Effect of hydrolysing and oxidizing agents on the composition and degradation of wheat straw monosaccharides

Summary

Wheat straw (WS) was treated with 5% sodium hydroxide, ozone and 5% sulfur dioxide at 70°C for 72 h, and the effect of treatments on monosaccharide composition and in vitro degradability by rumen microorganisms was studied. The major sugars, glucose and xylose, comprising about 90% of the total monosaccharides in the untreated WS were mainly confined to the cell walls. SO₂ exerted the greatest solubilizing effect, followed by ozone and NaOH; the respective values for the solubilized cell wall polysaccharides were: 26, 12 and 4.4%. One third of the total phenolics was oxidized by ozone, whereas, SO₂ exerted mostly a solubilizing effect on this fraction, converting 75% of it into soluble phenolics. In the NaOH treated WS 41% of the total phenolics were soluble, as compared to 22% in the untreated. The in vitro digestibility of monosaccharides in the untreated WS were initially high: 50% and 58% for xylose and glucose, respectively and 63% to 80% for the minor sugars. The SO₂ treatment resulted in an overall increase in digestibility of monosaccharides with values lying in the range of 90%. Sodium hydroxide was more efficient than ozone in enhancing the degradability of xylan and total sugars. The digestibility of cell wall sugars was increased from 52.4% to 84.4%, 63.4% and 72.3% by SO₂, O₃ and NaOH treatments respectively. Based on the present findings, it appears that wheat straw cell wall components are more sensitive to hydrolytic than to oxidative processes aimed at increasing its degradability by rumen microorganisms. SO₂ exerted on WS a multi-effect which was particularly suitable for increasing the digestibility of monosaccharides.