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Antigenic differences between Anabaena azollae fresh from the Azolla fern leaf cavity and free-living cyanobacteria

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Abstract

Fluorescent antibody staining indicated differences in surface antigenicity in Anabaena azollae cells fresh from the leaf cavities of the fern, Azolla caroliniana, and algae which were isolated and subcultured from this fern. Such results suggest that either changes in antigenicity occur in this phycobiont during culturing or that isolation selects for an antigenically different mutant strain capable of in vitro growth.

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Abbreviations

FA:

fluorescent antibody staining

PBS:

phosphate buffered saline

μW:

microwatt

Anti-F:

antiserum prepared against fresh cells

Anti-N:

antiserum prepared against Newton's culture

FTTC:

fluorescein isothiocyanate

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Authors and Affiliations

  1. Department of Biology, 816 Park, Virginia Commonwealth University, 23284, Richmond, VA, USA

    J. E. Gates, R. W. Fisher, T. W. Goggin & N. I. Azrolan

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  1. J. E. Gates
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  2. R. W. Fisher
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  3. T. W. Goggin
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  4. N. I. Azrolan
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Gates, J.E., Fisher, R.W., Goggin, T.W. et al. Antigenic differences between Anabaena azollae fresh from the Azolla fern leaf cavity and free-living cyanobacteria. Arch. Microbiol. 128, 126–129 (1980). https://doi.org/10.1007/BF00422316

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  • DOI: https://doi.org/10.1007/BF00422316

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