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Influence of photoperiod and medium formulation on peanut somatic embryogenesis

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Abstract

Somatic embryos were produced from peanut (Arachis hypogaea L.) immature zygotic cotyledons. Comparisons were made of the level of α-naphthaleneacetic acid during induction, nitrogen formulation of the medium, and photoperiod. Over 70% embryogenesis was obtained regardless of NAA level used. Percent embryogenesis and number of embryos were markedly lower in explants induced on NAA compared to 2,4-D. Embryo production was not greatly affected by either the use of Murashige & Skoog versus Finer & Nagasawa salts or light versus dark culture conditions. However, embryo morphology was noticeably affected by photoperiod. Embryos produced under a 16 h photoperiod were tough, woody and difficult to separate for subsequent germination and conversion. Those produced under a 0-h photoperiod were succulent and pliable.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

MS:

Murashige & Skoog (1962)

B5:

Gamborg et al. (1968)

picloram:

4-amino-3,5,6-trichloropicolinic acid

FN:

Finer & Nagasawa (1988)

2,4,5-T:

2,4,5-trichlorophenoxyacetic acid

References

  • Ammirato P (1974) Bot Gaz 135:328–337

    Google Scholar 

  • Arcioni S, Damiani F, Pezzotti M, Lupotto E (1990) In: Bajaj Y (ed) Biotechnology in Agriculture and Forestry 10 Legumes and Oil Seed Crops I, Springer Verlag, New York, pp 197–241.

    Google Scholar 

  • Arumugan N, Bhojwani S (1990) Can J Bot 68:487–491

    Google Scholar 

  • Baker C, Wetzstein H (1992) Plant Cell Rep 11:71–75

    Google Scholar 

  • Barwale UB, Kerns HR, Widholm JM (1986) Planta 167:473–481

    Google Scholar 

  • Durham E, Parrott W (1992) Plant Cell Rep 11:122–125

    Google Scholar 

  • Feng X, Jiang X, Shao Q (1989) Sci in China 32:58–63

    Google Scholar 

  • Finer J, Nagasawa A (1988) Plant Cell Tiss Org Cult 15:125–136

    Google Scholar 

  • Gamborg O, Miller R, Ojima K (1968) Exp Cell Res 50:150–158

    Google Scholar 

  • Ghazi TD, Cheema HV, Nabors MW (1986) Plant Cell Rep 5:452–456

    Google Scholar 

  • Gleddie S, Keller W, Setterfield G (1983) Can J Bot 61:656–666

    Google Scholar 

  • Hazra S, Sathaye S, Mascarenhas A (1989) Bio/Technology 7:949–951

    Google Scholar 

  • Kysely W, Jacobsen H (1990) Plant Cell Tiss Org Cult 20:7–14

    Google Scholar 

  • Lazzeri P, Hildebrand D, Collins G (1987a) Plant Cell Tiss Org Cult 10:197–208

    Google Scholar 

  • Lazzeri P, Hildebrand D, Collins G (1987b) Plant Cell Tiss Org Cult 10:209–220

    Google Scholar 

  • McKently A (1991) In Vitro Cell Dev Biol 27P:197–200

    Google Scholar 

  • Michler C, Bauer E (1991) Plant Sci 77:111–118

    Google Scholar 

  • Michler C, Lineberger D (1987) Plant Cell Tiss Org Cult 11:189–207

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–479

    CAS  Google Scholar 

  • Ozias-Akins P (1989) Plant Cell Rep 8:217–218

    Google Scholar 

  • Ranch JP, Oglesby L, Zielinski AC (1985) In Vitro Cell Dev Bio 21:653–658

    Google Scholar 

  • Sellars R, Southward G, Phillips G (1990) Crop Sci 30:408–414

    Google Scholar 

  • Stuart D, Strickland S (1984a) Plant Sci Lett 34:165–174

    Google Scholar 

  • Stuart D, Strickland S (1984b) Plant Sci Lett 34:175–181

    Google Scholar 

  • Trigiano RN, Beaty RM, Graham ET (1988) Plant Cell Rep 7:148–150

    Google Scholar 

  • Walker K, Sato S (1981) Plant Cell Tiss Org Cult 1:109–121

    Google Scholar 

  • Wetzstein HY, Baker CM (1993) Plant Sci (in press).

Download references

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Authors and Affiliations

  1. Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602-7273, Athens, GA, USA

    Charleen M. Baker, J. Austin Burns & Hazel Y. Wetzstein

Authors
  1. Charleen M. Baker
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  2. J. Austin Burns
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  3. Hazel Y. Wetzstein
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Communicated by W. Parrott

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Baker, C.M., Burns, J.A. & Wetzstein, H.Y. Influence of photoperiod and medium formulation on peanut somatic embryogenesis. Plant Cell Reports 13, 159–163 (1994). https://doi.org/10.1007/BF00239884

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  • DOI: https://doi.org/10.1007/BF00239884

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