Abstract
The mitochondrial genome of the selfed progeny of a plant regenerated from long-term somatic tissue culture displays specific structural rearrangements characterized by the appearance of novel restriction fragments. A mitochondrial DNA library was constructed from this selfed progeny in the SalI site of cosmid pHC79 and the novel fragments were subsequently studied. They were shown to arise from reciprocal recombination events involving DNA sequences present in the parental plant. The regions of recombination were sequenced and the nucleotide sequences were aligned with those of the presumptive parental fragments. We characterized an imperfect short repeated DNA sequence, 242 bp long, within which a 7-bb DNA repeat could act as a region of recombination. The use of PCR technology allowed us to show that these fragments were present in both parental plants and tissue cultures as low-abundance sequence arrangements.
Similar content being viewed by others
References
André C, Levy A, Walbot V (1992) Trends Genet 8:128–132
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Bonen L, Bird S (1988) Gene 73:47–56
Bonen L, Boer PH, Gray MW (1984) EMBO J 3:2531–2536
Bonen L, Bird S, Belanger L (1990) Plant Mol Biol 15:793–795
Brown PTH (1991) IAPTC Newslett 66:14–25
Day A, Ellis THN (1985) Curr Genet 9:671–679
Dennis ES, Brettell RIS, Peacock WJ (1987) Mol Gen Genet 210:181–183
Devereux J, Haeberli P, Smithies O (1984) Nucleic Acids Res 12:387–395
Escote-Carlson LJ, Gabay-Laughnan S, Laughnan JR (1990) Mol Gen Genet 223:457–464
Fauron CMR, Havlik M, Brettell RIS (1990) Genetics 124:423–428
Grunstein M, Hogness D (1975) Proc Natl Acad Sci USA 72:3961–3965
Harada T, Sato T, Asaka D, Matsukawa I (1991) Theor Appl Genet 81:157–161
Hartmann C, De Buyser J, Henry Y, Falconet D, Lejeune B, Benslimane AA, Quétier F, Rode A (1987) Plant Sci 53:191–198
Hartmann C, Henry Y, De Buyser J, Aubry C, Rode A (1989) Theor Appl Genet 77:169–175
Hartmann C, De Buyser J, Henry Y, Morère-Le Paven MC, Dyer T, Rode A (1992) Curr Genet 21:515–520
Isaac PG, Brennicke A, Dunbar SM, Leaver CJ (1985) Curr Genet 10:321–328
Joyce PBM, Spencer DF, Gray MW (1988) Plant Mol Biol 11:833–843
Kadowaki K, Suzuki T, Kazama S (1990) Mol Gen Genet 224:10–16
Karp A (1992) Oxford Surveys Pl Mol Cell Biol 7:1–58
Kettner G, Kelly TJ (1976) Proc Natl Acad Sci USA 73:1102–1106
Khasanov FK, Zvingila DJ, Zainullin AA, Prozorov AA (1992) Mol Gen Genet 234:494–497
Larkin PJ, Scowcroft WR (1981) Theor Appl Genet 60:197–214
Lee M, Phillips RL (1988) Annu Rev Plant Physiol Plant Mol Biol 39:413–437
Lonsdale DM, Hodge TP, Fauron CMR (1984) Nucleic Acids Res 12:9249–9261
Lonsdale DM, Brears T, Hodge TP, Melville SE, Rottman WH (1988) Phil Trans R Soc Lond B 319:149–163
Mackenzie SA, Pring DR, Bassett MJ, Chase CD (1988) Proc Natl Acad Sci USA 85:2714–2717
Manna E, Brennicke A (1986) Mol Gen Genet 203:377–381
Mita S, Rizzuto R, Moraes CT, Shanske S, Arnaudo E, Fabrizi GM, Koga Y, DiMauro S, Schon EA (1990) Nucleic Acids Res 18:561–567
Morère-Le Paven MC, Henry Y, De Buyser J, Corre F, Hartmann C, Rode A (1992a) Theor Appl Genet 85:1–8
Morère-Le Paven MC, De Buyser J, Henry Y, Corre F, Hartmann C, Rode A (1992b) Theor Appl Genet 85:9–14
Murashige T, Skoog F (1962) Physiol Plant 13:473–497
Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–512
Newton KJ, Knudsen C, Gabay-Laughnan S, Laughnan JR (1990) Plant Cell 2:107–113
Ozias-Akins P, Tabaeizadeh Z, Pring DR, Vasil IK (1988) Curr Genet 13:241–245
Palmer JD, Shields CR (1984) Nature 307:437–440
Peschke VM, Phillips RL, Gengenbach GG (1987) Science 238:804–807
Quétier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) In: Groot G, Hall T (eds) Molecular form and function of plant genomes, vol 83. Plenum, New York, pp 413–420
Rode A, Hartmann C, Dron M, Picard E, Quétier F (1985) Theor Appl Genet 71:320–324
Rode A, Hartmann C, Falconet D, Lejeune B, Quétier F, Benslimane AA, Henry Y, De Buyser J (1987) Curr Genet 12:369–376
Sanger F, Nicklen F, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Shirzadegan M, Palmer JD, Christey M, Earle ED (1991) Plant Mol Biol 16:21–37
Small ID, Isaac PG, Leaver CJ (1987) EMBO J 6:865–869
Small ID, Suffolk R, Leaver CJ (1989) Cell 58:69–76
Southern E (1975) J Mol Biol 98:503–517
Author information
Authors and Affiliations
Additional information
Communicated by C.J. Leaver
Rights and permissions
About this article
Cite this article
Hartmann, C., Récipon, H., Jubier, MF. et al. Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat. Curr Genet 25, 456–464 (1994). https://doi.org/10.1007/BF00351786
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00351786