Abstract
Genetic variability among Hoplostethus atlanticus collected from two spawning grounds east and west of New Zealand was examined using DNA fingerprints as revealed by hybridization with three clonal probes: 33.15, M13 and 3′HVR. The 33.15 and 3′HVR fingerprints revealed a complex pattern of restriction fragments, apparently refecting a multi-locus system of highly variable minisatellite alleles similar to the pattern of alleles reported in other vertebrates. The M13 fingerprints revealed a distinct pattern of restriction fragments of high molecular weight, reflecting a single-locus system that overlapped with the family of minisatellite alleles observed in 33.15 fingerprints. In a sample of 12 orange roughy collected on a single regional spawning site, the average percent similarity of 33.15 fingerprints was 21.15% (SD=17.75), the average percent similarity of 3′HVR fingerprints was 14.32% (SD=14.45) and the inferred average allelic frequency of the M13 single-locus system was 0.071. A comparison of 33.15 and M13 fingerprints from two distant spawning sites ground New Zealand revealed no obvious regional differences. The variability of orange roughy fingerprints was so great, however, that regional comparisons could not be considered conclusive indicators of genetic identity. Our results provide a preliminary assessment of the power and pitfalls of using DNA-level markers for the population analysis of marine fish.
Similar content being viewed by others
Literature cited
Allen, R. W., Bliss, B., Pearson, A. (1989). Characteristics of a DNA probe (pa3′HVR) when used for paternity testing. Transfusion, Philad. 29: 477–485
Burke, T., Bruford, M. W. (1987). DNA fingerprinting in birds. Nature, Lond. 327: 149–152
Castelli, M., Philippart, J.-C. (1992). DNA fingerprinting in fish: a new generation of genetic markers (in preparation)
Church, G. M., Gilbert, W. (1984). Genomic sequencing. Proc. natn. Acad. Sci. U.S.A. 81: 1991–1995
Fowler, S. J., Gill, P., Werrett, D. J., Higgs, D. R. (1988). Individual specific DNA fingerprints from a hypervariable region proble: alpha-globin 3′HVR. Hum. Genetics (Suppl.) 79: 142–146
George, M., Lequarre, A.-S., Castelli, M., Hanset, R., Vassart, G. (1988). DNA fingerprinting in domestic animals using four different minisatellite probes. Cytogenet. Cell Genet. 47: 127–131
Gilbert, D. A., Lehman, N., O'Brien, S. J., Wayne, R. K. (1990). Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature, Lond. 344: 764–767
Hallerman, E. M., Beckman, J. S. (1988). DNA-level polymorphism as a tool in fisheries science. Can. J. aquat. Sciences 45: 1075–1087
Harris, A. S., Bieger, S., Doyle, R. W., Wright, J. M. (1991). DNA fingerprinting of tilapia, Oreochromis niloticus, and its application to aquaculture genetics. Aquaculture, Amsterdam 92: 157–163
Hill, W. G. (1987). DNA fingerprints applied to animal and bird populations. Nature, Lond. 327: 98–99
Jarman, A. P., Wells, R. A. (1989). Hypervariable minisatellites: recobinators or innocent bystanders? Trends Genetics 5: 367–371
Jeffreys, A. J., Wilson, V., Thein, S. L. (1985a). Hypervariable ‘minisatellite’ regions in human DNA. Nature, Lond. 316: 67–73
Jeffreys, A. J., Wilson, V., Thein, S. L. (1985b). Individual-specific ‘fingerprints’ of human DNA. Nature, Lond. 316: 76–79
Jeffreys, A. J., Wong, Z., Wilson, V., Patel, I., Neuman, R., Royle, N., Armour, J. A. L. (1989). Applications of multilocus and single-locus minisatellite DNA probes in forensic medicine. Cold Spring Harbor Press, Cold Spring Harbor, New York (Banbury Report 32: DNA technology and forensic science)
