Summary
Raman microprobe spectroscopy was used to study the concentration of lignocellulosics in the cell corner middle lamella. Spectra obtained from 1.6 μm regions, from 30 cell corner middle lamellae of both birch and spruce, showed the presence of lignin. However, the relative concentration of lignin to cellulose varied considerably. These results corroborate the view expressed in previous reports of the need for caution in using the lignin concentration values of cell corner middle lamella as a internal reference for studying the variation of lignin concentration in other morphological regions of the cell wall, such as secondary cell wall layers.
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Tirumalai, V.C., Agarwal, U.P. & Obst, J.R. Heterogeneity of lignin concentration in cell corner middle lamella of white birch and black spruce. Wood Sci.Technol. 30, 99–104 (1996). https://doi.org/10.1007/BF00224961
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DOI: https://doi.org/10.1007/BF00224961