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Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay

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Abstract

Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subsequent quantitation of the cytokinins by single-ion monitoring on a gas chromatography-mass spectrometry system. The results were compared with those obtained by radioimmunoassay as well as a bioassay. Quantitations by gas chromatography-mass spectrometry and radioimmunoassay correlated well, whereas the estimates by the bioassay gave considerably lower values.

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Abbreviations

i6Ado:

isopentenyladenosine

FW:

fresh weight

GC-MS:

gas chromatography-mass spectrometry

RIA:

radioimmunoassay

SIM:

single-ion monitoring

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Authors and Affiliations

  1. Max-Planck-Institut für Biochemie, D-8033, Martinsried, Federal Republic of Germany

    D. Ernst, W. Schäfer & D. Oesterhelt

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  1. D. Ernst
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  2. W. Schäfer
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  3. D. Oesterhelt
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Ernst, D., Schäfer, W. & Oesterhelt, D. Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay. Planta 159, 216–221 (1983). https://doi.org/10.1007/BF00397527

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  • DOI: https://doi.org/10.1007/BF00397527

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