Lander, E. (1989). Population genetic considerations in the forensic use of DNA typing. In: Ballantyne, J., Sensabaugh, G., Witkoski, J. (eds.) DNA technology and forensic science. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, p 143–156
Lynch, M. (1990). The similarity index and DNA fingerprinting. Molec, Biol. Evolut. 7: 478–484
Nakamura, Y., Leppert, M., O'Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlin, E., White, R. (1987). Variable number of tandem repeat (VNTR) markers for human gene mapping. Science, N.Y. 235: 1616–1622
Ovenden, J. R., Smolenski, A. J., White, W. G. (1989). Mitochondrial DNA restriction site variation in Tasmanian populations of orange roughy (Hoplostethus atlanticus) a deep-water marine teleost. Aust. J. mar. Freshwat. Res. 40: 1–9
Packer, C., Gilbert, D. A., Pusey, A. E., O'Brien, S. J. (1991). A molecular genetic analysis of kinship and cooperation in African lions. Nature, Lond. 351: 542–545
Robertson, D. A. (1991). The New Zealand orange roughy fishery — an overiew. In: Abel, K., Williams, M., Smith, P. (eds.) Proceedings of the Southern Trawl Fisheries Conference, Melbourne, May 1990. Bureau of Rural Resources, Canberra, p. 38–48
Robertson, D. A., Grimes, P. J. (1983). The New Zealand orange roughy fishery. In: Taylor, J. L., Baird, G. (eds.) New Zealand finfish fisheries: the resources and their management. New Zealand Trade Publications, Wellington, p. 15–20
Smith, A. D. M. (1991). As many red fishes as live in the sea: orange roughy in Australia. In: Abel, K., Williams, M., Smith, P. (eds.) Proceedings of the Southern Trawl Fisheries Conference, Melbourne, May 1990. Bureau of Rural Resources, Canberra, p. 49–54
Smith, P. J. (1986). Genetic similarity between samples of the orange roughy (Hoplostethus atlanticus) from the Tasman Sea, Southwest Pacific Ocean and the North-east Atlantic Ocean. Mar. Biol. 91: 173–180
Smith, P. J., Francis, R. I. C. C., McVeagh, M. (1991). Loss of genetic diversity due to fishing pressure. Fish. Res. 10: 309–316
Taggert, J. B., Ferguson, A. (1990). Hypervariable minisatellite DNA single locus probes for the Atlantic salmon, Salmo salar L. J. Fish Biol. 37: 991–993
Turner, B. J., Elder, J. F., Laughlin, T. F. (1989). DNA fingerprinting of fishes: a general method using oligonucleotide probes. DNA Fingerprinting News 4: 15–16
Turner, B. J., Elder, J. F. Jr., Laughlin, T. F., Davis, W. P. (1990). Genetic variation in clonal vertebrates detected by simple-sequence DNA fingerprinting. Proc. natn. Acad. Sci. USA 87: 5653–5657
Vassart, G., Georges, M., Monsieur, R., Brocas, H., Lequarre, A. S., Christophe, D. (1987). A sequence of M13 phage detects hypervariable minisatellites in human and animal DNA. Science, N.Y. 235: 683–684
Waldman, J. R., Grossfield, J., Wirgin, I. (1988). Review of stock discrimination techniques for striped bass. N. Am. J. Fish. Mgmt 8: 410–425
Wetton, J. H., Carter, R. E., Parkin, D. T., Walters, D. (1987). Demographic study of a wild house sparrow population by DNA fingerprinting. Nature, Lond. 327: 147–149
Wright, S. (1978). Evolution and the genetic of populations. Vol. IV. Variability within and among natural populations. University of Chicago Press, Chicago, Ilinois
Author information
Authors and Affiliations
Additional information
Communicated by G. F. Humphrey, Sydney
Rights and permissions
About this article
Cite this article
Baker, C.S., MacCarthy, M., Smith, P.J. et al. DNA fingerprints of orange roughy, Hoplostethus atlanticus: a population comparison. Marine Biology 113, 561–567 (1992). https://doi.org/10.1007/BF00349699
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00349699