bacteriophage P1. Virology 1, 190–206 (1955)
Levine, M., Truesdell, S., Ramakrishnan, T., Bronson, M.J.: Dual control of lysogeny by bacteriophage P22: an antirepressor locus and its controlling elements. J. molec. Biol. 91, 421–438 (1975)
Magazin, M., Howe, M.M., Allet, B.: Partial correlation of the genetic and physical maps of bacteriophage Mu. Virology 77, 677–688 (1977)
Miller, J.H.: Experiments in molecular biology. New York: Cold Spring Harbor Press 1972
Scott, J.R.: Superinfection immunity and prophage repressor in phage P1. Virology 65, 173–178 (1975)
Scott, J.R., Laping, J.L., Chesney, R.H.: A P1 virulent mutant at a new map location. Virology 78, 346–348 (1977)
Taylor, A.L.: Bacteriophage induced mutation in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 50, 1043–1051 (1963)
Toussaint, A., Faelen, M.: Connecting two unrelated DNA sequences with a Mu dimer. Nature (Lond.) New Biol. 242 1–4 (1973)
Toussaint, A., Faelen, M., Bukhari, A.I.: Mu mediated illegitimate recombination as a part of the Mu lytic cycle. Insertion sequences, plasmids and episomes (Bukhari, A.I., Shpairo, J., Adhya, S., eds.) New York: Cold Spring Harbor Press 1977
Waggoner, B.T., Gonzales, N.S., Taylor, A.L.: Isolation of heterogeneous circular DNA from induced lysogenes of bacteriophage Mu-1. Proc. nat. Acad. Sci. (Wash.) 71, 1255–1259 (1974)
Wandersman, C., Yarmolinsky, M.: Bipartite control of immunity conferred by the related heteroimmune plasmid prophages P1 and P7 (formerly øamp). Virology 77, 386–400 (1977)
Weigle, J., Meselson, M., Paigen, K.: Density alteration associated with transduction ability in the bacteriophage lambda. J. molec. Biol. 1, 379–386 (1959)
West, B.W., Scott, J.R.: Superinfection immunity and prophage repression in phage P1 and P7. III. Induction by virulent mutants. Virology 78, 267–276 (1977)
Westmaas, G.C., van de Maas, W.L., van de Putte, P.: Defective prophages of bacteriophage Mu. Molec. gen. Genet. 145, 81–87 (1976)
Wijffelman, C., Gassler, M., Stevens, W.F., van de Putte, P.: On the control of transcription of bacteriophage Mu. MOlec. gen. Genet. 131, 85–96 (1974)
Wijffelman, C., Lotterman, B.: Kinetics of Mu DNA synthesis. Molec. gen. Genet. 151, 169–174 (1977)
Wijffelman, C.A., Westmaas, G.C., van de Putte, P.: Vegetative recombination of bacteriophage Mu-1 in Escherichia coli. Molec. gen. Genet. 116, 40–46 (1972)
Wijffelman, C.A., Westmaas, G.C., van de Putte, P.: Similarity between the vegetative and prophage map of bacteriophage Mu-1. Virology 54, 125–134 (1973)
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van Vliet, F., Couturier, M., Desmet, L. et al. Virulent mutants of temperate phage Mu-1. Molec. Gen. Genet. 160, 195–202 (1978). https://doi.org/10.1007/BF00267481
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DOI: https://doi.org/10.1007/BF00267481