Abstract
The distribution of T-cell subsets, B cells, and class II MHC antigens was examined within the CNS of rats chronically infected withTrypanosoma brucei gambiense, using appropriate mouse monoclonal antibodies. The mononuclear infiltrates of the leptomeninges and the perivascular areas (Virchow-Robin spaces) were composed of IgM-producing plasma cells and Mott cells and T-helper/inducer cells. Cells defined phenotypically as suppressor/cytotoxic T cells were rare. Anti-Ia reactive cells were also abundant in these inflammatory lesions and in the white matter, representing Ia-expressing neuroglial cells, B cells, activated T cells, and macrophages. The Ia-positive neuroglial cells, possibly acting as accessory cells, associated with numerous T-helper/inducer cells and cells from the B-cell lineage, suggest that a T-dependent B-cell immune response can be initiated within the CNS of rats with a chronicT. b. gambiense infection.
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Alanen A, Pira U, Lassila O, Roth J, Franklin RM (1985) Mott cells are plasma cells defective in immunoglobulin secretion. Eur J Immunol 15:235–242
Barclay AN (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42:593–600
Bevan DJ, Chisholm PM (1986) Co-expression of CD4 and CD8 molecules and de novo expression of MHC class II antigens on activated rat T cells. Immunology 59:621–625
Brideau RJ, Carter PB, McMaster WR, Mason DW, Williams AF (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol 10:609–615
Brown WJ, Voge M (1982) Neuropathology of parasitic infections. Oxford University Press, Oxford New York Toronto
Cantrell DA, Robins RA, Brooks CG, Baldwin RW (1982) Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets. Immunology 45:97–103
Chirimwami B (1984) Trypanosomiase expérimentale àTrypanosoma brucei gambiense. Pathologie et pathogénie des lésions du système nerveux central chez le rat. Thesis. University of Brussels
Dallman MJ, Thomas ML, Green JR (1984) MRC OX-19, a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur J Immunol 14:260–267
Duarte AJS, Carpenter CB, Strom TB (1982) Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes. J Immunol 128:580–584
Galvão-Castro B, Hochmann A, Lambert PH (1978) The role of the host immune response in the development of tissue lesions associated with African trypanosomiasis in mice. Clin Exp Immunol 33:12–24
Greenwood BM, Whittle HC (1980) The pathogenesis of sleeping sickness. Trans R Soc Trop Med Hyg 74:716–725
Greenwood BM, Whittle HC, Oduloju KO, Dourmashkin RR (1976) Lymphocytic infiltration of the brain in sleeping sickness. Br Med J 2:1291–1292
Hunt SV, Fowler MH (1981) A repopulation assay for B and T lymphocyte stem cells employing radiation chimeras. Cell Tissue Kinet 14:511–518
Klareskog L, Holmdahl R, Larsson E, Wigzell H (1983) Role of T lymphocytes in collagen II induced arthritis in rats. Clin Exp Immunol 51:117–125
Mason DW, Gallico GG (1978) Tissue distribution and quantitation of Ia-like antigens in the rat. Eur J Immunol 8:741–748
Mason DW, Arthur RP, Dallman MJ, Green JR, Spickett GP, Thomas ML (1983) Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies. Immunol Rev 74:57–82
McMaster WR, Williams AF (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleep. Eur J Immunol 9:426–433
Molyneux DH, de Raadt P, Seed JR (1984) African human trypanosomiasis. In: Gilles HM (ed) Recent advances in tropical medicine. Churchill Livingstone Edinburgh, pp 39–62
Mulumba P, Wéry M (1983) Experimental infection with two stocks ofTrypanosoma brucei gambiense. Study of the evolution by elution rechniques of tissues. In: Gigase PLJ, VanMarck EAE (eds) From parasitic infection of parasitic disease, Contributions to Microbiology and Immunology 7. Karger, Basel, pp 120–129
Poltera AA (1980) Immunopathological and chemotherapeutic studies in experimental trypanosomiasis with special reference to the heart and brain. Trans R Soc Trop Med Hyg 74:706–715
Poltera AA (1985) Pathology of human African trypanosomiasis with reference to experimental Africa trypanosomiasis and infections of the central nervous system. Br Med Bull 41:169–174
Poltera AA, Owor R, Cox JN (1977) Pathological aspects of human African trypanosomiasis (HAT) in Uganda. A postmortem survey of fourteen cases. Virchows Arch A 373:249–265
Poltera AA, Hochmann A, Rudin W, Lambert PH (1980)Trypanosoma brucei brucei: a model for cerebral trypanosomiasis in mice — an immunological, histological and electron microscopic study. Clin Exp Immunol 40:496–507
Poltera AA, Hochmann A, Lambert PH (1982)Trypanosoma brucei gambiense: cerebral immunopathology in mice. Acta Trop 39:205–218
Simecka JW, Davis JK, Cassel GH (1986) Distribution of Ia antigens and T lymphocyte subpopulations in rat lungs. Immunology 57:93–98
Van Bogaert L, Janssen P (1957) Contribution à l'étude de la neuropathologie de la trypanosomiase humaine. Ann Soc Belg Med Trop 37:379–426
Van Marck EAE, Gigase PLJ, Beckers A, Wery M (1981) Experimental infections of laboratory rodents with recently isolated stocks ofTrypanosoma brucei gambiense: 2. Histopathological investigations. Z Parasitenkd 64:187–193
Van Marck EAE, Mulumba P, Beckers A, Gigase PLJ, Wéry M (1983) Studies of choroid plexus involvement in chronic infections of mice withTrypanosoma brucei gambiense. In: Gigase PLJ, Van Marck EAE (eds) From parasitic infection to parasitic disease, Contributions to Microbiology and Immunology 7. Karger, Basel, pp 173–182
White RAH, Mason DW, Williams AF, Galfre G, Milstein C (1978) T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med 148:664–673
Woda BA, McFadden ML, Welsh RM, Bain KM (1984) Separation and isolation of rat natural killer (NK) cells from T cells with monoclonal antibodies. J Immunol 132:2183–2184
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Anthoons, J.A.M.S., Van Marck, E.A.E., Gigase, P.L.J. et al. Immunohistochemical characterization of the mononuclear cells in the brain of the rat with an experimental chronicTrypanosoma brucei gambiense infection. Parasitol Res 75, 251–256 (1989). https://doi.org/10.1007/BF00931808
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DOI: https://doi.org/10.1007/BF00931808