Summary
The fusion of the eye-antennal discs during culturein vitro has been investigated, and the complex morphogenetic movements which occur during the formation of the head capsule of the insect are described. The initial contact between the eye anlagen is by means of cell processes spanning the gap between the two discs. Subsequently the two epithelia become firmly apposed, and then the integrity of the epithelium in the region of fusion breaks down, cells appearing to move to new positions in order to form an epithelium which unites the two discs. The epithelium eventually secretes a pattern of cuticular structures which is continuous between the derivatives of the two discs. Bristles on either side of the line of fusion are perfectly aligned, and structures such as the median ocellus, which are formed jointly by the cells of the two discs, differentiate normally. This is also found when left and right eye-antennal discs of different genotypes are placed side-by-side, indicating that processes of pattern regulation can occur in culture.
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References
Bainbridge SP, Bownes M (1981) Staging the metamorphosis ofDrosophila melanogaster. J Embryol Exp Morphol 66:57–80
Birmingham L (1942) Boundaries of differentiation of cephalic imaginal discs inDrosophila. J Exp Zool 91:345–363
Bryant PJ (1978) Pattern formation in imaginal discs. In: Ashburner M, Wright TRF (eds) The genetics and biology ofDrosophila vol 2c. Academic Press, London, pp 229–335
Bullmore D (1977) The differential action of α- and β-ecdysone on the division of imaginal disc cells ofDrosophila melanogaster in vitro. Ph D Thesis University of Fribourg
Courgeon AM (1969) L'activité mitotique, en culture organotypique, dans les disques oculo-antennaires de larves deCalliphora erythrocephala Meig. (Insecte Diptère). CR Acad Sci Paris Série D 268:950–952
Dale L, Bownes M (1980) Is regeneration inDrosophila the result of epimorphic regulation? Wilhelm Roux's Arch 189:91–96
Edwards JS, Milner MJ, Chen SW (1978) Integment and sensory nerve differentiation ofDrosophila leg and wing imaginal discsin vitro. Wilhelm Roux's Arch 185:59–77
Fain MJ, Schneiderman HA (1979) Wound healing and regenerative response of fragments of theDrosophila wing imaginal disc culturedin vitro. J Insect Physiol 25:913–924
Millicovsky G, Johnston MC (1981) Active role of embryonic facial epithelium: new evidence of cellular events in morphogenesis. J Embryol Exp Morphol 63:53–66
Milner MJ (1980) Epithelial and pattern integration inDrosophila eye-antennal imaginal discs culturedin vitro. In: Kurstak E, Maramorosch K, and Dübendorfer A (eds) Invertebrate systemsin vitro. Elsevier/North Holland Biomedical Press, pp 135–148
Milner MJ, Haynie JL (1979) Fusion ofDrosophila eye-antennal imaginal discs during differentiationin vitro. Wilhelm Roux's Arch 185:363–370
Milner MJ, Sang JH (1974) Relative activities of α-ecdysone and β = ecdysone for the differentiationin vitro ofDrosophila melanogaster imaginal discs. Cell 3:141–143
Milner MJ, Bleasby AJ (1984) The alignment of imaginal anlagen during the metamorphosis ofDrosophila melanogaster. In: Balls M, Bownes M (eds) Metamorphosis. Oxford University Press [in press]
Milner MJ, Bleasby AJ, Pyott A (1983) The role of the peripodial membrane in the morphogenesis of the eye-antennal disc ofDrosophilia melanogaster. Wilhelm Roux's Arch 192:164–170
Poodry CA (1980) Imaginal discs: morphology and development. In: Ashburner M, Wright TRF (eds) The genetics and biology ofDrosophila Vol 2d. Academic Press, London, pp 407–441
Ransom R (1982) Eye and head development. In: Ransom (ed) A handbook ofDrosophila development. Elsevier Biomedical Press, Amsterdam Oxford New York, pp 123–152
Reinhardt CA, Bryant PJ (1981) Wound healing in the imaginal discs ofDrosophila II. Transmission electron microscopy of normal and healing wing discs. J Exp Zool 216:45–61
Reinhardt CA, Hodgkin NM, Bryant PJ (1977) Wound healing in the imaginal discs ofDrosophila 1. Scanning electron microscopy of normal and healing wing discs. Dev Biol 60:238–257
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Schneider I (1964) Differentiation of larvalDrosophila eye-antennal discsin vitro. J Exp Zool 156:91–104
Sprey TE, Oldenhave M (1974) A detailed organ map of the wing disk ofCalliphora erythrocephala. Netherlands J Zool 24:291–310
Shields G, Sang JH (1977) Improved medium for culture ofDrosophila embryonic cells. Drosophila Inform Serv 52:161
Steinberg AG (1943) The development of the wild type and Bar eyes ofDrosophila melanogaster. Canad J Res Sec D 21:227–283
Tucker JB (1967) Changes in nuclear structure during binary fission in the ciliateNassula. J Cell Sci 2:481–498
Waterman RE (1976) Topographical changes along the neural fold associated with neurulation in the hamster and mouse. Am J Anat 146:151–171
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Milner, M.J., Bleasby, A.J. & Pyott, A. Cell interactions during the fusionin vitro ofDrosophila eye-antennal imaginal discs. Wilhelm Roux' Archiv 193, 406–413 (1984). https://doi.org/10.1007/BF00848232
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DOI: https://doi.org/10.1007/BF00848232