Abstract
Particulate preparations from Pisum sativum. were able to incorporate [14C]glucose from UDP-[14C]glucose into oligosaccharide-linked lipids was formed by an oligosaccharide chain containing 7-8 glucose residues linked to dolichol, presumably via a pyrophosphate. The polymer was identified as a membrane-bound glucoprotein that could be solubilized by Triton X-100. SDS gel electrophoresis showed that a polypeptide with an apparent molecular weight of 13,000 could be glucosylated from dolichyl-phosphate-glucose. This was coincident with the electrophoretic mobility of the β subunit of the pea lectin in the same system. The glucosylated protein was solubilized from the membranes by sonication and showed the same carbohydrate-binding ability as pea lectins. These results strongly suggest that pea lectins can be glucosylated by the lipid intermediate pathway.
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Abbreviations
- BSA:
-
Bovine serum albumin
- Dol:
-
dolichol
- SDS:
-
sodium dodecyl sulfate
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Pont Lezica, R., Romero, P.A. & Hopp, H.E. Glucosylation of membrane-bound proteins by lipid-linked glucose. Planta 140, 177–183 (1978). https://doi.org/10.1007/BF00384918
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DOI: https://doi.org/10.1007/BF00384918