Summary
The enzymatic hydrolysis of well-defined dextran fractions is studied. The results show that the rate constants of degradation are proportional to the molecular weight for molecular weights higher than 5000; for lower molecular weights the rate constants decrease gradually. Enzymatic hydrolysis of dextran is not a statistical reaction, since the individual rate constants of bond cleavage increase from the midpoint of the chain towards both ends. The results are discussed in comparison with acid hydrolysis of dextran, and the characteristic differences between both reactions are pointed out.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
BASEDOW, A.: Proceedings of the 2. Deutsche Diskussionstagung für Anwendung der HPLC, Waters Associates, October 1977, Königstein/Taunus, pp. 175–200
BASEDOW, A., EBERT, K.H., EDERER, H., HUNGER, H.: Makromol. Chem. 177, 1501 (1976)
BASEDOW, A., EBERT, K.H., EDERER, H.: Macromolecules 11, 774 (1978)
BOURNE, E., HUTSON, D., WEIGEL, H.: Biochem. J. 85, 158 (1962)
EBERT, K.H., EDERER, H., BASEDOW, A.: Angew. Chem. 91, 341 (1979)
GARG, S., STIVALA, S.: J. Polym. Sci. Poly. Phys. Ed. 16, 1419 (1978)
HUTSON, D., WEIGEL, H.: Biochem. J. 88, 588 (1963)
JANSON, J., PORATH, J. in ‘Methods in Enzymology VIII’ 615 (1966), edited by COLOWICK and KAPLAN, Academic Press, New York
JEANES, A., WILHAM, C., JONES, R., TSUCHIYA, H., RIST, C.: J. Am. Chem. Soc. 75, 5911 (1953)
LARM, O., LINDBERG, B., SVENSSON, S.: Carbohyd. Res. 20, 39 (1971)
WALKER, G.: Int. Rev. Biochem. 16, 75 (1978)
WALKER, G., PULKOWNIK, A.: Carbohyd. Res. 36, 53 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Basedow, A.M. Studies on the enzymatic hydrolysis of dextran. Polymer Bulletin 2, 337–342 (1980). https://doi.org/10.1007/BF00266711
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00266711