Abstract
It is generally accepted that during fast growth of Escherichia coli, the time (D) between the end of a round of DNA replication and cell division is constant. This concept is not consistent with the fact that average cell mass of a culture is an exponential function of the growth rate, if it is also accepted that average cell mass per origin of DNA replication (Mi) changes with growth rate and negative exponential cell age distribution is taken into account. Data obtained from cell composition analysis of E. coli OV-2 have shown that not only (Mi) but also D varied with growth rate at generation times (τ) between 54 and 30 min. E. coli OV-2 is a thymine auxotroph in which the replication time (C) can be lengthened, without inducing changes in τ, by growth with limiting amounts of thymine. This property has been used to study the relationship between cell size and division from cell composition measurements during growth with different amounts of thymine. When C increased, average cell mass at the end of a round of DNA replication also increased while D decreased, but only the time lapse (d) between the end of a replication round and cell constriction initiation appeared to be affected because the constriction period remained fairly constant. We propose that the rate at which cells proceed to constriction initiation from the end of replication is regulated by cell mass at this event, big cells having shorter d times than small cells.
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Abbreviations
- OD450 and OD630 :
-
Optical density at a given wavelength in nm
References
Adams MH (1959) Bacteriophages. Interscience, New York
Breitman TR, Maury PB, Toal JN (1972) Loss of deoxyribonucleic acid-thimine during thymine starvation of Escherichia coli. J Bacteriol 112:646–648
Bremer H (1986) A stochastic process determines the time at which cell division begins in Escherichia coli. J Theor Biol 118:351–365
Bremer H, Churchward G (1977) Deoxyribonucleic acid synthesis after inhibition of initiation of rounds of replication in Escherichia coli B/r. J Bacteriol 130:692–697
Bremer H, Churchward G, Young R (1979) Relation between growth and replication in bacteria. J Theor Biol 81:533–545
Brooks RF (1981) Variability in the cell cycle and the control of cell proliferation. In: John PCL (ed) The cell cycle. Cambridge University Press, Cambridge London New York New Rochelle Melbourne Sidney, pp 276–281
Churchward G, Estiva E, Bremer H (1981) Growth rate-dependent control of chromosome replication initiation in Escherichia coli. J Bacteriol 145:1232–1238
Cooper S (1988) What is the bacterial growth law during the division cycle? J Bacteriol 170:5001–5005
Donachie WD, Begg KJ, Vicente M (1976) Cell length, cell growth and cell division. Nature (Lond) 264:328–333
Fantes P, Nurse P (1977) Control of cell size at division in fission yeast by a growth-modulated size control over nuclear division. Exp Cell Res 107:377–386
Grossman N, Ron EZ (1989) Apparent minimal size required for cell division in Escherichia coli. J Bacteriol 171:80–82
Helmstetter CE, Pierucci O, Weinberger M, Holmes M, Tang M (1979) Control of cell division in Escherichia coli. In: Sokatch IR, Ornston LN (eds) The bacteria, vol 7 Academic Press, New York, pp 517–579
Lark KG, Repko T, Hoffman EJ (1963) The effect of aminoacid deprivation on subsequent deoxyribonucleic acid replication. Biochim Biophys Acta 76:9–24
Meacock PA, Pritchard RH (1975) Relationship between chromosome replication and cell division in a thymineless mutant of Escherichia coli B/r. J Bacteriol 122:931–942
Nanninga N, Woldringh CL, Koppes LJH (1982) Growth and cell division of Escherichia coli. In: Nicolini C (ed) Cell growth. Plenum Publishing Corporation, New York, pp 225–270
Navarrete MH, Cuadrado A, Cánovas JL (1983) Partial elimination of G1 and G2 periods in higher plant cells by increasing the S period. Exp Cell Res 148:273–280
Navarrete MH, Cuadrado A, Escalera M, Cánovas JL (1987) Regulation of G2 by cell size contributes to maintaining cell size variability within certain limits in higher plants J Cell Sci 87:635–641
Nurse P (1975) Genetic control of cell size at cell division in yeast. Nature (London) 652:547–551
Pato ML (1975) Alterations of the rate of movement of deoxyribonucleic acid replication forks. J Bacteriol 123:272–277
Powell EO (1956) Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol 15:492–511
Pritchard RH, Barth PT, Collins J (1969) Control of DNA synthesis in bacteria. Microbial Growth, Symp Soc Gen Microbiol 19:263–297
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Dedicated to Dr. John Ingraham to honor him for his many contributions to Science
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Puyet, A., Cánovas, J.L. Changes of Escherichia coli cell cycle parameters during fast growth and throughout growth with limiting amounts of thymine. Arch. Microbiol. 152, 578–583 (1989). https://doi.org/10.1007/BF00425490
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DOI: https://doi.org/10.1007/BF00425490