Abstract
The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
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Abbreviations
- R:
-
red light
- FR:
-
far-red light
- GA3 :
-
gibberellic acid
- GA:
-
gibberellin
- Pfr :
-
far-red absorbing form of phytochrome
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Blakeley, S.D., Thomas, B., Hall, J.L. et al. Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid. Planta 158, 416–421 (1983). https://doi.org/10.1007/BF00397734
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DOI: https://doi.org/10.1007/BF00397734