Summary
A method is described by which a numerical value can be assigned to the amount of DNA-synthesis shown graphically by population-histograms obtained by Feulgen cytophotometry. The index appeared to give a reasonable measure of DNA-synthetic activity in populations having very low, moderate and high mitotic indices and closely followed labelling indices obtained by autoradiography. Thus the advantage of a numerical value is that the DNA-synthetic activities in different populations can be compared for statistical analysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brues AM, Marble BB (1937) Analysis of mitosis in liver restoration. J Exp Med 65:15–28
Chayen J, Bitensky L, Butcher RG (1973) Practical histochemistry. John Wiley, New York London
Consden R, Doble A, Glynn LE, Nind AP (1971) Production of a chronic arthritis with ovalbumin. Ann Rheum Dis 30:307–315
Coulton LA (1977) Temporal relationship between glucose 6-phosphate dehydrogenase activity and DNA-synthesis. Histochemistry 50:207–215
Deeley EM (1955) An integrating microdensitometer for biological cells. J Sci Instrum 32:263–267
Deeley EM, Davies HG, Chayen J (1957) The DNA content of cells in the root of Vicia faba. Exp Cell Res 12:582–591
Dumonde DC, Glynn LE (1962) The production of arthritis in rabbits by an immunological reaction to fibrin. Br J Exp Pathol 43:373–383
Fukuda M, Böhm N, Fujita S (1978) Cytophotometry and its biological applications. Prog Histochem Cytochem 11:1–119
Henderson B, Glynn LE, Bitensky L, Chayen J (1981) Evidence for cell division in synoviocytes in acutely inflamed rabbit joints. Ann Rheum Dis 39:241–247
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coulton, L.A., Henderson, B. & Chayen, J. The assessment of DNA-synthetic activity. Histochemistry 72, 91–99 (1981). https://doi.org/10.1007/BF00496783
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496783