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Autoantibodies to intermediate filaments in experimental infections withTrypanosoma brucei gambiense

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Abstract

Sera from rats with chronicTrypanosoma brucei gambiense infection were tested for autoantibodies by an indirect immunofluorescence assay. All the sera contained IgM autoantibodies which reacted with blood vessel walls. On cultured vascular smooth muscle cells positive sera reacted with cytoplasmic filaments which were rearranged into perinuclear coils of filaments in colcemid-pretreated smooth muscle cells. These observations strongly suggest that the cytoplasmic autoantigens are intermediate filaments (I.F.). It is probable that the anti-intermediate filament autoantibodies result from polyclonal lymphocyte activation, since in rats experimentally infected withT.b. gambiense the appearance of these autoantibodies occurs already 1 week post-infection.

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Authors and Affiliations

  1. Laboratory of Histopathology, Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerpen, Belgium

    Jacqueline A. M. S. Anthoons, E. A. E. Van Marck & P. L. J. Gigase

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  1. Jacqueline A. M. S. Anthoons
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  2. E. A. E. Van Marck
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  3. P. L. J. Gigase
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Anthoons, J.A.M.S., Van Marck, E.A.E. & Gigase, P.L.J. Autoantibodies to intermediate filaments in experimental infections withTrypanosoma brucei gambiense . Z. Parasitenkd. 72, 443–452 (1986). https://doi.org/10.1007/BF00927888

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