Summary
Deficiency of inorganic phosphate caused the hyper production of invertase and the derepression of acid phosphatase in a continuous culture ofSaccharomyces carlsbergensis. The specific invertase activity was 40,000 enzyme units per g dry cell weight at a dilution rate lower than 0.05 h−1 with a synthetic glucose medium of which the molecular ratio of KH2PO4 to glucose was less than 0.006. This activity is eight fold higher than in a batch growth and 1.5 fold as much as the highest enzyme activity observed so far in a glucose-limited continuous culture.
For the hyper production of invertase, it is necessary to culture the yeast continuously by keeping the Nyholm's conservative inorganic phosphate concentration at less than 0.2 m mole per g dry weight cell. The derepression of acid phosphatase brought about by phosphate deficiency, was similar in both batch and continuous cultures.
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Abbreviations
- D:
-
dilution rate of continuous culture (h−1)
- Ei :
-
invertase concentration in culture (enzyme unit l−1)
- Ep :
-
acid phosphatase concentration in culture (enzyme unit l−1)
- P:
-
inorganic phosphate concentration in culture (mM)
- S:
-
glucose concentration in culture (mM)
- X:
-
cell concentration in culture (g dry weight cell l−1)
- μ:
-
specific rate of growth (h−1)
- f:
-
feed
- 0:
-
initial value
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Toda, K., Yabe, I. & Yamagata, T. Invertase and phosphatase of yeast in a phosphate-limited continuous culture. European J. Appl. Microbiol. Biotechnol. 16, 17–22 (1982). https://doi.org/10.1007/BF01008237
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DOI: https://doi.org/10.1007/BF01008237