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Inhibitory effects of undecayed wood and the detection of Postia placenta using the enzyme-linked immunosorbent assay

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Summary

Pulverized, undecayed wood was shown to be, in general, inhibitory to attempts to use enzyme-linked immunosorbent assay (ELISA) in detecting and quantifying the decay fungus Postia placenta (Fr.) M. Lars. et Lomb. Nevertheless, the assay was still able to adequately detect the presence of P. placenta at a greater than 1: 10,000 dilution even in the presence of 0.1 g/ml spruce wood. The use of polyvinyl pyrrolidone did not improve ELISA performance but adsorbent polystyrene had some effect on the assay sensitivity.

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References

  • Clark, M. F.; Adams, A. N. 1977: Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34: 475–483

    CAS  PubMed  Google Scholar 

  • Goodell, B.; Jellison, J. 1986: Detection of a brown-rot fungus using serological assays. Internat. Res. Group on Wood Pres. Working group 1a. Document IRG/WP/1305

  • Loomis, W. D.; Lile, J. D.; Sandstrom, R. P.; Burbott, A. J. 1979: Adsorbent polystyrene as an aid in plant enzyme isolation. Phytochemistry 18:1049–1054

    Article  CAS  Google Scholar 

  • Voller, A.; Bidwell, D. E.; Bartlett, A. 1977: The enzyme-linked immunosorbent assay (ELISA). A review with a bibliography of microplate application. Guernsey: Flowline Publ.

    Google Scholar 

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Jellison, J., Goodell, B. Inhibitory effects of undecayed wood and the detection of Postia placenta using the enzyme-linked immunosorbent assay. Wood Sci.Technol. 23, 13–20 (1989). https://doi.org/10.1007/BF00350602

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  • DOI: https://doi.org/10.1007/BF00350602

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